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Choukri Ben Mamoun, PhD

Professor of Medicine (Infectious Diseases) and of Microbial Pathogenesis; Affiliated Faculty, Yale Institute for Global Health

Research & Publications
Biography
News

Research Summary

Basic Research
Current research topics in the lab include:

- Identifying the molecular determinants of virulence of Babesia duncani and Babesia microti. We use mouse crosses, controlled infection and whole genome sequencing to identify specific host factors that play critical roles in Babesia virulence and pathogenesis.

- Understanding how Babesia parasites residing within human red blood cells deliver their secreted proteins to the mammalian host and how these proteins interact with the immune system of the host. Our lab discovered two main mechanisms by which Babesia parasites secrete their proteins: vesicular and non-vesicular mediated protein secretion. Our current efforts aim to identify the molecular signatures and machineries employed by these parasites to achieve successful delivery of their antigens into the host.

- Developing molecular tools for large scale functional analysis of parasite genomes.

- Using yeast as a model system to study the function and druggabality of pathogen targets.

Translational Medicine Program

Our translational medicine program builds upon the findings of our basic research efforts. Current investigations include:

- Antigen discovery and vaccine development with a specific focus on the human pathogens Babesia microti and Babesia duncani.

- Development of novel classes of antibabesial, antimalarial and antifungal drugs that target unique metabolic pathways essential for pathogen survival and virulence.

- Development of diagnostic assays for detection of active parasite infections.

Coauthors

Research Interests

Antifungal Agents; Babesiosis; Malaria; Opportunistic Infections; Protozoan Infections; Diagnostic Techniques and Procedures; Infectious Disease Medicine; Drug Discovery; Translational Research, Biomedical

Research Image

Malaria parasite in a human red blood cell

The image shows the final developmental stage (schizont) of the human malaria parasite Plasmodium falciparum during its intra-erythrocytic life cycle in human red blood cells.

