2009
Combining electron crystallography and X-ray crystallography to study the MlotiK1 cyclic nucleotide-regulated potassium channel
Clayton GM, Aller SG, Wang J, Unger V, Morais-Cabral JH. Combining electron crystallography and X-ray crystallography to study the MlotiK1 cyclic nucleotide-regulated potassium channel. Journal Of Structural Biology 2009, 167: 220-226. PMID: 19545635, PMCID: PMC2922748, DOI: 10.1016/j.jsb.2009.06.012.Peer-Reviewed Original ResearchConceptsS1-S4 domainMembrane protein structure determinationTetrameric cation channelsX-ray crystallographyProtein structure determinationPotassium channelsElectron crystallographyMlotiK1Cation channelsX-ray structureNew insightsProjection mapStructure determinationCrystallographyCombined applicationDomainUseful strategyQuality crystals
1998
Crystal Structure Determination ofEscherichia coliClpP Starting from an EM-Derived Mask
Wang J, Hartling J, Flanagan J. Crystal Structure Determination ofEscherichia coliClpP Starting from an EM-Derived Mask. Journal Of Structural Biology 1998, 124: 151-163. PMID: 10049803, DOI: 10.1006/jsbi.1998.4058.Peer-Reviewed Original ResearchConceptsATP-dependent proteolytic complexEscherichia coli ClpPATP-dependent proteaseProteolytic active sitesEvolutionary convergenceClpP structureHeptameric ringsProteolytic complexIntracellular proteolysisProteolytic componentBiophysical techniquesClpPSmall-angle X-rayX-ray crystallographyX-ray crystal structureStriking exampleMatrix refinementActive siteProteaseStructure determinationHslVOverall architectureProteasomeStructural levelElectron microscopy