2022
How to correct relative voxel scale factors for calculations of vector-difference Fourier maps in cryo-EM
Wang J, Liu J, Gisriel CJ, Wu S, Maschietto F, Flesher DA, Lolis E, Lisi GP, Brudvig GW, Xiong Y, Batista VS. How to correct relative voxel scale factors for calculations of vector-difference Fourier maps in cryo-EM. Journal Of Structural Biology 2022, 214: 107902. PMID: 36202310, PMCID: PMC10226527, DOI: 10.1016/j.jsb.2022.107902.Peer-Reviewed Original ResearchConceptsCryo-EM mapsAmino acid residuesAcid residuesCryo-electron microscopy mapIndividual amino acid residuesCyanobacteria Synechocystis spPCC 6803Synechocystis spMicroscopy mapsThermosynechococcus elongatusSARS-CoV-2 spike proteinLocal structural changesResiduesSpike proteinAtomic coordinatesElongatusSubunitsSpeciesProteinSpSimilar structureStructural changes
2019
Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome
Armache J, Gamarra N, Johnson S, Leonard J, Wu S, Narlikar G, Cheng Y. Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome. ELife 2019, 8: e46057. PMID: 31210637, PMCID: PMC6611695, DOI: 10.7554/elife.46057.Peer-Reviewed Original Research
2012
Fabs Enable Single Particle cryoEM Studies of Small Proteins
Wu S, Avila-Sakar A, Kim J, Booth D, Greenberg C, Rossi A, Liao M, Li X, Alian A, Griner S, Juge N, Yu Y, Mergel C, Chaparro-Riggers J, Strop P, Tampé R, Edwards R, Stroud R, Craik C, Cheng Y. Fabs Enable Single Particle cryoEM Studies of Small Proteins. Structure 2012, 20: 582-592. PMID: 22483106, PMCID: PMC3322386, DOI: 10.1016/j.str.2012.02.017.Peer-Reviewed Original ResearchCryoelectron MicroscopyEscherichia coliEscherichia coli ProteinsHumansImage Processing, Computer-AssistedImmunoglobulin Fab FragmentsModels, MolecularMolecular WeightPeptide LibraryProprotein Convertase 9Proprotein ConvertasesProtein ConformationRecombinant ProteinsSerine EndopeptidasesVesicular Glutamate Transport Proteins
2011
Self‐assembly of amelogenin proteins at the water–oil interface
Martinez‐Avila O, Wu S, Cheng Y, Lee R, Khan F, Habelitz S. Self‐assembly of amelogenin proteins at the water–oil interface. European Journal Of Oral Sciences 2011, 119: 75-82. PMID: 22243231, PMCID: PMC6339812, DOI: 10.1111/j.1600-0722.2011.00907.x.Peer-Reviewed Original ResearchConceptsSelf-assembled structuresWater-oil interfaceAtomic force microscopySubsequent fibril formationHelical nanostructuresDynamic lightReverse micellesOil emulsionsForce microscopyNanoribbon formationInitial pHElectron microscopyHydrophobic domainFibril formationNanoribbonsParallel alignmentAmelogenin cleavage productsSame conditionsEnamel biomineralizationMicroscopyHydrophilic C-terminusCleavage productsRh174FormationMicelles