Selected Publications

Epitope profiling of monoclonal antibodies to the immunodominant antigen BmGPI12 of the human pathogen Babesia microtiChand M, Choi J, Pal A, Singh P, Kumari V, Thekkiniath J, Gagnon J, Timalsina S, Gaur G, Williams S, Ledizet M, Mamoun C. Epitope profiling of monoclonal antibodies to the immunodominant antigen BmGPI12 of the human pathogen Babesia microti Frontiers In Cellular And Infection Microbiology 2022, 12: 1039197. DOI: 10.3389/fcimb.2022.1039197.
Epitope profiling of monoclonal antibodies to the immunodominant antigen BmGPI12 of the human pathogen Babesia microtiChand M, Choi J, Pal A, Singh P, Kumari V, Thekkiniath J, Gagnon J, Timalsina S, Gaur G, Williams S, Ledizet M, Mamoun C. Epitope profiling of monoclonal antibodies to the immunodominant antigen BmGPI12 of the human pathogen Babesia microti Frontiers In Cellular And Infection Microbiology 2022, 12: 1039197. PMID: 36506011, PMCID: PMC9732259, DOI: 10.3389/fcimb.2022.1039197.
Babesia duncani in Culture and in Mouse (ICIM) Model for the Advancement of Babesia Biology, Pathogenesis, and Therapy.Kumari V, Pal A, Singh P, Mamoun C. Babesia duncani in Culture and in Mouse (ICIM) Model for the Advancement of Babesia Biology, Pathogenesis, and Therapy. Bio-protocol 2022, 12 PMID: 36620533, PMCID: PMC9795036, DOI: 10.21769/bioprotoc.4549.
High-resolution crystal structure and chemical screening reveal pantothenate kinase as a new target for antifungal developmentGihaz S, Gareiss P, Choi JY, Renard I, Pal AC, Surovsteva Y, Chiu JE, Thekkiniath J, Plummer M, Hungerford W, Montgomery ML, Hosford A, Adams EM, Lightfoot JD, Fox D, Ojo KK, Staker BL, Fuller K, Ben Mamoun C. High-resolution crystal structure and chemical screening reveal pantothenate kinase as a new target for antifungal development Structure 2022, 30: 1494-1507.e6. PMID: 36167065, DOI: 10.1016/j.str.2022.09.001.
Specific and Sensitive Diagnosis of Babesia microti Active Infection Using Monoclonal Antibodies to the Immunodominant Antigen BmGPI12Gagnon J, Timalsina S, Choi JY, Chand M, Singh P, Lamba P, Gaur G, Pal AC, Mootien S, Marcos LA, Mamoun C, Ledizet M. Specific and Sensitive Diagnosis of Babesia microti Active Infection Using Monoclonal Antibodies to the Immunodominant Antigen BmGPI12 Journal Of Clinical Microbiology 2022, 60: e00925-22. PMID: 36040206, PMCID: PMC9491189, DOI: 10.1128/jcm.00925-22.
An Alternative Culture Medium for Continuous In Vitro Propagation of the Human Pathogen Babesia duncani in Human ErythrocytesSingh P, Pal AC, Mamoun CB. An Alternative Culture Medium for Continuous In Vitro Propagation of the Human Pathogen Babesia duncani in Human Erythrocytes Pathogens 2022, 11: 599. PMID: 35631120, PMCID: PMC9146245, DOI: 10.3390/pathogens11050599.
Targeting Pantothenate Kinase as an Effective Strategy for Antifungal Drug DevelopmentGihaz S, Gareiss P, Choi J, Renard I, Pal A, Surovsteva Y, Chiu J, Thekkiniath J, Plummer M, Hungerford W, Montgomery M, Hosford A, Adams E, Lightfoot J, Fox D, Ojo K, Staker B, Fuller K, Mamoun C. Targeting Pantothenate Kinase as an Effective Strategy for Antifungal Drug Development The FASEB Journal 2022, 36 DOI: 10.1096/fasebj.2022.36.s1.l7671.
Babesia duncani as a Model Organism to Study the Development, Virulence, and Drug Susceptibility of Intraerythrocytic Parasites In Vitro and In Vivo.Pal AC, Renard I, Singh P, Vydyam P, Chiu JE, Pou S, Winter RW, Dodean R, Frueh L, Nilsen AC, Riscoe MK, Doggett JS, Ben Mamoun C. Babesia duncani as a Model Organism to Study the Development, Virulence, and Drug Susceptibility of Intraerythrocytic Parasites In Vitro and In Vivo. The Journal Of Infectious Diseases 2022, 226: 1267-1275. PMID: 35512141, DOI: 10.1093/infdis/jiac181.
Redesigning therapies for pantothenate kinase–associated neurodegenerationMunshi MI, Yao SJ, Mamoun C. Redesigning therapies for pantothenate kinase–associated neurodegeneration Journal Of Biological Chemistry 2022, 298: 101577. PMID: 35041826, PMCID: PMC8861153, DOI: 10.1016/j.jbc.2022.101577.
Babesia microti Immunoreactive Rhoptry-Associated Protein-1 Paralogs Are Ancestral Members of the Piroplasmid-Confined RAP-1 FamilyBastos RG, Thekkiniath J, Mamoun C, Fuller L, Molestina RE, Florin-Christensen M, Schnittger L, Alzan HF, Suarez CE. Babesia microti Immunoreactive Rhoptry-Associated Protein-1 Paralogs Are Ancestral Members of the Piroplasmid-Confined RAP-1 Family Pathogens 2021, 10: 1384. PMID: 34832541, PMCID: PMC8624774, DOI: 10.3390/pathogens10111384.
BabesiosisChen T, Mamoun C, Krause P. Babesiosis 2021, 1298-1304. DOI: 10.1093/med/9780190888367.003.0200.
