2017
Mechanisms of bile acid mediated inflammation in the liver
Li M, Cai SY, Boyer JL. Mechanisms of bile acid mediated inflammation in the liver. Molecular Aspects Of Medicine 2017, 56: 45-53. PMID: 28606651, PMCID: PMC5662014, DOI: 10.1016/j.mam.2017.06.001.Peer-Reviewed Original ResearchConceptsLiver injuryBile acidsCholestatic animal modelsCauses of cholestasisCholestatic liver injuryInnate immune cellsBiliary injuryNeutrophil recruitmentBile flowImmune cellsEffective therapyInflammatory responseAnimal modelsMolecular mediatorsCholestasisInjuryNovel targetLiverElevated levelsPathological processesMolecular mechanismsHepatocytesInflammationPatientsPathogenesis
2006
The organic anion transport polypeptide 1d1 (Oatp1d1) mediates hepatocellular uptake of phalloidin and microcystin into skate liver
Meier-Abt F, Hammann-Hänni A, Stieger B, Ballatori N, Boyer J. The organic anion transport polypeptide 1d1 (Oatp1d1) mediates hepatocellular uptake of phalloidin and microcystin into skate liver. Toxicology And Applied Pharmacology 2006, 218: 274-279. PMID: 17198718, DOI: 10.1016/j.taap.2006.11.015.Peer-Reviewed Original ResearchConceptsSkate liverToxic liver injuryFunctional transport activityLiver-specific OATPsOrganic anion transporting polypeptidesLiver injuryOrganic anion transportersWater-injected oocytesXenobiotic toxinsHepatocellular uptakeBile saltsSkate hepatocytesOATP substratesHuman brainLiverPresent studyOatps/OATPsAnion transportersOATPDemethylphalloinHepatocytesTransport activityToxinCellular uptakeUptake
1997
ATP regulation of a swelling‐activated osmolyte channel in skate hepatocytes
Ballatori N, Boyer J. ATP regulation of a swelling‐activated osmolyte channel in skate hepatocytes. Journal Of Experimental Zoology 1997, 279: 471-475. PMID: 9392868, DOI: 10.1002/(sici)1097-010x(19971201)279:5<471::aid-jez9>3.0.co;2-l.Peer-Reviewed Original ResearchConceptsChannel blockersTaurine effluxRegulatory volume decreaseVolume-activated taurine effluxRelease of taurineSkate hepatocytesIntracellular ATPIon channel blockersChloride permeability ratioIntracellular ATP levelsControl levelsOsmolyte channelIntracellular organic osmolytesWhole cell preparationsCell preparationsATP levelsBlockersHypotonic swellingChannel openingHepatocytesVolume decreaseAdditional mechanismEfflux
1996
Tauroursodeoxycholic acid activates protein kinase C in isolated rat hepatocytes
Beuers U, Throckmorton D, Anderson, Isales C, Thasler W, Kullak-Ublick G, Sauter G, Koebe H, Paumgartner G, Boyer J. Tauroursodeoxycholic acid activates protein kinase C in isolated rat hepatocytes. Gastroenterology 1996, 110: 1553-1563. PMID: 8613063, DOI: 10.1053/gast.1996.v110.pm8613063.Peer-Reviewed Original ResearchConceptsEffect of TUDCATauroursodeoxycholic acidProtein kinase CHepatocellular Ca2Chronic cholestatic liver diseaseCholestatic liver diseaseAlpha-PKCPKC activityLiver diseaseLiver functionRadioenzymatic techniqueExperimental cholestasisPhorbol ester phorbolActivation of PKCKinase CTaurocholic acidImmunofluorescence techniqueAbstractTextMembrane-associated PKC activitySignificant increaseAIMSStimulation of exocytosisUnknown mechanismParticulate membrane fractionHepatocytes
1994
Effects of protein kinase C and cytosolic Ca2+ on exocytosis in the isolated perfused rat liver
Bruck R, Nathanson M, Roelofsen H, Boyer J. Effects of protein kinase C and cytosolic Ca2+ on exocytosis in the isolated perfused rat liver. Hepatology 1994, 20: 1032-1040. PMID: 7927205, DOI: 10.1002/hep.1840200436.Peer-Reviewed Original ResearchConceptsProtein kinase CKinase CApical exocytosisRegulation of exocytosisProtein kinase C inhibitor HHorseradish peroxidase concentrationCytosolic Ca2ExocytosisCell typesInhibitor HProtein kinase C activator phorbol dibutyrateHorseradish peroxidase excretionPhorbol dibutyrateCa2Rat liverRelative importanceNet excretionPeroxidase concentrationsHepatocytesA volume-activated taurine channel in skate hepatocytes: membrane polarity and role of intracellular ATP
