2015
bSUM: A bead-supported unilamellar membrane system facilitating unidirectional insertion of membrane proteins into giant vesicles
Zheng H, Lee S, Llaguno MC, Jiang QX. bSUM: A bead-supported unilamellar membrane system facilitating unidirectional insertion of membrane proteins into giant vesicles. The Journal Of General Physiology 2015, 147: 77-93. PMID: 26712851, PMCID: PMC4692488, DOI: 10.1085/jgp.201511448.Peer-Reviewed Original ResearchConceptsMembrane proteinsIon channelsLipid compositionLipid bilayersMembrane systemLipid-protein interactionsVitro membrane systemSingle-molecule imagingVoltage-gated ion channelsGiant vesiclesUnilamellar naturePlanar electrodesPlanar lipid bilayersSuch proteinsMicroscopic imagingBiophysical analysisFast gating kineticsUnidirectional insertionProteinChannel activityProtein orientationGating kineticsModel systemBilayersSpecific ligands
2012
Phase transitions in the assembly of multivalent signalling proteins
Li P, Banjade S, Cheng HC, Kim S, Chen B, Guo L, Llaguno M, Hollingsworth JV, King DS, Banani SF, Russo PS, Jiang QX, Nixon BT, Rosen MK. Phase transitions in the assembly of multivalent signalling proteins. Nature 2012, 483: 336-340. PMID: 22398450, PMCID: PMC3343696, DOI: 10.1038/nature10879.Peer-Reviewed Original ResearchMeSH KeywordsActin-Related Protein 2-3 ComplexAdaptor Proteins, Signal TransducingBinding SitesBiopolymersFluorescence Recovery After PhotobleachingHeLa CellsHumansLigandsMembrane ProteinsMultiprotein ComplexesOncogene ProteinsPhase TransitionPhosphorylationProline-Rich Protein DomainsProtein Structure, QuaternaryProteinsSignal TransductionSrc Homology DomainsWiskott-Aldrich Syndrome Protein, Neuronal