Treatment of Human Babesiosis: Then and Now.Renard I, Ben Mamoun C. Treatment of Human Babesiosis: Then and Now. Pathogens (Basel, Switzerland) 2021, 10 PMID: 34578153, PMCID: PMC8469882, DOI: 10.3390/pathogens10091120.
Recent Progress in Lyme Disease and Remaining ChallengesBobe JR, Jutras BL, Horn EJ, Embers ME, Bailey A, Moritz RL, Zhang Y, Soloski MJ, Ostfeld RS, Marconi RT, Aucott J, Ma'ayan A, Keesing F, Lewis K, Mamoun C, Rebman AW, McClune ME, Breitschwerdt EB, Reddy PJ, Maggi R, Yang F, Nemser B, Ozcan A, Garner O, Di Carlo D, Ballard Z, Joung HA, Garcia-Romeu A, Griffiths RR, Baumgarth N, Fallon BA. Recent Progress in Lyme Disease and Remaining Challenges Frontiers In Medicine 2021, 8: 666554. PMID: 34485323, PMCID: PMC8416313, DOI: 10.3389/fmed.2021.666554.
Effective Therapy Targeting Cytochrome bc1 Prevents Babesia Erythrocytic Development and Protects from Lethal InfectionChiu JE, Renard I, Pal AC, Singh P, Vydyam P, Thekkiniath J, Kumar M, Gihaz S, Pou S, Winter RW, Dodean R, Frueh L, Nilsen AC, Riscoe MK, Doggett JS, Mamoun C. Effective Therapy Targeting Cytochrome bc1 Prevents Babesia Erythrocytic Development and Protects from Lethal Infection Antimicrobial Agents And Chemotherapy 2021, 65: e00662-21. PMID: 34152821, PMCID: PMC8370247, DOI: 10.1128/aac.00662-21.
Cytochrome b Drug Resistance Mutation Decreases Babesia Fitness in the Tick Stages But Not the Mammalian Erythrocytic Cycle.Chiu JE, Renard I, George S, Pal A, Alday PH, Narasimhan S, Riscoe MK, Doggett JS, Ben Mamoun C. Cytochrome b Drug Resistance Mutation Decreases Babesia Fitness in the Tick Stages But Not the Mammalian Erythrocytic Cycle. The Journal Of Infectious Diseases 2021, 225: 135-145. PMID: 34139755, PMCID: PMC8730496, DOI: 10.1093/infdis/jiab321.
Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infectionOliva Chávez AS, Wang X, Marnin L, Archer NK, Hammond HL, Carroll EEM, Shaw DK, Tully BG, Buskirk AD, Ford SL, Butler LR, Shahi P, Morozova K, Clement CC, Lawres L, Neal A, Mamoun CB, Mason KL, Hobbs BE, Scoles GA, Barry EM, Sonenshine DE, Pal U, Valenzuela JG, Sztein MB, Pasetti MF, Levin ML, Kotsyfakis M, Jay SM, Huntley JF, Miller LS, Santambrogio L, Pedra JHF. Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection Nature Communications 2021, 12: 3696. PMID: 34140472, PMCID: PMC8211691, DOI: 10.1038/s41467-021-23900-8.
Anti-PfGARP activates programmed cell death of parasites and reduces severe malaria.Raj DK, Das Mohapatra A, Jnawali A, Zuromski J, Jha A, Cham-Kpu G, Sherman B, Rudlaff RM, Nixon CE, Hilton N, Oleinikov AV, Chesnokov O, Merritt J, Pond-Tor S, Burns L, Jolly G, Ben Mamoun C, Kabyemela E, Muehlenbachs A, Lambert L, Orr-Gonzalez S, Gnädig NF, Fidock DA, Park S, Dvorin JD, Pardi N, Weissman D, Mui BL, Tam YK, Friedman JF, Fried M, Duffy PE, Kurtis JD. Anti-PfGARP activates programmed cell death of parasites and reduces severe malaria. Nature 2020, 582: 104-108. PMID: 32427965, PMCID: PMC7372601, DOI: 10.1038/s41586-020-2220-1.
An improved and highly selective fluorescence assay for measuring phosphatidylserine decarboxylase activity Fluorescence detection of PS decarboxylase activityChoi JY, Black R, Lee H, Di Giovanni J, Murphy RC, Ben Mamoun C, Voelker DR. An improved and highly selective fluorescence assay for measuring phosphatidylserine decarboxylase activity Fluorescence detection of PS decarboxylase activity Journal Of Biological Chemistry 2020, 295: 9211-9222. PMID: 32430397, PMCID: PMC7335775, DOI: 10.1074/jbc.ra120.013421.
Palmitoylated Proteins in Plasmodium falciparum‐Infected Erythrocytes: Investigation with Click Chemistry and Metabolic LabelingKilian N, Zhang Y, LaMonica L, Hooker G, Toomre D, Mamoun CB, Ernst AM. Palmitoylated Proteins in Plasmodium falciparum‐Infected Erythrocytes: Investigation with Click Chemistry and Metabolic Labeling BioEssays 2020, 42: e1900145. PMID: 32342554, DOI: 10.1002/bies.201900145.
Efficient Single-Gene and Gene Family Editing in the Apicomplexan Parasite Eimeria tenella Using CRISPR-Cas9Hu D, Tang X, Mamoun C, Wang C, Wang S, Gu X, Duan C, Zhang S, Suo J, Deng M, Yu Y, Suo X, Liu X. Efficient Single-Gene and Gene Family Editing in the Apicomplexan Parasite Eimeria tenella Using CRISPR-Cas9 Frontiers In Bioengineering And Biotechnology 2020, 8: 128. PMID: 32158750, PMCID: PMC7052334, DOI: 10.3389/fbioe.2020.00128.
Evidence for vesicle-mediated antigen export by the human pathogen Babesia microtiThekkiniath J, Kilian N, Lawres L, Gewirtz MA, Graham MM, Liu X, Ledizet M, Mamoun C. Evidence for vesicle-mediated antigen export by the human pathogen Babesia microti Life Science Alliance 2019, 2: e201900382. PMID: 31196872, PMCID: PMC6572159, DOI: 10.26508/lsa.201900382.
