2014
MUT-14 and SMUT-1 DEAD Box RNA Helicases Have Overlapping Roles in Germline RNAi and Endogenous siRNA Formation
Phillips C, Montgomery B, Breen P, Roovers E, Rim Y, Ohsumi T, Newman M, van Wolfswinkel J, Ketting R, Ruvkun G, Montgomery T. MUT-14 and SMUT-1 DEAD Box RNA Helicases Have Overlapping Roles in Germline RNAi and Endogenous siRNA Formation. Current Biology 2014, 24: 839-844. PMID: 24684932, PMCID: PMC4010136, DOI: 10.1016/j.cub.2014.02.060.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCaenorhabditis elegansCaenorhabditis elegans ProteinsDEAD-box RNA HelicasesFluoroimmunoassayGerm CellsImmunoprecipitationMolecular Sequence DataReal-Time Polymerase Chain ReactionRNA InterferenceRNA, Small InterferingSaccharomyces cerevisiaeSequence AlignmentSequence Analysis, DNAConceptsSiRNA formationMutator fociClass genesDEAD-box RNA helicasesBox RNA helicasesElegans genesEndogenous siRNAsSiRNA amplificationMutant displaysP granulesNuclear peripheryRNA helicasesMissense mutantsMutant proteinsDouble mutantMUT-16Target mRNAsRNAiGerm cellsGenesEarly stepsGermline genesSiRNAsMutantsWidespread loss
2012
Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans
Kamminga LM, van Wolfswinkel JC, Luteijn MJ, Kaaij LJ, Bagijn MP, Sapetschnig A, Miska EA, Berezikov E, Ketting RF. Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans. PLOS Genetics 2012, 8: e1002702. PMID: 22829772, PMCID: PMC3400576, DOI: 10.1371/journal.pgen.1002702.Peer-Reviewed Original ResearchConceptsHENN-1G RNAAGO proteinsSRNA moleculesSmall RNAsGene expressionC. elegans piRNAsGermline-expressed genesSequence-specific recruitmentDifferent RNAi pathwaysGlobal gene expression analysisGlobal gene expressionLoss of methylationGene expression analysisEndogenous RNAiRNAi pathwayC. elegansRNA classesRNA displayGene activityRNA interferenceExpression analysisSequence specificityTarget RNARNA
2009
CDE-1 Affects Chromosome Segregation through Uridylation of CSR-1-Bound siRNAs
van Wolfswinkel JC, Claycomb JM, Batista PJ, Mello CC, Berezikov E, Ketting RF. CDE-1 Affects Chromosome Segregation through Uridylation of CSR-1-Bound siRNAs. Cell 2009, 139: 135-148. PMID: 19804759, DOI: 10.1016/j.cell.2009.09.012.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsCell Cycle ProteinsChromosome SegregationMeiosisMetaphaseMitosisRNA InterferenceRNA, Small InterferingRNA-Dependent RNA PolymeraseUridineConceptsCDE-1Chromosome segregationCSR-1RNAi pathwaySimilar sorting mechanismsMitotic chromosome segregationEndogenous RNAi pathwaysEGO-1Argonaute proteinsMitotic chromosomesSiRNA levelsC. elegansConserved natureSorting mechanismSiRNAsUridylationChromosomesInappropriate loadingProteinPathwaySegregationElegansRNAiMammalsGenesThe Argonaute CSR-1 and Its 22G-RNA Cofactors Are Required for Holocentric Chromosome Segregation
Claycomb J, Batista P, Pang K, Gu W, Vasale J, van Wolfswinkel J, Chaves D, Shirayama M, Mitani S, Ketting R, Conte D, Mello C. The Argonaute CSR-1 and Its 22G-RNA Cofactors Are Required for Holocentric Chromosome Segregation. Cell 2009, 139: 123-134. PMID: 19804758, PMCID: PMC2766185, DOI: 10.1016/j.cell.2009.09.014.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsChromosome SegregationDEAD-box RNA HelicasesRNA-Dependent RNA PolymeraseConceptsArgonaute CSR-1Chromosome segregationHolocentric chromosomesCSR-1Protein-coding genesProper chromosome segregationProtein-coding domainsDRH-3EGO-1C. elegansSmall RNAsTarget lociTarget mRNAsSpindle polesDevelopmental timingMetaphase plateDiverse processesChromosomesKinetochoresProtein levelsProper organizationElegansChromatinDicerGenome