Marcello DiStasio, MD, PhD
Research & Publications
Biography
News
Research Summary
Lab website: [distasiolab.org]
What role does inflammation play in the development and progression of degenerative diseases of the brain (such as Alzheimer’s disease) and retina (such as macular degeneration)? Activation of the innate immune system of the brain has been recently recognized as a significant component of numerous forms of neurodegenerative disease. My research aims to characterize the common pathways by which microglia, the primary phagocytic cells (consumers of debris) of central nervous system tissues, including the retina, switch from homeostatic (maintenance) to proinflammatory functions as part of disease progression. Using novel methods for single-cell RNA sequencing analysis (which characterizes the functional states of numerous cells at once) and multiplexed sequencing and proteomics in place on tissue (which allows us to visualize the location in tissues of many molecules, including RNA and protein), this work will identify immune system signaling pathways that are dysfunctional in degenerative disease. We hypothesize that there is a convergence of immune signaling pathways, meaning that the cells responsible for disruption of the normal processes that prevent damage to neurons become dysfunctional in stereotypical ways. This offers an opportunity for targeting these pathways using existing anti-inflammatory therapies. Thus, the goal is to characterize these pathways directly using human tissue samples and find key regulators of the transition from maintenance to inflammatory functions.
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Selected Publications
- Beyond the Lab and Into the Hospital: An Outlook on the Clinical Utility of Spatial Omics TechnologiesPucciarelli D, Lu B, Zlobec I, DiStasio M. Beyond the Lab and Into the Hospital: An Outlook on the Clinical Utility of Spatial Omics Technologies. GEN Biotechnology 2023, 2: 360-371. DOI: 10.1089/genbio.2023.0030.
- BSBM-18 SINGLE-CELL PROFILING TUMOR-INFILTRATING IMMUNE CELLS REVEALS CXCL13+ FOLLICULAR HELPER-LIKE CD4+ T CELLS IN HUMAN BRAIN TUMORSLu B, Lucca L, DiStasio M, Liu Y, Pham G, Buitrago-Pocasangre N, Arnal-Estape A, Moliterno J, Chiang V, Omuro A, Hafler D. BSBM-18 SINGLE-CELL PROFILING TUMOR-INFILTRATING IMMUNE CELLS REVEALS CXCL13+ FOLLICULAR HELPER-LIKE CD4+ T CELLS IN HUMAN BRAIN TUMORS. Neuro-Oncology Advances 2023, 5: iii4-iii4. PMCID: PMC10402449, DOI: 10.1093/noajnl/vdad070.014.
- Sporadic pituitary adenoma with somatic double-hit loss of MEN1Hong C, Alanya H, DiStasio M, Boulware S, Rimmer R, Omay S, Erson-Omay E. Sporadic pituitary adenoma with somatic double-hit loss of MEN1. Pituitary 2023, 26: 488-494. PMID: 37438451, DOI: 10.1007/s11102-023-01336-1.
- Single-cell analysis reveals inflammatory interactions driving macular degenerationKuchroo M, DiStasio M, Song E, Calapkulu E, Zhang L, Ige M, Sheth A, Majdoubi A, Menon M, Tong A, Godavarthi A, Xing Y, Gigante S, Steach H, Huang J, Huguet G, Narain J, You K, Mourgkos G, Dhodapkar R, Hirn M, Rieck B, Wolf G, Krishnaswamy S, Hafler B. Single-cell analysis reveals inflammatory interactions driving macular degeneration. Nature Communications 2023, 14: 2589. PMID: 37147305, PMCID: PMC10162998, DOI: 10.1038/s41467-023-37025-7.
- Neuropathology of New-Onset Refractory Status Epilepticus (NORSE)Hanin A, Cespedes J, Huttner A, Strelnikov D, Gopaul M, DiStasio M, Vezzani A, Hirsch L, Aronica E. Neuropathology of New-Onset Refractory Status Epilepticus (NORSE). Journal Of Neurology 2023, 270: 3688-3702. PMID: 37079033, DOI: 10.1007/s00415-023-11726-x.
- High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seqLiu Y, DiStasio M, Su G, Asashima H, Enninful A, Qin X, Deng Y, Nam J, Gao F, Bordignon P, Cassano M, Tomayko M, Xu M, Halene S, Craft J, Hafler D, Fan R. High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq. Nature Biotechnology 2023, 41: 1405-1409. PMID: 36823353, PMCID: PMC10567548, DOI: 10.1038/s41587-023-01676-0.
- Atypical thymomas with squamoid and spindle cell features: clinicopathologic, immunohistochemical and molecular genetic study of 120 cases with long-term follow-upSuster DI, Craig Mackinnon A, DiStasio M, Basu MK, Pihan G, Suster S. Atypical thymomas with squamoid and spindle cell features: clinicopathologic, immunohistochemical and molecular genetic study of 120 cases with long-term follow-up. Modern Pathology 2022, 35: 875-894. PMID: 35145198, DOI: 10.1038/s41379-022-01013-x.
- T lymphocytes and cytotoxic astrocyte blebs correlate across autism brainsDiStasio MM, Nagakura I, Nadler MJ, Anderson MP. T lymphocytes and cytotoxic astrocyte blebs correlate across autism brains. Annals Of Neurology 2019, 86: 885-898. PMID: 31591744, PMCID: PMC7210715, DOI: 10.1002/ana.25610.
- Correction: Use of Frontal Lobe Hemodynamics as Reinforcement Signals to an Adaptive ControllerDiStasio M, Francis J. Correction: Use of Frontal Lobe Hemodynamics as Reinforcement Signals to an Adaptive Controller. PLOS ONE 2013, 8: 10.1371/annotation/d72e0974-f492-4fa4-a850-6dd0017395b5. PMCID: PMC3859195, DOI: 10.1371/annotation/d72e0974-f492-4fa4-a850-6dd0017395b5.
- Sparse Coding of Movement-Related Neural ActivityDiStasio M, Chhatbar P, Francis J. Sparse Coding of Movement-Related Neural Activity. 2011, 1-5. DOI: 10.1109/spmb.2011.6120134.
- Effects of Working Memory Load on Oscillatory Power in Human Intracranial EEGMeltzer JA, Zaveri HP, Goncharova II, Distasio MM, Papademetris X, Spencer SS, Spencer DD, Constable RT. Effects of Working Memory Load on Oscillatory Power in Human Intracranial EEG. Cerebral Cortex 2007, 18: 1843-1855. PMID: 18056698, PMCID: PMC2474453, DOI: 10.1093/cercor/bhm213.
- The BioImage Suite Datatree Tool: Enabling Flexible Realtime Surgical VisualizationsDiStasio M, Vives K, Papademetris X. The BioImage Suite Datatree Tool: Enabling Flexible Realtime Surgical Visualizations. Insight Journal 2006 DOI: 10.54294/f0fw56.
- Development of a Research Interface for Image Guided Intervention: Initial Application to Epilepsy NeurosurgeryPapademetris X, Vives K, DiStasio M, Staib L, Neff M, Flossman S, Frielinghaus N, Zaveri H, Novotny E, Blumenfeld H, Constable R, Hetherington H, Duckrow R, Spencer S, Spencer D, Duncan J. Development of a Research Interface for Image Guided Intervention: Initial Application to Epilepsy Neurosurgery. 2006, 490-493. DOI: 10.1109/isbi.2006.1624960.