Parasite‐Derived Vesicular‐Mediated Protein Export by the Human Pathogen Babesia microtiMamoun C, Thekkiniath J, Kilian N, Lawres L, Gewirtz M, Abraham A, Graham M, Liu X, Ledizet M. Parasite‐Derived Vesicular‐Mediated Protein Export by the Human Pathogen Babesia microti The FASEB Journal 2019, 33: 649.2-649.2. DOI: 10.1096/fasebj.2019.33.1_supplement.649.2.
Comparative 3D genome organization in apicomplexan parasitesBunnik EM, Venkat A, Shao J, McGovern KE, Batugedara G, Worth D, Prudhomme J, Lapp SA, Andolina C, Ross LS, Lawres L, Brady D, Sinnis P, Nosten F, Fidock DA, Wilson EH, Tewari R, Galinski MR, Ben Mamoun C, Ay F, Le Roch KG. Comparative 3D genome organization in apicomplexan parasites Proceedings Of The National Academy Of Sciences Of The United States Of America 2019, 116: 3183-3192. PMID: 30723152, PMCID: PMC6386730, DOI: 10.1073/pnas.1810815116.
Establishment of a continuous in vitro culture of Babesia duncani in human erythrocytes reveals unusually high tolerance to recommended therapiesAbraham A, Brasov I, Thekkiniath J, Kilian N, Lawres L, Gao R, DeBus K, He L, Yu X, Zhu G, Graham MM, Liu X, Molestina R, Ben Mamoun C. Establishment of a continuous in vitro culture of Babesia duncani in human erythrocytes reveals unusually high tolerance to recommended therapies Journal Of Biological Chemistry 2018, 293: 19974-19981. PMID: 30463941, PMCID: PMC6311517, DOI: 10.1074/jbc.ac118.005771.
BmGPAC, an Antigen Capture Assay for Detection of Active Babesia microti InfectionThekkiniath J, Mootien S, Lawres L, Perrin BA, Gewirtz M, Krause PJ, Williams S, Doggett J, Ledizet M, Mamoun C. BmGPAC, an Antigen Capture Assay for Detection of Active Babesia microti Infection Journal Of Clinical Microbiology 2018, 56: e00067-18. PMID: 30093394, PMCID: PMC6156295, DOI: 10.1128/jcm.00067-18.
Insights into the evolution and drug susceptibility of Babesia duncani from the sequence of its mitochondrial and apicoplast genomesVirji AZ, Thekkiniath J, Ma W, Lawres L, Knight J, Swei A, Roch KL, Mamoun C. Insights into the evolution and drug susceptibility of Babesia duncani from the sequence of its mitochondrial and apicoplast genomes International Journal For Parasitology 2018, 49: 105-113. PMID: 30176236, PMCID: PMC6395566, DOI: 10.1016/j.ijpara.2018.05.008.
BabesiosisMamoun C, Allred D. Babesiosis 2018, 1-8. DOI: 10.1002/9780470015902.a0001945.pub2.
Case report of the patient source of the Babesia microti R1 reference strain and implications for travelersStahl P, Poinsignon Y, Pouedras P, Ciubotaru V, Berry L, Emu B, Krause PJ, Mamoun C, Cornillot E. Case report of the patient source of the Babesia microti R1 reference strain and implications for travelers Journal Of Travel Medicine 2017, 25: tax073. PMID: 29394381, PMCID: PMC6927858, DOI: 10.1093/jtm/tax073.
A complex stereochemical relay approach to the antimalarial alkaloid ocimicide A 1 . Evidence for a structural revisionNikolayevskiy H, Tun M, Rablen PR, Mamoun C, Herzon SB. A complex stereochemical relay approach to the antimalarial alkaloid ocimicide A 1 . Evidence for a structural revision Chemical Science 2017, 8: 4867-4871. PMID: 28959410, PMCID: PMC5603897, DOI: 10.1039/c7sc01127j.
Antimalarial Properties of Simplified Kalihinol AnaloguesDaub ME, Prudhomme J, Mamoun C, Le Roch KG, Vanderwal CD. Antimalarial Properties of Simplified Kalihinol Analogues ACS Medicinal Chemistry Letters 2017, 8: 355-360. PMID: 28337330, PMCID: PMC5346982, DOI: 10.1021/acsmedchemlett.7b00013.
Genetic Evidence for Cytochrome b Qi Site Inhibition by 4(1H)-Quinolone-3-Diarylethers and Antimycin in Toxoplasma gondiiAlday PH, Bruzual I, Nilsen A, Pou S, Winter R, Mamoun C, Riscoe MK, Doggett JS. Genetic Evidence for Cytochrome b Qi Site Inhibition by 4(1H)-Quinolone-3-Diarylethers and Antimycin in Toxoplasma gondii Antimicrobial Agents And Chemotherapy 2017, 61: e01866-16. PMID: 27919897, PMCID: PMC5278733, DOI: 10.1128/aac.01866-16.
Open Source Drug Discovery with the Malaria Box Compound Collection for Neglected Diseases and BeyondVan Voorhis WC, Adams JH, Adelfio R, Ahyong V, Akabas MH, Alano P, Alday A, Resto Y, Alsibaee A, Alzualde A, Andrews KT, Avery SV, Avery VM, Ayong L, Baker M, Baker S, Mamoun C, Bhatia S, Bickle Q, Bounaadja L, Bowling T, Bosch J, Boucher LE, Boyom FF, Brea J, Brennan M, Burton A, Caffrey CR, Camarda G, Carrasquilla M, Carter D, Cassera M, Cheng K, Chindaudomsate W, Chubb A, Colon BL, Colón-López DD, Corbett Y, Crowther GJ, Cowan N, D’Alessandro S, Le Dang N, Delves M, DeRisi JL, Du AY, Duffy S, El-Sayed S, Ferdig MT, Robledo J, Fidock DA, Florent I, Fokou PV, Galstian A, Gamo FJ, Gokool S, Gold B, Golub T, Goldgof GM, Guha R, Guiguemde WA, Gural N, Guy