2021
Deletion of Cdh16 Ksp-cadherin leads to a developmental delay in the ability to maximally concentrate urine in mouse
Thomson R, Dynia DW, Burlein S, Thomson BR, Booth C, Knauf F, Wang T, Aronson P. Deletion of Cdh16 Ksp-cadherin leads to a developmental delay in the ability to maximally concentrate urine in mouse. American Journal Of Physiology. Renal Physiology 2021, 320: f1106-f1122. PMID: 33938239, PMCID: PMC8285649, DOI: 10.1152/ajprenal.00556.2020.Peer-Reviewed Original ResearchConceptsKsp-cadherinCell adhesion moleculeAtypical memberKidney developmentMammalian kidneyAdult mammalian kidneyBasolateral membraneNormal kidney developmentEpithelial cellsAdhesion moleculesMutant animalsExpression analysisSpecific expressionE-cadherin expressionWestern blot analysisEpithelial phenotypePrincipal proteinE-cadherinBlot analysisMouse linesAquaporin-2CadherinCritical roleDevelopmental delayKnockout mice
2007
NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity
Kocinsky HS, Dynia DW, Wang T, Aronson PS. NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity. American Journal Of Physiology. Renal Physiology 2007, 293: f212-f218. PMID: 17409282, DOI: 10.1152/ajprenal.00042.2007.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthineAnimalsCells, CulturedColforsinCyclic AMP-Dependent Protein KinasesElectrophoresis, Polyacrylamide GelKidneyKidney Tubules, ProximalMaleMicrovilliParathyroid HormonePhosphodiesterase InhibitorsPhosphorylationRatsRats, Sprague-DawleySerineSodiumSodium RadioisotopesSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersStimulation, ChemicalConceptsSerine 552NHE3 phosphorylationNHE3 activityMicrovillar membrane vesiclesPhosphorylation of NHE3Direct phosphorylationPhosphospecific antibodiesSprague-Dawley ratsExchanger type 3PKA activationMembrane vesiclesSite-specific changesPhosphorylationTransport activityOpossum kidney cellsParathyroid hormoneIntravenous infusionRat modelExchange activityKidney cellsOKP cellsNHE3 inhibitionPKACell modelExact role
2005
Role of PDZK1 in membrane expression of renal brush border ion exchangers
Thomson RB, Wang T, Thomson BR, Tarrats L, Girardi A, Mentone S, Soleimani M, Kocher O, Aronson PS. Role of PDZK1 in membrane expression of renal brush border ion exchangers. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 13331-13336. PMID: 16141316, PMCID: PMC1201624, DOI: 10.1073/pnas.0506578102.Peer-Reviewed Original ResearchConceptsProximal tubulesExpression of NHE3Functional activityRole of PDZK1Protein PDZK1Reabsorption of NaBrush border expressionMammalian kidneyCFEXBrush border localizationMutant miceGST fusion proteinProtein expressionKidneyBrush border membrane proteinsExchanger NHE3Membrane expressionNHE3Brush borderBrush border membrane vesiclesPDZK1 interactionTubulesNormal expressionMembrane proteinsPDZK1
2004
NHE2‐mediated bicarbonate reabsorption in the distal tubule of NHE3 null mice
Bailey MA, Giebisch G, Abbiati T, Aronson PS, Gawenis LR, Shull GE, Wang T. NHE2‐mediated bicarbonate reabsorption in the distal tubule of NHE3 null mice. The Journal Of Physiology 2004, 561: 765-775. PMID: 15604231, PMCID: PMC1665379, DOI: 10.1113/jphysiol.2004.074716.Peer-Reviewed Original ResearchConceptsBicarbonate excretionDistal tubulesBicarbonate reabsorptionNHE3 null miceBicarbonate loadEarly distal tubule fluidNull miceNephron segmentsVivo micropunctureDistal tubule fluidProximal tubule bicarbonate reabsorptionRenal clearance experimentsUrinary bicarbonate excretionGlomerular filtration rateDistal nephron segmentsUrinary bicarbonate lossRat distal tubulesDistal bicarbonate reabsorptionExcretion of bicarbonateMetabolic acidosisFiltration rateVivo microperfusionClearance experimentsReabsorption capacityMicroperfusion experimentsRenal and intestinal transport defects in Slc26a6-null mice
