2024
A circadian clock output functions independently of phyB to sustain daytime PIF3 degradation.
Liu W, Lowrey H, Xu A, Leung C, Adamchek C, He J, Du J, Chen M, Gendron J. A circadian clock output functions independently of phyB to sustain daytime PIF3 degradation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2024, 121: e2408322121. PMID: 39163340, PMCID: PMC11363348, DOI: 10.1073/pnas.2408322121.Peer-Reviewed Original ResearchConceptsPhytochrome-interacting factorsF-boxRed-light photoreceptor phytochrome BPhotoreceptor phytochrome BCircadian clock outputE3 ubiquitin ligaseDownstream biological processesPhyB signalingGrowth-regulating mechanismsTranscriptional connectionsPhytochrome BClock's roleRhythmic transcriptionUbiquitin ligaseClock regulationGenetic evidenceMonochromatic red lightProtein stabilityCircadian clockPhyBBiological processesPlant growthClock outputCircadian expressionRegulation mechanism
2020
A Decoy Library Uncovers U-Box E3 Ubiquitin Ligases That Regulate Flowering Time in Arabidopsis
Feke AM, Hong J, Liu W, Gendron JM. A Decoy Library Uncovers U-Box E3 Ubiquitin Ligases That Regulate Flowering Time in Arabidopsis. Genetics 2020, 215: 699-712. PMID: 32434795, PMCID: PMC7337086, DOI: 10.1534/genetics.120.303199.Peer-Reviewed Original ResearchMeSH KeywordsArabidopsisArabidopsis ProteinsFlowersGene Expression Regulation, DevelopmentalGene Expression Regulation, PlantReverse GeneticsUbiquitin-Protein LigasesConceptsE3 ubiquitin ligasesUbiquitin ligasesU-box E3 ubiquitin ligasesReproductive developmentReverse genetic screenMOS4-associated complexDominant-negative strategyGenetic redundancyGenetic screenTransgenic populationsTargeted degradationLigasesUnidentified regulatorDevelopmental transitionsBiological processesGenetic characterizationU-boxBiochemical studiesWidespread importanceRegulatorArabidopsisOrganismsPlantsProteinComplexes
2019
GIGANTEA recruits the UBP12 and UBP13 deubiquitylases to regulate accumulation of the ZTL photoreceptor complex
Lee CM, Li MW, Feke A, Liu W, Saffer AM, Gendron JM. GIGANTEA recruits the UBP12 and UBP13 deubiquitylases to regulate accumulation of the ZTL photoreceptor complex. Nature Communications 2019, 10: 3750. PMID: 31434902, PMCID: PMC6704089, DOI: 10.1038/s41467-019-11769-7.Peer-Reviewed Original ResearchMeSH KeywordsArabidopsisArabidopsis ProteinsCircadian ClocksEndopeptidasesGene Expression Regulation, PlantMutationTranscription FactorsUbiquitinationUbiquitin-Protein LigasesConceptsDeubiquitylating enzymesCircadian clockTarget proteinsE3 ubiquitin ligase activityPlant circadian clockUbiquitin-specific protease 12Post-transcriptional mechanismsUbiquitin ligase activityPhotoreceptor complexZTL proteinProtein ubiquitylationInteracting partnerProtein complexesLigase activityZEITLUPEUBP12Gi protein levelsUBP13Enzyme typeLight conditionsGiganteaProtein levelsProteinEnzymeDay light conditionsDecoys provide a scalable platform for the identification of plant E3 ubiquitin ligases that regulate circadian function
Feke A, Liu W, Hong J, Li MW, Lee CM, Zhou EK, Gendron JM. Decoys provide a scalable platform for the identification of plant E3 ubiquitin ligases that regulate circadian function. ELife 2019, 8: e44558. PMID: 30950791, PMCID: PMC6483598, DOI: 10.7554/elife.44558.Peer-Reviewed Original ResearchConceptsE3 ubiquitin ligasesUbiquitin ligasesCircadian clockCircadian functionPlant E3 ubiquitin ligasesTransgenic Arabidopsis plantsNew potential regulatorsArabidopsis plantsRegulated degradationPlant developmentClock proteinsClock regulatorsFunctional redundancyE3 ubiquitinProtein degradationGenetic challengesLigasesPotential regulatorCircadian periodScreening platformUbiquitinRegulatorDecoysSplicingClock