RK, Hansen MA, Hanson KK, Hemphill A, van Huijsduijnen R, Horii T, Horrocks P, Hughes TB, Huston C, Igarashi I, Ingram-Sieber K, Itoe MA, Jadhav A, Jensen A, Jensen LT, Jiang RH, Kaiser A, Keiser J, Ketas T, Kicka S, Kim S, Kirk K, Kumar VP, Kyle DE, Lafuente MJ, Landfear S, Lee N, Lee S, Lehane AM, Li F, Little D, Liu L, Llinás M, Loza MI, Lubar A, Lucantoni L, Lucet I, Maes L, Mancama D, Mansour NR, March S, McGowan S, Vera I, Meister S, Mercer L, Mestres J, Mfopa AN, Misra RN, Moon S, Moore JP, da Costa F, Müller J, Muriana A, Hewitt S, Nare B, Nathan C, Narraidoo N, Nawaratna S, Ojo KK, Ortiz D, Panic G, Papadatos G, Parapini S, Patra K, Pham N, Prats S, Plouffe DM, Poulsen SA, Pradhan A, Quevedo C, Quinn RJ, Rice CA, Rizk M, Ruecker A, St. Onge R, Ferreira R, Samra J, Robinett NG, Schlecht U, Schmitt M, Villela F, Silvestrini F, Sinden R, Smith DA, Soldati T, Spitzmüller A, Stamm SM, Sullivan DJ, Sullivan W, Suresh S, Suzuki BM, Suzuki Y, Swamidass SJ, Taramelli D, Tchokouaha LR, Theron A, Thomas D, Tonissen KF, Townson S, Tripathi AK, Trofimov V, Udenze KO, Ullah I, Vallieres C, Vigil E, Vinetz JM, Vinh P, Vu H, Watanabe NA, Weatherby K, White PM, Wilks AF, Winzeler EA, Wojcik E, Wree M, Wu W, Yokoyama N, Zollo PH, Abla N, Blasco B, Burrows J, Laleu B, Leroy D, Spangenberg T, Wells T, Willis PA. Open Source Drug Discovery with the Malaria Box Compound Collection for Neglected Diseases and Beyond PLOS Pathogens 2016, 12: e1005763. PMID: 27467575, PMCID: PMC4965013, DOI: 10.1371/journal.ppat.1005763.
Radical cure of experimental babesiosis in immunodeficient mice using a combination of an endochin-like quinolone and atovaquoneLawres LA, Garg A, Kumar V, Bruzual I, Forquer IP, Renard I, Virji AZ, Boulard P, Rodriguez EX, Allen AJ, Pou S, Wegmann KW, Winter RW, Nilsen A, Mao J, Preston DA, Belperron AA, Bockenstedt LK, Hinrichs DJ, Riscoe MK, Doggett JS, Mamoun C. Radical cure of experimental babesiosis in immunodeficient mice using a combination of an endochin-like quinolone and atovaquone Journal Of Experimental Medicine 2016, 213: 1307-1318. PMID: 27270894, PMCID: PMC4925016, DOI: 10.1084/jem.20151519.
Targeting protein translation, RNA splicing, and degradation by morpholino-based conjugates in Plasmodium falciparumGarg A, Wesolowski D, Alonso D, Deitsch KW, Mamoun C, Altman S. Targeting protein translation, RNA splicing, and degradation by morpholino-based conjugates in Plasmodium falciparum Proceedings Of The National Academy Of Sciences Of The United States Of America 2015, 112: 11935-11940. PMID: 26351679, PMCID: PMC4586866, DOI: 10.1073/pnas.1515864112.
BabesiosisVannier EG, Diuk-Wasser MA, Mamoun C, Krause PJ. Babesiosis Infectious Disease Clinics Of North America 2015, 29: 357-370. PMID: 25999229, PMCID: PMC4458703, DOI: 10.1016/j.idc.2015.02.008.
Human babesiosisChen T, Mamoun C, Krause P. Human babesiosis 2015, 1295-1301. DOI: 10.1017/cbo9781139855952.226.
Functional analysis of Plasmodium falciparum genome using morpholino‐based toolsGarg A, Kumar V, Wesolowski D, Altman S, Mamoun C. Functional analysis of Plasmodium falciparum genome using morpholino‐based tools The FASEB Journal 2015, 29 DOI: 10.1096/fasebj.29.1_supplement.723.12.
Targeting Plasmodium falciparum Phosphethanolamine Methyltransferase for Malaria TherapyMamoun C, Kumar V. Targeting Plasmodium falciparum Phosphethanolamine Methyltransferase for Malaria Therapy The FASEB Journal 2015, 29 DOI: 10.1096/fasebj.29.1_supplement.885.25.
Structure, Function and Inhibition of the Phosphoethanolamine Methyltransferases of the Human Malaria Parasites Plasmodium vivax and Plasmodium knowlesiGarg A, Lukk T, Kumar V, Choi JY, Augagneur Y, Voelker DR, Nair S, Mamoun C. Structure, Function and Inhibition of the Phosphoethanolamine Methyltransferases of the Human Malaria Parasites Plasmodium vivax and Plasmodium knowlesi Scientific Reports 2015, 5: 9064. PMID: 25761669, PMCID: PMC4357015, DOI: 10.1038/srep09064.
From Protease to Decarboxylase THE MOLECULAR METAMORPHOSIS OF PHOSPHATIDYLSERINE DECARBOXYLASE*Choi JY, Duraisingh MT, Marti M, Ben Mamoun C, Voelker DR. From Protease to Decarboxylase THE MOLECULAR METAMORPHOSIS OF PHOSPHATIDYLSERINE DECARBOXYLASE* Journal Of Biological Chemistry 2015, 290: 10972-10980. PMID: 25724650, PMCID: PMC4409258, DOI: 10.1074/jbc.m115.642413.
Sequence and Annotation of the Apicoplast Genome of the Human Pathogen Babesia microtiGarg A, Stein A, Zhao W, Dwivedi A, Frutos R, Cornillot E, Mamoun C. Sequence and Annotation of the Apicoplast Genome of the Human Pathogen Babesia microti PLOS ONE 2014, 9: e107939. PMID: 25280009, PMCID: PMC4184790, DOI: 10.1371/journal.pone.0107939.