Wang Z, Wang T, Petrovic S, Tuo B, Riederer B, Barone S, Lorenz JN, Seidler U, Aronson PS, Soleimani M. Renal and intestinal transport defects in Slc26a6-null mice. American Journal Of Physiology - Cell Physiology 2004, 288: c957-c965. PMID: 15574486, DOI: 10.1152/ajpcell.00505.2004.Peer-Reviewed Original ResearchConceptsWild-type miceProximal tubulesSlc26a6-null miceHCO3- secretionKidney proximal tubulesApical membrane ClNull miceBaseline rateNormal blood pressureCl-/formate exchangeBlood pressureKidney functionElectrolyte profileMucosal tissuesIntestinal physiologyUssing chambersSmall intestineMiceFluid absorptionNaCl absorptionStatistical significanceCl-/HCO3NaCl transportDuodenumTubules
2002
Formate-stimulated NaCl absorption in the proximal tubule is independent of the pendrin protein
Karniski LP, Wang T, Everett LA, Green ED, Giebisch G, Aronson PS. Formate-stimulated NaCl absorption in the proximal tubule is independent of the pendrin protein. American Journal Of Physiology. Renal Physiology 2002, 283: f952-f956. PMID: 12372770, DOI: 10.1152/ajprenal.00182.2002.Peer-Reviewed Original ResearchConceptsProximal tubulesChloride/formate exchangeNaCl reabsorptionChloride reabsorptionElectroneutral NaCl reabsorptionNaCl absorptionRole of pendrinFormate exchangePds-knockout mouseRecycling of formatePendrin proteinReabsorptionMicePendrinBrush border membrane vesiclesTubulesPresent studyChloride transportApical membraneUse of transgenic animals to study renal acid-base transport.
Wang T, Giebisch G, Aronson PS. Use of transgenic animals to study renal acid-base transport. Journal Of Nephrology 2002, 15 Suppl 5: s151-60. PMID: 12027214.Commentaries, Editorials and LettersConceptsAcid-base transportSpecific transporter isoformsSuch knockout miceRenal acid-base transportRenal acid-base homeostasisTransgenic miceTransporter isoformsPump subunitsTransgenic animalsKnockout micePhysiological roleATPase isoformsTransport deficiencyMolecular levelAcid-base homeostasisIsoformsAdaptive mechanismsCarbonic anhydraseCompensatory-adaptive mechanismsUseful experimental modelTransportersRegulatory mediatorsNHE isoformsNitric oxideExperimental model
2001
Role of NHE isoforms in mediating bicarbonate reabsorption along the nephron
Wang T, Hropot M, Aronson P, Giebisch G. Role of NHE isoforms in mediating bicarbonate reabsorption along the nephron. American Journal Of Physiology. Renal Physiology 2001, 281: f1117-f1122. PMID: 11704563, DOI: 10.1152/ajprenal.2001.281.6.f1117.Peer-Reviewed Original ResearchConceptsLoop of HenleHOE 694Proximal tubulesAddition of ethylisopropylamilorideDistal convoluted tubuleBicarbonate reabsorptionConvoluted tubulesSitu microperfusionRat kidneySignificant inhibitionNHE isoformsHenlePredominant isoformTubulesNHE3Functional roleInhibitionNHE2Apical membraneIsoformsApical isoformKidneyMicroperfusionEthylisopropylamilorideNephronEssential role of NHE3 in facilitating formate-dependent NaCl absorption in the proximal tubule
Wang T, Yang C, Abbiati T, Shull G, Giebisch G, Aronson P. Essential role of NHE3 in facilitating formate-dependent NaCl absorption in the proximal tubule. American Journal Of Physiology. Renal Physiology 2001, 281: f288-f292. PMID: 11457720, DOI: 10.1152/ajprenal.2001.281.2.f288.Peer-Reviewed Original Research
1999
Mechanism of proximal tubule bicarbonate absorption in NHE3 null mice