Ballatori N, Simmons T, Boyer J. A volume-activated taurine channel in skate hepatocytes: membrane polarity and role of intracellular ATP. American Journal Of Physiology 1994, 267: g285-g291. PMID: 7521136, DOI: 10.1152/ajpgi.1994.267.2.g285.Peer-Reviewed Original ResearchConceptsCell swellingSkate hepatocytesIntracellular ATPExtracellular taurine concentrationMetabolic inhibitorsSinusoidal circulationHypotonic stimuliTaurine concentrationsTaurine effluxTaurine uptakeIndependent uptakeTaurine channelSkate liverEfflux mechanismTaurineLiverHepatocytesInhibitorsEffluxBasolateral membraneOsmolyte taurineSwellingAntimycin ADisulfonic acidTransport inhibitorsCharacteristics of bile salt uptake into skate hepatocytes
Fricker G, Dubost V, Finsterwald K, Boyer J. Characteristics of bile salt uptake into skate hepatocytes. Biochemical Journal 1994, 299: 665-670. PMID: 8192655, PMCID: PMC1138072, DOI: 10.1042/bj2990665.Peer-Reviewed Original ResearchConceptsOrganic anion transport systemBile salt uptakeBile alcoholsBile saltsBeta-cholestane-3 alphaBile salt transportUnconjugated bile saltsTaurine-conjugated bile saltsHepatic uptakeAlpha-triolSkate bileInhibitory effectLiver cellsSkate hepatocytesIndependent transport systemsCholyltaurine transportCholyltaurineHepatocytesInhibitionAlphaUptakeSpecificityRegulation of activity and apical targeting of the Cl-/HCO3- exchanger in rat hepatocytes.
Benedetti A, Strazzabosco M, Ng O, Boyer J. Regulation of activity and apical targeting of the Cl-/HCO3- exchanger in rat hepatocytes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 792-796. PMID: 8290601, PMCID: PMC43035, DOI: 10.1073/pnas.91.2.792.Peer-Reviewed Original ResearchConceptsApical targetingExchange activityRegulation of activityMembrane localizationCl-/HCO3Dibutyryl cAMPProtein kinase C agonistsRat hepatocytesProtein kinase C agonist phorbolEcto-ATPaseConfocal microscopyCanalicular domainC agonistsProteinVesiclesTargetingCanalicular localizationLocalizationCAMPHepatocyte coupletsHepatocytesActivityRegulationPhorbolColchicine
1993
Effects of tauroursodeoxycholic acid on cytosolic Ca2+ signals in isolated rat hepatocytes
Beuers U, Nathanson M, Boyer J. Effects of tauroursodeoxycholic acid on cytosolic Ca2+ signals in isolated rat hepatocytes. Gastroenterology 1993, 104: 604-612. PMID: 8425704, DOI: 10.1016/0016-5085(93)90433-d.Peer-Reviewed Original ResearchConceptsEffect of TUDCATauroursodeoxycholic acidAdministration of TUDCAIP3-independent mechanismCytosolic free calciumIP3-sensitive Ca2Trihydroxy bile acidsCholestatic disordersHepatocyte secretionTreatment periodExtracellular Ca2Bile acidsTaurolithocholic acidMumol/Rat hepatocytesFree calciumBasal levelsTransient increaseCytosolic Ca2Levels of inositolFree mediumPhysiological concentrationsIsolated rat hepatocytesHepatocytesFourfold increase
1992
Taurine transport in skate hepatocytes. I. Uptake and efflux
Ballatori N, Boyer J. Taurine transport in skate hepatocytes. I. Uptake and efflux. American Journal Of Physiology 1992, 262: g445-g450. PMID: 1550234, DOI: 10.1152/ajpgi.1992.262.3.g445.Peer-Reviewed Original ResearchConceptsSkate hepatocytesDependent uptake processIntracellular taurine levelsTaurine uptake systemsTaurine levelsMicroM taurineTaurine uptakeIsosmotic replacementTaurine transportEfflux pathwaySlow effluxExtracellular ClIndependent uptake systemAmino sulfonic acidI. UptakeTaurine accumulationHepatocytesUptake systemEffluxTaurineDisulfonic acidBeta-amino acidsDistinct mechanismsUptakeReplacementEffects of Ca2+ agonists on cytosolic Ca2+ in isolated hepatocytes and on bile secretion in the isolated perfused rat liver