Plasmodium yoelii Vitamin B5 Pantothenate Transporter Candidate is Essential for Parasite Transmission to the MosquitoHart RJ, Lawres L, Fritzen E, Mamoun C, Aly AS. Plasmodium yoelii Vitamin B5 Pantothenate Transporter Candidate is Essential for Parasite Transmission to the Mosquito Scientific Reports 2014, 4: 5665. PMID: 25012929, PMCID: PMC4092334, DOI: 10.1038/srep05665.
Plasmodium falciparum phosphoethanolamine methyltransferase is essential for malaria transmissionBobenchik AM, Witola WH, Augagneur Y, Lochlainn L, Garg A, Pachikara N, Choi JY, Zhao YO, Usmani-Brown S, Lee A, Adjalley SH, Samanta S, Fidock DA, Voelker DR, Fikrig E, Mamoun C. Plasmodium falciparum phosphoethanolamine methyltransferase is essential for malaria transmission Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 18262-18267. PMID: 24145416, PMCID: PMC3831454, DOI: 10.1073/pnas.1313965110.
Quantitative PCR for Detection of Babesia microti in Ixodes scapularis Ticks and in Human BloodRollend L, Bent SJ, Krause PJ, Usmani-Brown S, Steeves TK, States SL, Lepore T, Ryan R, Dias F, Mamoun C, Fish D, Diuk-Wasser MA. Quantitative PCR for Detection of Babesia microti in Ixodes scapularis Ticks and in Human Blood Vector-Borne And Zoonotic Diseases 2013, 13: 784-790. PMID: 24107203, PMCID: PMC3822370, DOI: 10.1089/vbz.2011.0935.
Identification and Functional Analysis of the Primary Pantothenate Transporter, PfPAT, of the Human Malaria Parasite Plasmodium falciparum *Augagneur Y, Jaubert L, Schiavoni M, Pachikara N, Garg A, Usmani-Brown S, Wesolowski D, Zeller S, Ghosal A, Cornillot E, Said HM, Kumar P, Altman S, Mamoun C. Identification and Functional Analysis of the Primary Pantothenate Transporter, PfPAT, of the Human Malaria Parasite Plasmodium falciparum * Journal Of Biological Chemistry 2013, 288: 20558-20567. PMID: 23729665, PMCID: PMC3711320, DOI: 10.1074/jbc.m113.482992.
Plasmodium falciparum-Derived Uric Acid Precipitates Induce Maturation of Dendritic Cellsvan de Hoef DL, Coppens I, Holowka T, Mamoun C, Branch O, Rodriguez A. Plasmodium falciparum-Derived Uric Acid Precipitates Induce Maturation of Dendritic Cells PLOS ONE 2013, 8: e55584. PMID: 23405174, PMCID: PMC3565962, DOI: 10.1371/journal.pone.0055584.
Human CRM1 Augments Production of Infectious Human and Feline Immunodeficiency Viruses from Murine CellsElinav H, Wu Y, Coskun A, Hryckiewicz K, Kemler I, Hu Y, Rogers H, Hao B, Mamoun C, Poeschla E, Sutton R. Human CRM1 Augments Production of Infectious Human and Feline Immunodeficiency Viruses from Murine Cells Journal Of Virology 2012, 86: 12053-12068. PMID: 22933280, PMCID: PMC3486471, DOI: 10.1128/jvi.01970-12.
Sequencing of the smallest Apicomplexan genome from the human pathogen Babesia microtiCornillot E, Hadj-Kaddour K, Dassouli A, Noel B, Ranwez V, Vacherie B, Augagneur Y, Brès V, Duclos A, Randazzo S, Carcy B, Debierre-Grockiego F, Delbecq S, Moubri-Ménage K, Shams-Eldin H, Usmani-Brown S, Bringaud F, Wincker P, Vivarès CP, Schwarz RT, Schetters TP, Krause PJ, Gorenflot A, Berry V, Barbe V, Mamoun C. Sequencing of the smallest Apicomplexan genome from the human pathogen Babesia microti Nucleic Acids Research 2012, 40: 9102-9114. PMID: 22833609, PMCID: PMC3467087, DOI: 10.1093/nar/gks700.
Gene selective mRNA cleavage inhibits the development of Plasmodium falciparumAugagneur Y, Wesolowski D, Tae HS, Altman S, Mamoun C. Gene selective mRNA cleavage inhibits the development of Plasmodium falciparum Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 6235-6240. PMID: 22474358, PMCID: PMC3341008, DOI: 10.1073/pnas.1203516109.
Identification of Gene Encoding Plasmodium knowlesi Phosphatidylserine Decarboxylase by Genetic Complementation in Yeast and Characterization of in Vitro Maturation of Encoded Enzyme*Choi JY, Augagneur Y, Mamoun C, Voelker DR. Identification of Gene Encoding Plasmodium knowlesi Phosphatidylserine Decarboxylase by Genetic Complementation in Yeast and Characterization of in Vitro Maturation of Encoded Enzyme* Journal Of Biological Chemistry 2011, 287: 222-232. PMID: 22057268, PMCID: PMC3249073, DOI: 10.1074/jbc.m111.313676.
Phosphoethanolamine methyltransferases in phosphocholine biosynthesis: functions and potential for antiparasite therapyBobenchik AM, Augagneur Y, Hao B, Hoch JC, Mamoun C. Phosphoethanolamine methyltransferases in phosphocholine biosynthesis: functions and potential for antiparasite therapy FEMS Microbiology Reviews 2011, 35: 609-619. PMID: 21303393, PMCID: PMC4107886, DOI: 10.1111/j.1574-6976.2011.00267.x.
