Featured Publications
Imaging and optogenetic modulation of vascular mural cells in the live brain
Tong L, Hill RA, Damisah EC, Murray KN, Yuan P, Bordey A, Grutzendler J. Imaging and optogenetic modulation of vascular mural cells in the live brain. Nature Protocols 2020, 16: 472-496. PMID: 33299155, DOI: 10.1038/s41596-020-00425-w.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsRegional cerebral blood flowMural cellsBlood-brain barrier maintenanceCerebral ischemia mouse modelAge-related neurodegenerative diseasesCerebral blood flowSmooth muscle cell physiologyBrain blood vesselsIschemia mouse modelVascular mural cellsBrain microvesselsHigh-resolution intravital imagingVascular disordersMouse modelBlood flowMuscle cell physiologyTransgenic miceCalcium transientsAlzheimer's diseaseCalcium imagingCell subtypesBarrier maintenanceNeurodegenerative diseasesTwo-photon optogeneticsBlood vesselsEmbolus extravasation is an alternative mechanism for cerebral microvascular recanalization
Lam CK, Yoo T, Hiner B, Liu Z, Grutzendler J. Embolus extravasation is an alternative mechanism for cerebral microvascular recanalization. Nature 2010, 465: 478-482. PMID: 20505729, PMCID: PMC2879083, DOI: 10.1038/nature09001.Peer-Reviewed Original ResearchConceptsAge-related cognitive disordersBlood vesselsPossible therapeutic targetAged miceBlood flowTherapeutic targetCognitive disordersMice showBlood clotsEndothelial cellsUnderlying endotheliumMicroemboliExtravasationFibrinolysisVesselsRecanalizationEmboliPatientsStrokeEndotheliumMiceBloodBrainWeeksClots
2016
“Small Blood Vessels: Big Health Problems?”: Scientific Recommendations of the National Institutes of Health Workshop
Bosetti F, Galis Z, Bynoe M, Charette M, Cipolla M, del Zoppo G, Gould D, Hatsukami T, Jones T, Koenig J, Lutty G, Maric‐Bilkan C, Stevens T, Tolunay H, Koroshetz W, Participants T, Agalliu D, Antonetti D, Boehm M, Brooks C, Caron K, Chilian W, Daemen M, D'Amato R, Davis T, Ergul A, Faber J, Gomez A, Grayson P, Grumbach I, Grutzendler J, Gu C, Gutterman D, Hallenbeck J, Herman I, Humphrey J, Iadecola C, Inscho E, Kleinfeld D, Lo E, Lopez J, Macknik S, Malik A, Mayadas T, McGavern D, Meininger G, Miller V, Nedergaard M, Nelson M, Peirce‐Cottler S, Ramadan I, Rosenberg G, Schiffrin E, Searson P, Stachenfeld N, Stan R, Suarez Y, Ubogu E, Vexler Z, Weyand C, Zlokovic B. “Small Blood Vessels: Big Health Problems?”: Scientific Recommendations of the National Institutes of Health Workshop. Journal Of The American Heart Association 2016, 5: e004389. PMID: 27815267, PMCID: PMC5210346, DOI: 10.1161/jaha.116.004389.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2011
Transcranial Two-Photon Imaging of the Living Mouse Brain
Grutzendler J, Yang G, Pan F, Parkhurst CN, Gan WB. Transcranial Two-Photon Imaging of the Living Mouse Brain. Cold Spring Harbor Protocols 2011, 2011: pdb.prot065474. PMID: 21880826, PMCID: PMC4641516, DOI: 10.1101/pdb.prot065474.Peer-Reviewed Original ResearchConceptsMouse brainAxonal varicositiesSurgical proceduresDendritic spinesSide effectsBrain cellsTwo-photon imagingInvasive methodBlood vesselsSame animalsPathological conditionsTwo-photon microscopyIntact skullBrainTranscranialImagingMicrogliaSurgeryVaricositiesImaging methodNeuronsLong time intervalsSpine