2021
Functional domain studies uncover novel roles for the ZTL Kelch repeat domain in clock function
Feke A, Vanderwall M, Liu W, Gendron JM. Functional domain studies uncover novel roles for the ZTL Kelch repeat domain in clock function. PLOS ONE 2021, 16: e0235938. PMID: 33730063, PMCID: PMC7968664, DOI: 10.1371/journal.pone.0235938.Peer-Reviewed Original ResearchConceptsLOV KELCH PROTEIN 2Kelch repeat domainRepeat domainLOV domainsF-BOX PROTEIN 1Protein-protein interaction domainsPlant circadian clockFLAVIN-BINDING KELCHProtein domain architecturePotential interacting partnerE3 ubiquitin ligasesInteracting partnerDomain architectureProtein complexesUbiquitin ligasesKelch proteinProtein familyTransgenic linesFlowering timeKelch familyProtein domainsZEITLUPEInteraction domainClock functionUnique photoreceptor
2019
GIGANTEA recruits the UBP12 and UBP13 deubiquitylases to regulate accumulation of the ZTL photoreceptor complex
Lee CM, Li MW, Feke A, Liu W, Saffer AM, Gendron JM. GIGANTEA recruits the UBP12 and UBP13 deubiquitylases to regulate accumulation of the ZTL photoreceptor complex. Nature Communications 2019, 10: 3750. PMID: 31434902, PMCID: PMC6704089, DOI: 10.1038/s41467-019-11769-7.Peer-Reviewed Original ResearchConceptsDeubiquitylating enzymesCircadian clockTarget proteinsE3 ubiquitin ligase activityPlant circadian clockUbiquitin-specific protease 12Post-transcriptional mechanismsUbiquitin ligase activityPhotoreceptor complexZTL proteinProtein ubiquitylationInteracting partnerProtein complexesLigase activityZEITLUPEUBP12Gi protein levelsUBP13Enzyme typeLight conditionsGiganteaProtein levelsProteinEnzymeDay light conditions
2017
Mapping Protein–Protein Interactions Using Affinity Purification and Mass Spectrometry
Lee CM, Adamchek C, Feke A, Nusinow DA, Gendron JM. Mapping Protein–Protein Interactions Using Affinity Purification and Mass Spectrometry. Methods In Molecular Biology 2017, 1610: 231-249. PMID: 28439867, DOI: 10.1007/978-1-4939-7003-2_15.Peer-Reviewed Original ResearchConceptsAP-MSHigher-order protein complexesAffinity purificationMapping protein-protein interactionsProtein-protein interaction networkProtein-protein interactionsMeaningful complexesProtein interactomeProtein complexesProtein-specific antibodiesMass spectrometry technologyInteraction networksCellular pathwaysTransient interactionsMass spectrometryInteractomeNonspecific interactionsSpectrometry technologyUnbiased methodComplexesPurificationComprehensive knowledgeCrucial stepPlantsProtein