2021
Reconstructing aspects of human embryogenesis with pluripotent stem cells
Sozen B, Jorgensen V, Weatherbee B, Chen S, Zhu M, Zernicka-Goetz M. Reconstructing aspects of human embryogenesis with pluripotent stem cells. Nature Communications 2021, 12: 5550. PMID: 34548496, PMCID: PMC8455697, DOI: 10.1038/s41467-021-25853-4.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingBiomarkersBlastocystCell Culture TechniquesCell LineageEmbryo, MammalianEmbryonic DevelopmentGATA3 Transcription FactorGene ExpressionHumansModels, BiologicalPhospholipase C betaPluripotent Stem CellsSequence Analysis, RNASingle-Cell AnalysisSOXB1 Transcription FactorsSOXF Transcription FactorsConceptsStem cell modelPluripotent stem cellsStem cellsHuman embryogenesisHuman early embryogenesisSingle-cell RNA sequencingEarly embryo developmentHuman early embryo developmentPre-implantation developmentSubsets of cellsStem cell platformsCell identityEarly embryogenesisRNA sequencingCell modelTrophectoderm lineageNatural blastocystsSignaling pathwayClinical importanceCystic structuresExtended cultureEmbryogenesisPre-implantationSignificant divergenceEmbryo developmentUnfolded protein response triggers differential apoptotic mechanisms in ovaries and early embryos exposed to maternal type 1 diabetes
Okan A, Demir N, Sozen B. Unfolded protein response triggers differential apoptotic mechanisms in ovaries and early embryos exposed to maternal type 1 diabetes. Scientific Reports 2021, 11: 12759. PMID: 34140543, PMCID: PMC8211688, DOI: 10.1038/s41598-021-92093-3.Peer-Reviewed Original ResearchConceptsUnfolded protein responseProtein responseER stressDiabetes mellitusFemale mammalian reproductive systemEndoplasmic reticulum (ER)-stressMaternal type 1 diabetesEmbryonic developmentActivation of ER stressMammalian ovarian functionType 1 DMIn vivo mouse modelInduced developmental programAdult ovarian tissueEffect of DMMammalian reproductive systemEarly embryonic developmentEarly embryo developmentPre-implantation embryosType 1 diabetesApoptotic cascadeDiabetic ovariesMaternal DMActivated CHOPCaspase 12
2016
Differential expression pattern of Twist1 in mouse preimplantation embryos suggests its multiple roles during early development
Sozen B, Pehlivanoglu S, Demir N. Differential expression pattern of Twist1 in mouse preimplantation embryos suggests its multiple roles during early development. Journal Of Assisted Reproduction And Genetics 2016, 33: 1533-1540. PMID: 27544279, PMCID: PMC5125151, DOI: 10.1007/s10815-016-0794-1.Peer-Reviewed Original ResearchConceptsMouse preimplantation embryosInner cell massDouble immunofluorescence stainingCulture of embryosEarly embryo developmentExpression of Twist1Blastocyst developmentPreimplantation developmentPreimplantation embryosPresence of high glucoseEarly stages of embryosCells of embryosBlastocystImmunofluorescence stainingTwist-related protein 1Stages of embryosRegulation of TwistPurposeThe purposeUniform stainingLineage segregationTwist1 expressionProtein 1Protein levelsEmbryo developmentTwist1
2015
The p38 MAPK signalling pathway is required for glucose metabolism, lineage specification and embryo survival during mouse preimplantation development
Sozen B, Ozturk S, Yaba A, Demir N. The p38 MAPK signalling pathway is required for glucose metabolism, lineage specification and embryo survival during mouse preimplantation development. Cells And Development 2015, 138: 375-398. PMID: 26025760, DOI: 10.1016/j.mod.2015.05.002.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBlastocystBlastocyst Inner Cell MassCell DeathCell LineageEmbryonic DevelopmentFemaleGene Expression Regulation, DevelopmentalGlucoseGlucose Transporter Type 1Glucose Transporter Type 4ImidazolesMaleMAP Kinase Signaling SystemMiceMice, Inbred BALB CModels, BiologicalPregnancyProtein Kinase InhibitorsPyridinesRNA, MessengerConceptsPreimplantation developmentP38 mitogen-activated protein kinaseMitogen-activated protein kinaseLate preimplantation stagePreimplantation embryo developmentMouse preimplantation developmentExpression levelsEmbryo developmentEffect of p38 MAPK inhibitionExpression levels of NANOGEarly embryo developmentP38 MAPK inhibitionPresence of SB203580P38 MAPK signaling pathwayLevel of transcriptionIncreased cell deathSignal transduction pathwaysBlastocyst stageExpression levels of transcriptsLevels of NanogP38 MAPK pathwayPreimplantation stagesLevels of GLUT1Expression levels of Glut1MAPK signaling pathway
2014
Superovulation alters embryonic poly(A)-binding protein (Epab) and poly(A)-binding protein, cytoplasmic 1 (Pabpc1) gene expression in mouse oocytes and early embryos
Ozturk S, Yaba-Ucar A, Sozen B, Mutlu D, Demir N. Superovulation alters embryonic poly(A)-binding protein (Epab) and poly(A)-binding protein, cytoplasmic 1 (Pabpc1) gene expression in mouse oocytes and early embryos. Reproduction Fertility And Development 2014, 28: 375-383. PMID: 25034140, DOI: 10.1071/rd14106.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlastocystCells, CulturedChorionic GonadotropinDose-Response Relationship, DrugEmbryo Culture TechniquesFemaleFertility Agents, FemaleGene Expression Regulation, DevelopmentalGonadotropins, EquineMice, Inbred BALB COocytesOvulation InductionPoly(A)-Binding Protein IPoly(A)-Binding ProteinsSuperovulationTime FactorsConceptsEmbryonic poly(A)-binding proteinPregnant mare serum gonadotropinHuman chorionic gonadotrophinEarly embryo developmentAssisted reproductive technologyMII oocytesFemale miceAdministered to female miceGerminal vesicleOocyte maturationEmbryo developmentIncreased postimplantation lossDose of gonadotropinsCycling female miceDecreased implantation rateQuality of oocytesImpaired oocyte maturationGene expressionExperimental animal studiesEffects of superovulationReverse transcription-polymerase chain reactionPoly(A)-binding proteinPotential risk factorsTranscription-polymerase chain reactionImplantation rate
2013
Telomere length and telomerase activity during oocyte maturation and early embryo development in mammalian species
Ozturk S, Sozen B, Demir N. Telomere length and telomerase activity during oocyte maturation and early embryo development in mammalian species. Molecular Human Reproduction 2013, 20: 15-30. PMID: 23928157, DOI: 10.1093/molehr/gat055.Peer-Reviewed Original ResearchConceptsGranulosa cellsTelomerase activityProliferative granulosa cellsEarly embryosEarly embryo developmentTelomere lengthProliferative somatic cellsMammalian speciesEffects of oxidative stressOocyte maturationCancer cellsNon-dividing cellsStem cellsAdverse effects of oxidative stressShortened telomeresSomatic cellsOocytesEmbryo developmentAdverse effectsGranulosaGenotoxic agentsOxidative stressGermline cellsComprehensive review articleLength of telomeres