2014
MUT-14 and SMUT-1 DEAD Box RNA Helicases Have Overlapping Roles in Germline RNAi and Endogenous siRNA Formation
Phillips C, Montgomery B, Breen P, Roovers E, Rim Y, Ohsumi T, Newman M, van Wolfswinkel J, Ketting R, Ruvkun G, Montgomery T. MUT-14 and SMUT-1 DEAD Box RNA Helicases Have Overlapping Roles in Germline RNAi and Endogenous siRNA Formation. Current Biology 2014, 24: 839-844. PMID: 24684932, PMCID: PMC4010136, DOI: 10.1016/j.cub.2014.02.060.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCaenorhabditis elegansCaenorhabditis elegans ProteinsDEAD-box RNA HelicasesFluoroimmunoassayGerm CellsImmunoprecipitationMolecular Sequence DataReal-Time Polymerase Chain ReactionRNA InterferenceRNA, Small InterferingSaccharomyces cerevisiaeSequence AlignmentSequence Analysis, DNAConceptsSiRNA formationMutator fociClass genesDEAD-box RNA helicasesBox RNA helicasesElegans genesEndogenous siRNAsSiRNA amplificationMutant displaysP granulesNuclear peripheryRNA helicasesMissense mutantsMutant proteinsDouble mutantMUT-16Target mRNAsRNAiGerm cellsGenesEarly stepsGermline genesSiRNAsMutantsWidespread loss
2009
The Argonaute CSR-1 and Its 22G-RNA Cofactors Are Required for Holocentric Chromosome Segregation
Claycomb J, Batista P, Pang K, Gu W, Vasale J, van Wolfswinkel J, Chaves D, Shirayama M, Mitani S, Ketting R, Conte D, Mello C. The Argonaute CSR-1 and Its 22G-RNA Cofactors Are Required for Holocentric Chromosome Segregation. Cell 2009, 139: 123-134. PMID: 19804758, PMCID: PMC2766185, DOI: 10.1016/j.cell.2009.09.014.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsChromosome SegregationDEAD-box RNA HelicasesRNA-Dependent RNA PolymeraseConceptsArgonaute CSR-1Chromosome segregationHolocentric chromosomesCSR-1Protein-coding genesProper chromosome segregationProtein-coding domainsDRH-3EGO-1C. elegansSmall RNAsTarget lociTarget mRNAsSpindle polesDevelopmental timingMetaphase plateDiverse processesChromosomesKinetochoresProtein levelsProper organizationElegansChromatinDicerGenome