PfNT2, a Permease of the Equilibrative Nucleoside Transporter Family in the Endoplasmic Reticulum of Plasmodium falciparum *Downie MJ, Bissati K, Bobenchik AM, Lochlainn L, Amerik A, Zufferey R, Kirk K, Mamoun C. PfNT2, a Permease of the Equilibrative Nucleoside Transporter Family in the Endoplasmic Reticulum of Plasmodium falciparum * Journal Of Biological Chemistry 2010, 285: 20827-20833. PMID: 20439460, PMCID: PMC2898299, DOI: 10.1074/jbc.m110.118489.
Targeting the lipid metabolic pathways for the treatment of malariaMamoun C, Prigge ST, Vial H. Targeting the lipid metabolic pathways for the treatment of malaria Drug Development Research 2009, 71: 44-55. PMID: 20559451, PMCID: PMC2886290, DOI: 10.1002/ddr.20347.
Disruption of the Plasmodium falciparum PfPMT Gene Results in a Complete Loss of Phosphatidylcholine Biosynthesis via the Serine-Decarboxylase-Phosphoethanolamine-Methyltransferase Pathway and Severe Growth and Survival Defects*Witola WH, El Bissati K, Pessi G, Xie C, Roepe PD, Mamoun CB. Disruption of the Plasmodium falciparum PfPMT Gene Results in a Complete Loss of Phosphatidylcholine Biosynthesis via the Serine-Decarboxylase-Phosphoethanolamine-Methyltransferase Pathway and Severe Growth and Survival Defects* Journal Of Biological Chemistry 2008, 283: 27636-27643. PMID: 18694927, PMCID: PMC2562060, DOI: 10.1074/jbc.m804360200.
Genetic evidence for the essential role of PfNT1 in the transport and utilization of xanthine, guanine, guanosine and adenine by Plasmodium falciparumBissati K, Downie MJ, Kim SK, Horowitz M, Carter N, Ullman B, Mamoun C. Genetic evidence for the essential role of PfNT1 in the transport and utilization of xanthine, guanine, guanosine and adenine by Plasmodium falciparum Molecular And Biochemical Parasitology 2008, 161: 130-139. PMID: 18639591, PMCID: PMC2612043, DOI: 10.1016/j.molbiopara.2008.06.012.
Choline Induces Transcriptional Repression and Proteasomal Degradation of the Malarial Phosphoethanolamine Methyltransferase▿Witola WH, Mamoun C. Choline Induces Transcriptional Repression and Proteasomal Degradation of the Malarial Phosphoethanolamine Methyltransferase▿ MSphere 2007, 6: 1618-1624. PMID: 17644653, PMCID: PMC2043360, DOI: 10.1128/ec.00229-07.
Purification of Components of the Translation Elongation Factor Complex of Plasmodium falciparum by Tandem Affinity Purification▿Takebe S, Witola WH, Schimanski B, Günzl A, Mamoun C. Purification of Components of the Translation Elongation Factor Complex of Plasmodium falciparum by Tandem Affinity Purification▿ MSphere 2007, 6: 584-591. PMID: 17307963, PMCID: PMC1865644, DOI: 10.1128/ec.00376-06.
Amino acids mediate colony and cell differentiation in the fungal pathogen Candida parapsilosisKim SK, Bissati K, Mamoun C. Amino acids mediate colony and cell differentiation in the fungal pathogen Candida parapsilosis Microbiology 2006, 152: 2885-2894. PMID: 17005970, DOI: 10.1099/mic.0.29180-0.
The plasma membrane permease PfNT1 is essential for purine salvage in the human malaria parasite Plasmodium falciparumBissati K, Zufferey R, Witola WH, Carter NS, Ullman B, Mamoun C. The plasma membrane permease PfNT1 is essential for purine salvage in the human malaria parasite Plasmodium falciparum Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 9286-9291. PMID: 16751273, PMCID: PMC1482602, DOI: 10.1073/pnas.0602590103.
Pathways for phosphatidylcholine biosynthesis: targets and strategies for antimalarial drugsPessi G, Mamoun C. Pathways for phosphatidylcholine biosynthesis: targets and strategies for antimalarial drugs Clinical Lipidology And Metabolic Disorders 2006, 1: 173-180. DOI: 10.2217/17460875.1.2.173.
Leishmania major Expresses a Single Dihydroxyacetone Phosphate Acyltransferase Localized in the Glycosome, Important for Rapid Growth and Survival at High Cell Density and Essential for Virulence*Zufferey R, Mamoun C. Leishmania major Expresses a Single Dihydroxyacetone Phosphate Acyltransferase Localized in the Glycosome, Important for Rapid Growth and Survival at High Cell Density and Essential for Virulence* Journal Of Biological Chemistry 2006, 281: 7952-7959. PMID: 16423830, DOI: 10.1074/jbc.m512911200.
Plasmodium Lipids: Metabolism and FunctionVial H, Mamoun C. Plasmodium Lipids: Metabolism and Function 2005, 327-352. DOI: 10.1128/9781555817558.ch17.
Unraveling the Mode of Action of the Antimalarial Choline Analog G25 in Plasmodium falciparum and Saccharomyces cerevisiaeRoggero R, Zufferey R, Minca M, Richier E, Calas M, Vial H, Mamoun C. Unraveling the Mode of Action of the Antimalarial Choline Analog G25 in Plasmodium falciparum and Saccharomyces cerevisiae Antimicrobial Agents And Chemotherapy 2004, 48: 2816-2824. PMID: 15273086, PMCID: PMC478495, DOI: 10.1128/aac.48.8.2816-2824.2004.
