2010
Thinned-skull cranial window technique for long-term imaging of the cortex in live mice
Yang G, Pan F, Parkhurst CN, Grutzendler J, Gan WB. Thinned-skull cranial window technique for long-term imaging of the cortex in live mice. Nature Protocols 2010, 5: 201-208. PMID: 20134419, PMCID: PMC4690457, DOI: 10.1038/nprot.2009.222.Peer-Reviewed Original ResearchConceptsTwo-photon laser scanning microscopyHigh optical resolutionThinned-skull cranial windowImportant experimental toolOptical resolutionLiving brainCranial window techniqueLive miceLong-term imagingCranial windowExperimental toolInvasive approachAvoids exposureFunctional changesCortical structuresLongitudinal imagingPathological conditionsCortexBrainLaser scanning microscopyScanning microscopyMiceImagingMicroscopyResolution
2007
Long-term two-photon transcranial imaging of synaptic structures in the living brain.
Grutzendler J, Gan WB. Long-term two-photon transcranial imaging of synaptic structures in the living brain. Cold Spring Harbor Protocols 2007, 2007: pdb.prot4766. PMID: 21357119, DOI: 10.1101/pdb.prot4766.Peer-Reviewed Original ResearchLiving brainNeurological disease modelsMouse brain areasIndividual dendritic spinesAxonal varicositiesFrontal cortexDendritic spinesBrain areasTherapeutic interventionsSynaptic structureFunctional changesNeuronal structuresTranscranial imagingTwo-photon microscopyDisease modelsBrainLiving mouseSensitive toolImaging approachMemory processesImagingSomatosensoryVaricositiesCortexMice
2001
Two modes of radial migration in early development of the cerebral cortex
Nadarajah B, Brunstrom J, Grutzendler J, Wong R, Pearlman A. Two modes of radial migration in early development of the cerebral cortex. Nature Neuroscience 2001, 4: 143-150. PMID: 11175874, DOI: 10.1038/83967.Peer-Reviewed Original ResearchConceptsCerebral cortexAcute cortical slicesCertain genetic mutationsFinal laminar positionSite of originMovement of neuronsCortical slicesCortical neuronsRadial migrationCortical developmentLaminar positionSomal translocationGenetic mutationsCell-type specificDifferential effectsEarly ageCortexNeuronsMarginal zoneLeading processCellsTranslocationEarly developmentTime-lapse imaging