Xinran Liu, MD, PhD
Research & Publications
Biography
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Research Summary
My research has been focused on 1) identifying synaptic proteins and its relation to synaptic structural specialization; 2) analyzing and characterizing the synaptic ultrastructural organization in genetically engineered animals; 3) correlating EM finding with electrophysiological recording on the mechanism of synaptic exocytosis and vesicle recycling. Currently we are: 1) developing electron tomography on synapses, this technique makes it possible to examine structural details in the 3D contest. 2) utilizing low-temperature methods to prepare cell and tissue samples aiming to reveal the dynamic events that associate with synaptic transmission or endocytosis.
Extensive Research Description
As one of the core facilities in the Medical School, We offer a wide range of services from conventional and immuno- electron microscopy to electron tomography. Our experienced staff routinely perform sample processing, sectioning and imaging for a set fee. Our facility is open to users who have had prior training in electron microscopy. The training on use of electron microscope and sample preparation is provided on a one-on-one basis throughout the year.
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Selected Publications
- Inguinal patch in mpox (monkeypox) virus infection and eccrine syringometaplasia: report of two cases with in situ hybridization and electron microscopy findingsRoy S, Sarhan J, Liu X, Murphy M, Bunick C, Choate K, Damsky W, McNiff J. Inguinal patch in mpox (monkeypox) virus infection and eccrine syringometaplasia: report of two cases with in situ hybridization and electron microscopy findings. British Journal Of Dermatology 2022, 188: 574-576. PMID: 36763786, DOI: 10.1093/bjd/ljac146.
- Proteasomal degradation within endocytic organelles can mediate antigen cross- presentationSengupta D, Graham M, Liu X, Cresswell P. Proteasomal degradation within endocytic organelles can mediate antigen cross- presentation. Molecular Immunology 2022, 150: 23. DOI: 10.1016/j.molimm.2022.05.080.
- Parasite‐Derived Vesicular‐Mediated Protein Export by the Human Pathogen Babesia microtiMamoun C, Thekkiniath J, Kilian N, Lawres L, Gewirtz M, Abraham A, Graham M, Liu X, Ledizet M. Parasite‐Derived Vesicular‐Mediated Protein Export by the Human Pathogen Babesia microti. The FASEB Journal 2019, 33: 649.2-649.2. DOI: 10.1096/fasebj.2019.33.1_supplement.649.2.
- Membrane adhesion dictates Golgi stacking and cisternal morphologyLee I, Tiwari N, Dunlop MH, Graham M, Liu X, Rothman JE. Membrane adhesion dictates Golgi stacking and cisternal morphology. Proceedings Of The National Academy Of Sciences Of The United States Of America 2014, 111: 1849-1854. PMID: 24449908, PMCID: PMC3918774, DOI: 10.1073/pnas.1323895111.
- A dynamin 1-, dynamin 3- and clathrin-independent pathway of synaptic vesicle recycling mediated by bulk endocytosisWu Y, O'Toole ET, Girard M, Ritter B, Messa M, Liu X, McPherson PS, Ferguson SM, De Camilli P. A dynamin 1-, dynamin 3- and clathrin-independent pathway of synaptic vesicle recycling mediated by bulk endocytosis. ELife 2014, 3: e01621. PMID: 24963135, PMCID: PMC4107917, DOI: 10.7554/elife.01621.
- Competing E3 Ubiquitin Ligases Govern Circadian Periodicity by Degradation of CRY in Nucleus and CytoplasmYoo S, Mohawk J, Siepka S, Shan Y, Huh S, Hong H, Kornblum I, Kumar V, Koike N, Xu M, Nussbaum J, Liu X, Chen Z, Chen Z, Green C, Takahashi J. Competing E3 Ubiquitin Ligases Govern Circadian Periodicity by Degradation of CRY in Nucleus and Cytoplasm. Cell 2013, 152: 1091-1105. PMID: 23452855, PMCID: PMC3694781, DOI: 10.1016/j.cell.2013.01.055.
- Nuclear localization of Klotho in brain: an anti-aging proteinGerman D, Khobahy I, Pastor J, Kuro-o M, Liu X. Nuclear localization of Klotho in brain: an anti-aging protein. Neurobiology Of Aging 2012, 33: 1483.e25-1483.e30. PMID: 22245317, PMCID: PMC3328593, DOI: 10.1016/j.neurobiolaging.2011.12.018.
- Increased anxiety‐like behavior in mice lacking the inhibitory synapse cell adhesion molecule neuroligin 2Blundell J, Tabuchi K, Bolliger M, Blaiss C, Brose N, Liu X, Südhof T, Powell C. Increased anxiety‐like behavior in mice lacking the inhibitory synapse cell adhesion molecule neuroligin 2. Genes Brain & Behavior 2009, 8: 114-126. PMID: 19016888, PMCID: PMC2648807, DOI: 10.1111/j.1601-183x.2008.00455.x.
- Identification of Endogenous/transfected Synaptic Proteins in Primary Neuronal Culture by a High-yield Immunogold LabelingLiu X, Han W, Biederer T, Kavalali E, Südhof T. Identification of Endogenous/transfected Synaptic Proteins in Primary Neuronal Culture by a High-yield Immunogold Labeling. Microscopy And Microanalysis 2003, 9: 1498-1499. DOI: 10.1017/s1431927603447491.
- Synaptotagmin VII as a Plasma Membrane Ca2+ Sensor in ExocytosisSugita S, Han W, Butz S, Liu X, Fernández-Chacón R, Lao Y, Südhof T. Synaptotagmin VII as a Plasma Membrane Ca2+ Sensor in Exocytosis. Neuron 2001, 30: 459-473. PMID: 11395007, DOI: 10.1016/s0896-6273(01)00290-2.