A pathway for phosphatidylcholine biosynthesis in Plasmodium falciparum involving phosphoethanolamine methylationPessi G, Kociubinski G, Mamoun CB. A pathway for phosphatidylcholine biosynthesis in Plasmodium falciparum involving phosphoethanolamine methylation Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 6206-6211. PMID: 15073329, PMCID: PMC395947, DOI: 10.1073/pnas.0307742101.
Localization of the Plasmodium falciparumPfNT1 Nucleoside Transporter to the Parasite Plasma Membrane*Rager N, Mamoun C, Carter N, Goldberg D, Ullman B. Localization of the Plasmodium falciparumPfNT1 Nucleoside Transporter to the Parasite Plasma Membrane* Journal Of Biological Chemistry 2001, 276: 41095-41099. PMID: 11682491, DOI: 10.1074/jbc.m107037200.
Plasmodium protein phosphatase 2C dephosphorylates translation elongation factor 1β and inhibits its PKC‐mediated nucleotide exchange activity in vitroMamoun C, Goldberg D. Plasmodium protein phosphatase 2C dephosphorylates translation elongation factor 1β and inhibits its PKC‐mediated nucleotide exchange activity in vitro Molecular Microbiology 2001, 39: 973-981. PMID: 11251817, DOI: 10.1046/j.1365-2958.2001.02289.x.
Co‐ordinated programme of gene expression during asexual intraerythrocytic development of the human malaria parasite Plasmodium falciparum revealed by microarray analysisMamoun C, Gluzman I, Hott C, MacMillan S, Amarakone A, Anderson D, Carlton J, Dame J, Chakrabarti D, Martin R, Brownstein B, Goldberg D. Co‐ordinated programme of gene expression during asexual intraerythrocytic development of the human malaria parasite Plasmodium falciparum revealed by microarray analysis Molecular Microbiology 2001, 39: 26-36. PMID: 11123685, DOI: 10.1046/j.1365-2958.2001.02222.x.
Transposition of the Drosophila element mariner within the human malaria parasite Plasmodium falciparumMamoun C, Gluzman I, Beverley S, Goldberg D. Transposition of the Drosophila element mariner within the human malaria parasite Plasmodium falciparum Molecular And Biochemical Parasitology 2000, 110: 405-407. PMID: 11071293, DOI: 10.1016/s0166-6851(00)00268-1.
Isolation and Functional Characterization of the PfNT1 Nucleoside Transporter Gene from Plasmodium falciparum *Carter N, Mamoun C, Liu W, Silva E, Landfear S, Goldberg D, Ullman B. Isolation and Functional Characterization of the PfNT1 Nucleoside Transporter Gene from Plasmodium falciparum * Journal Of Biological Chemistry 2000, 275: 10683-10691. PMID: 10744765, DOI: 10.1074/jbc.275.14.10683.
Transfer of genes into Plasmodium falciparum by polyamidoamine dendrimersMamoun C, Truong R, Gluzman I, Akopyants N, Oksman A, Goldberg D. Transfer of genes into Plasmodium falciparum by polyamidoamine dendrimers Molecular And Biochemical Parasitology 1999, 103: 117-121. PMID: 10514088, DOI: 10.1016/s0166-6851(99)00116-4.
A set of independent selectable markers for transfection of the human malaria parasite Plasmodium falciparumMamoun C, Gluzman I, Goyard S, Beverley S, Goldberg D. A set of independent selectable markers for transfection of the human malaria parasite Plasmodium falciparum Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 8716-8720. PMID: 10411941, PMCID: PMC17582, DOI: 10.1073/pnas.96.15.8716.
Disruption of YHC8, a Member of the TSR1 Gene Family, Reveals Its Direct Involvement in Yeast Protein Translocation*Mamoun C, Beckerich J, Gaillardin C, Kepes F. Disruption of YHC8, a Member of the TSR1 Gene Family, Reveals Its Direct Involvement in Yeast Protein Translocation* Journal Of Biological Chemistry 1999, 274: 11296-11302. PMID: 10196219, DOI: 10.1074/jbc.274.16.11296.
Identification and Characterization of an Unusual Double Serine/Threonine Protein Phosphatase 2C in the Malaria ParasitePlasmodium falciparum *Mamoun C, Sullivan D, Banerjee R, Goldberg D. Identification and Characterization of an Unusual Double Serine/Threonine Protein Phosphatase 2C in the Malaria ParasitePlasmodium falciparum * Journal Of Biological Chemistry 1998, 273: 11241-11247. PMID: 9556615, DOI: 10.1074/jbc.273.18.11241.
Yarrowia lipolytica TSR1 Gene ProductMamoun C, Beckerich J, Gaillardin C. Yarrowia lipolytica TSR1 Gene Product Journal Of Biological Chemistry 1997, 272: 24594-24598. PMID: 9305926, DOI: 10.1074/jbc.272.39.24594.
The TSR1 Gene of Yarrowia lipolytica Is Involved in the Signal Recognition Particle-dependent Translocation Pathway of Secretory Proteins*Mamoun C, Beckerich J, Gaillardin C. The TSR1 Gene of Yarrowia lipolytica Is Involved in the Signal Recognition Particle-dependent Translocation Pathway of Secretory Proteins* Journal Of Biological Chemistry 1996, 271: 23895-23901. PMID: 8798620, DOI: 10.1074/jbc.271.39.23895.

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