2019
High-throughput screening identifies compounds that protect RPE cells from physiological stressors present in AMD
Cai H, Gong J, Abriola L, Hoyer D, Team N, Noggle S, Paull D, Del Priore LV, Fields MA. High-throughput screening identifies compounds that protect RPE cells from physiological stressors present in AMD. Experimental Eye Research 2019, 185: 107641. PMID: 30980814, DOI: 10.1016/j.exer.2019.04.009.Peer-Reviewed Original ResearchMeSH KeywordsAntifungal AgentsApoptosisBasement MembraneCatalaseCell LineCiclopiroxCytoprotectionEpoxide HydrolasesGene Expression Regulation, EnzymologicGlutathione TransferaseHigh-Throughput Screening AssaysHumansInduced Pluripotent Stem CellsMacular DegenerationNitrosative StressOxidative StressPeroxiredoxin IIIReal-Time Polymerase Chain ReactionRetinal Pigment EpitheliumTert-ButylhydroperoxideUltraviolet RaysConceptsHigh-throughput screenHuman RPE cellsAtrophic age-related macular degenerationPrimary human RPE cellsMitochondrial functionStress-induced cell deathARPE-19Oxidative stress-induced cell deathExtracellular matrixTert-butyl hydroperoxide exposureRPE cellsOxidative damageExpression of genesInduced-pluripotent stem cellsOxidative stressSeahorse XF96 analyzerApoptosis-related genesCell viabilityGene expressionXF96 analyzerCell deathRetinal pigment epithelial cellsQuantitative reverse transcription polymerase chain reactionCiclopirox olaminePigment epithelial cells
2017
Extracellular matrix nitration alters growth factor release and activates bioactive complement in human retinal pigment epithelial cells
Fields MA, Bowrey HE, Gong J, Moreira EF, Cai H, Del Priore LV. Extracellular matrix nitration alters growth factor release and activates bioactive complement in human retinal pigment epithelial cells. PLOS ONE 2017, 12: e0177763. PMID: 28505174, PMCID: PMC5432172, DOI: 10.1371/journal.pone.0177763.Peer-Reviewed Original ResearchMeSH KeywordsAgedBiomarkersCell DifferentiationComplement ActivationComplement System ProteinsEpithelial CellsExtracellular MatrixEye ProteinsFemaleFibroblastsHumansInduced Pluripotent Stem CellsMacular DegenerationNerve Growth FactorsRetinal Pigment EpitheliumSerpinsVascular Endothelial Growth Factor A