Wang T, Yang C, Abbiati T, Schultheis P, Shull G, Giebisch G, Aronson P. Mechanism of proximal tubule bicarbonate absorption in NHE3 null mice. American Journal Of Physiology 1999, 277: f298-f302. PMID: 10444585, DOI: 10.1152/ajprenal.1999.277.2.f298.Peer-Reviewed Original Research
1998
Renal and intestinal absorptive defects in mice lacking the NHE3 Na+/H+ exchanger
Schultheis P, Clarke L, Meneton P, Miller M, Soleimani M, Gawenis L, Riddle T, Duffy J, Doetschman T, Wang T, Giebisch G, Aronson P, Lorenz J, Shull G. Renal and intestinal absorptive defects in mice lacking the NHE3 Na+/H+ exchanger. Nature Genetics 1998, 19: 282-285. PMID: 9662405, DOI: 10.1038/969.Peer-Reviewed Original ResearchConceptsAcid-base balanceAbsorptive defectsNHE3-deficient miceFluid volume homeostasisRenal proximalSlight diarrheaPlasma aldosteroneBlood pressureHomozygous mutant miceExchanger mRNANHE2 isoformsBlood analysisMutant miceCompensatory mechanismsFluid absorptionNHE3 functionVolume homeostasisMiceDistribution of NHE3Cl-/HCO3Channel activityNHE3KidneyATPase mRNAIntestineEffect of metabolic acidosis on NaCl transport in the proximal tubule
Wang T, Egbert A, Aronson P, Giebisch G. Effect of metabolic acidosis on NaCl transport in the proximal tubule. American Journal Of Physiology 1998, 274: f1015-f1019. PMID: 9841491, DOI: 10.1152/ajprenal.1998.274.6.f1015.Peer-Reviewed Original ResearchConceptsMetabolic acidosisProximal tubulesNaCl reabsorptionRat proximal tubule segmentsAcidotic ratsNaCl transportNaCl absorptionLate proximal tubuleProximal tubule segmentsControl ratsCapillary perfusateBicarbonate absorptionNephron segmentsAcidosisTubule segmentsRatsRecent evidenceJclTubulesFormate exchangeReabsorptionDifferential regulation
1996
Mechanisms of stimulation of proximal tubule chloride transport by formate and oxalate
Wang T, Egbert AL, Abbiati T, Aronson PS, Giebisch G. Mechanisms of stimulation of proximal tubule chloride transport by formate and oxalate. American Journal Of Physiology 1996, 271: f446-f450. PMID: 8770178, DOI: 10.1152/ajprenal.1996.271.2.f446.Peer-Reviewed Original Research
1995
Stimulation of chloride transport by cAMP in rat proximal tubules
Wang T, Segal AS, Giebisch G, Aronson PS. Stimulation of chloride transport by cAMP in rat proximal tubules. American Journal Of Physiology 1995, 268: f204-f210. PMID: 7864157, DOI: 10.1152/ajprenal.1995.268.2.f204.Peer-Reviewed Original ResearchConceptsRat proximal tubuleProximal tubulesDibutyryl cAMPCapillary perfusateLate proximal tubuleProximal tubule cellsCl- channel blockerCl- channelsTranscellular Cl absorptionAddition of forskolinLuminal DIDSSensitive Cl- channelBasolateral membraneLuminal perfusateTubule cellsCAMP productionFluid absorptionCl absorptionMM ClPerfusateJclRecent evidenceLuminal membraneTubulesEffect of cAMP
1993
Effects of formate and oxalate on chloride absorption in rat distal tubule
Wang T, Agulian SK, Giebisch G, Aronson PS. Effects of formate and oxalate on chloride absorption in rat distal tubule. American Journal Of Physiology 1993, 264: f730-f736. PMID: 8386473, DOI: 10.1152/ajprenal.1993.264.4.f730.Peer-Reviewed Original Research
1992
Effects of formate and oxalate on volume absorption in rat proximal tubule
Wang T, Giebisch G, Aronson PS. Effects of formate and oxalate on volume absorption in rat proximal tubule. American Journal Of Physiology 1992, 263: f37-f42. PMID: 1636743, DOI: 10.1152/ajprenal.1992.263.1.f37.Peer-Reviewed Original ResearchConceptsLuminal perfusateProximal tubulesCapillary perfusateLate proximal tubuleRat proximal tubuleHCO3- Ringer's solutionPhysiological rangePeritubular capillariesChannel blockersStandard Ringer solutionPerfusateFluid absorptionStimulatory effectNaCl absorptionRinger's solutionTubulesEffect of formateBlockers