Nathanson M, Gautam A, Bruck R, Isales C, Boyer J. Effects of Ca2+ agonists on cytosolic Ca2+ in isolated hepatocytes and on bile secretion in the isolated perfused rat liver. Hepatology 1992, 15: 107-116. PMID: 1727785, DOI: 10.1002/hep.1840150119.Peer-Reviewed Original ResearchConceptsBile flowPerfusion pressureBile secretionCytosolic Ca2Rat liverProtein kinase CIonophore-induced increaseBromo-A23187Hepatic vasoconstrictionVasodilator papaverineProtein kinase C inhibitor HExtracellular Ca2Kinase CSecretionLiverVasopressinAgonist vasopressinBenzohydroquinoneEffect of Ca2HepatocytesActivate protein kinase CInhibitor HTwo-thirdsIsolated hepatocytesSensitive photoprotein aequorin
1991
Cl(-)-HCO3- exchanger in isolated rat hepatocytes: role in regulation of intracellular pH
Benedetti A, Strazzabosco M, Corasanti J, Haddad P, Graf J, Boyer J. Cl(-)-HCO3- exchanger in isolated rat hepatocytes: role in regulation of intracellular pH. American Journal Of Physiology 1991, 261: g512-g522. PMID: 1887897, DOI: 10.1152/ajpgi.1991.261.3.g512.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAmmoniaAnimalsBariumBicarbonatesCarrier ProteinsCells, CulturedChloride-Bicarbonate AntiportersChloridesHydrogen-Ion ConcentrationIntracellular FluidKineticsLiverMaleRatsRats, Inbred StrainsConceptsAlkaline loadPH-sensitive dye BCECFMM H2DIDSPHi recoveryRegulation of intracellularRat hepatocyte monolayersAcid extrudersMM DIDSRat hepatocytesElectrophysiological techniquesDye BCECFHCO3- exchangerIsolated rat hepatocytesConstant pHoIntracellularMembrane potentialHepatocyte monolayersHepatocytesAcid loadingEffect of UDCA on intracellular and biliary pH in isolated rat hepatocyte couplets and perfused livers
Strazzabosco M, Sakisaka S, Hayakawa T, Boyer J. Effect of UDCA on intracellular and biliary pH in isolated rat hepatocyte couplets and perfused livers. American Journal Of Physiology 1991, 260: g58-g69. PMID: 1987808, DOI: 10.1152/ajpgi.1991.260.1.g58.Peer-Reviewed Original ResearchConceptsBiliary HCO3- concentrationUrsodeoxycholic acidRat hepatocyte coupletsBiliary pHAcid loadEffect of UDCABile duct lumenHepatocyte coupletsIsolated rat hepatocyte coupletsBiliary epitheliumHCO3- concentrationDuct lumenRemoval of ClHCO3- exchangeRat liverLiverIPRLHepatocytesHypercholeresisPerfusateBileExcretionEpitheliumAmiloride
1990
Effects of osmotic stresses on isolated rat hepatocytes. I. Ionic mechanisms of cell volume regulation
Corasanti J, Gleeson D, Boyer J. Effects of osmotic stresses on isolated rat hepatocytes. I. Ionic mechanisms of cell volume regulation. American Journal Of Physiology 1990, 258: g290-g298. PMID: 2305895, DOI: 10.1152/ajpgi.1990.258.2.g290.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAmilorideAnimalsBariumBicarbonatesBumetanideCells, CulturedChloridesHypertonic SolutionsHypotonic SolutionsKineticsLiverMaleOsmolar ConcentrationPotassium ChlorideQuinineRatsRats, Inbred StrainsSodium ChlorideEffects of osmotic stresses on isolated rat hepatocytes. II. Modulation of intracellular pH
Gleeson D, Corasanti J, Boyer J. Effects of osmotic stresses on isolated rat hepatocytes. II. Modulation of intracellular pH. American Journal Of Physiology 1990, 258: g299-g307. PMID: 2305896, DOI: 10.1152/ajpgi.1990.258.2.g299.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsBicarbonatesBuffersCells, CulturedCholineHydrogen-Ion ConcentrationHypertonic SolutionsHypotonic SolutionsIntracellular FluidKineticsLiverMaleOsmolar ConcentrationRatsRats, Inbred StrainsSodiumConceptsRegulatory volume decreaseAbsence of HCO3Presence of HCO3Acid-base transport systemsModulation of intracellularRat hepatocytesVolume regulationAcute withdrawalMM amilorideGreater intracellularHCO3- exchangeAcid effluxIsotonic mediumIsolated rat hepatocytesSame maneuverCell volume regulationHepatocytesNet acid effluxAmilorideSubconfluent monolayersDisulfonic acidIntracellularVolume decreaseHypotonic mediumHypertonic medium
1989
Bicarbonate-dependent and -independent intracellular pH regulatory mechanisms in rat hepatocytes. Evidence for Na+-HCO3- cotransport.
Gleeson D, Smith N, Boyer J. Bicarbonate-dependent and -independent intracellular pH regulatory mechanisms in rat hepatocytes. Evidence for Na+-HCO3- cotransport. Journal Of Clinical Investigation 1989, 84: 312-321. PMID: 2544626, PMCID: PMC303984, DOI: 10.1172/jci114156.Peer-Reviewed Original ResearchConceptsDisulfonic acid stilbeneHCO3- cotransportIntracellular acid loadHCO3- thanIntracellular pH regulatory mechanismsPH regulatory mechanismsBaseline pHiMonolayer cell culture systemRat hepatocytesAcid loadIntracellular ClCl-/HCO3AmilorideCell culture systemAnion transport inhibitorAbsence of bicarbonateHCO3- transportImportant regulatorHepatocytesPH-sensitive dyeCarboxy-fluoresceinTransport inhibitorsCulture systemRegulatory mechanismsPhiInward-rectifying potassium channels in rat hepatocytes
Henderson R, Graf J, Boyer J. Inward-rectifying potassium channels in rat hepatocytes. American Journal Of Physiology 1989, 256: g1028-g1035. PMID: 2735409, DOI: 10.1152/ajpgi.1989.256.6.g1028.Peer-Reviewed Original ResearchConceptsRenal epithelial cellsRat hepatocytesWhole-cell conductanceWhole-cell experimentsSingle-channel recordingsEpithelial cellsPotassium channelsHigh intracellularFunctional heterogeneityOpen probabilityIsolated rat hepatocytesCellsWhole-cell modeInward rectificationMarked similarityStrong inward rectificationCell conductanceHepatocytesConductanceCalcium dependencePatch-clamp techniqueCurrent-voltage relationshipInward conductanceSpeciesCell experiments
1988
Effect of l-alanine on membrane potential, potassium (86Rb) permeability and cell volume in hepatocytes from Raja erinacea
Ballatori N, Wondergem R, Boyer J. Effect of l-alanine on membrane potential, potassium (86Rb) permeability and cell volume in hepatocytes from Raja erinacea. Biochimica Et Biophysica Acta 1988, 946: 261-269. PMID: 3207743, DOI: 10.1016/0005-2736(88)90401-4.Peer-Reviewed Original ResearchCharacteristics of L-alanine uptake in freshly isolated hepatocytes of elasmobranch Raja erinacea
Ballatori N, Boyer J. Characteristics of L-alanine uptake in freshly isolated hepatocytes of elasmobranch Raja erinacea. American Journal Of Physiology 1988, 254: r801-r808. PMID: 3364608, DOI: 10.1152/ajpregu.1988.254.5.r801.Peer-Reviewed Original ResearchConceptsNeutral amino acid transport systemElasmobranch Raja erinaceaMajor gluconeogenic precursorMM alanineSodium-dependent systemAmino acid transport systemsRaja erinaceaHepatic uptakeHepatic concentrationsAcid transport systemL-alanine uptakeAminotransferase activityTumor cellsSpecific transport mechanismsMeAIB transportGluconeogenic precursorsSaturable mechanismSkate hepatocytesHepatocytesAlanine metabolismGlucose releaseMeAIBHepatocyte suspensionsRat hepatocytesUptake
1987
Identification of a single sinusoidal bile salt uptake system in skate liver
Fricker G, Hugentobler G, Meier P, Kurz G, Boyer J. Identification of a single sinusoidal bile salt uptake system in skate liver. American Journal Of Physiology 1987, 253: g816-g822. PMID: 3425718, DOI: 10.1152/ajpgi.1987.253.6.g816.Peer-Reviewed Original ResearchConceptsBile salt uptakeSkate liverAlpha-dihydroxy-5 betaBile salt derivativesAzo-3 alphaBile acid uptakeConcentration-dependent mannerPredominant bile saltHepatic uptakePhotolabile bile salt derivativeIndependent transport mechanismsAcid uptakeLiverApparent molecular weightBile saltsRat liverTaurocholate uptakeSkate hepatocytesAnion transport inhibitorUptake systemHepatocytesPhotoaffinity labelingTaurocholateDisulfonic acidIrreversible inhibition