2001
Characterization of apical membrane Cl-dependent Na/H exchange in crypt cells of rat distal colon
Rajendran V, Geibel J, Binder H. Characterization of apical membrane Cl-dependent Na/H exchange in crypt cells of rat distal colon. AJP Gastrointestinal And Liver Physiology 2001, 280: g400-g405. PMID: 11171622, DOI: 10.1152/ajpgi.2001.280.3.g400.Peer-Reviewed Original ResearchConceptsRat distal colonNa/H exchangeCl- channel blockerDistal colonChannel blockersCrypt cellsIntracellular pH recoveryExchange inhibitor amilorideColonic epithelial cellsApical membrane vesiclesCrypt glandsHigh doseHOE 694NA depletionAcid loadExchange inhibitorInhibitor amiloridePH recoveryEpithelial cellsUptake 1NHE isoformsAnion exchange inhibitorBlockersColonNa uptake
1995
Chloride-dependent Na-H Exchange A NOVEL MECHANISM OF SODIUM TRANSPORT IN COLONIC CRYPTS*
Rajendran V, Geibel J, Binder H. Chloride-dependent Na-H Exchange A NOVEL MECHANISM OF SODIUM TRANSPORT IN COLONIC CRYPTS*. Journal Of Biological Chemistry 1995, 270: 11051-11054. PMID: 7744735, DOI: 10.1074/jbc.270.19.11051.Peer-Reviewed Original ResearchConceptsNa-H exchangeColonic crypt cellsCrypt cellsAcid loadRat distal colonApical membrane vesiclesNovel transport processRate of intracellularDistal colonMM amilorideSodium transportApical membraneGradient stimulationColonic cryptsAmiloride inhibitionSodium movementNovel mechanismOutward gradientCompelling evidenceCellsSodiumUptakePrior studiesColonRecovery
1993
Cl-HCO3 and Cl-OH exchanges mediate Cl uptake in apical membrane vesicles of rat distal colon
Rajendran V, Binder H. Cl-HCO3 and Cl-OH exchanges mediate Cl uptake in apical membrane vesicles of rat distal colon. American Journal Of Physiology 1993, 264: g874-g879. PMID: 8498514, DOI: 10.1152/ajpgi.1993.264.5.g874.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAmilorideAnimalsBicarbonatesBiological TransportBumetanideCarrier ProteinsCell MembraneChloride-Bicarbonate AntiportersChloridesColonDose-Response Relationship, DrugHydrogen-Ion ConcentrationKineticsMembrane ProteinsOxalatesRatsRats, Sprague-Dawley
1991
Sodium uptake across basolateral membrane of rat distal colon. Evidence for Na-H exchange and Na-anion cotransport.
Rajendran V, Oesterlin M, Binder H. Sodium uptake across basolateral membrane of rat distal colon. Evidence for Na-H exchange and Na-anion cotransport. Journal Of Clinical Investigation 1991, 88: 1379-1385. PMID: 1655829, PMCID: PMC295609, DOI: 10.1172/jci115444.Peer-Reviewed Original Research
1990
Characterization of Na-H exchange in apical membrane vesicles of rat colon.
Rajendran V, Binder H. Characterization of Na-H exchange in apical membrane vesicles of rat colon. Journal Of Biological Chemistry 1990, 265: 8408-8414. PMID: 2160456, DOI: 10.1016/s0021-9258(19)38902-1.Peer-Reviewed Original Research
1989
Regulation of active sodium and potassium transport in the distal colon of the rat. Role of the aldosterone and glucocorticoid receptors.
Turnamian S, Binder H. Regulation of active sodium and potassium transport in the distal colon of the rat. Role of the aldosterone and glucocorticoid receptors. Journal Of Clinical Investigation 1989, 84: 1924-1929. PMID: 2592566, PMCID: PMC304073, DOI: 10.1172/jci114380.Peer-Reviewed Original ResearchConceptsElectrogenic sodium absorptionReceptor-specific agonistsSodium absorptionAldosterone receptorsRU 28362Continuous infusionDistal colonGlucocorticoid receptorAmiloride-sensitive sodium absorptionColonic electrolyte transportEffects of aldosteroneRat distal colonPotassium transportActive sodiumPotassium secretionAldosteroneSustained increasePotassium absorptionAgonistsReceptorsElectrolyte transportGlucocorticoidsUnidirectional NaInfusionColonAldosterone induction of electrogenic sodium transport in the apical membrane vesicles of rat distal colon*
Rajendran V, Kashgarian M, Binder H. Aldosterone induction of electrogenic sodium transport in the apical membrane vesicles of rat distal colon*. Journal Of Biological Chemistry 1989, 264: 18638-18644. PMID: 2553716, DOI: 10.1016/s0021-9258(18)51514-3.Peer-Reviewed Original ResearchConceptsDistal colonRat distal colonNa-H exchangeElectrogenic sodium transportSodium transportExperimental ratsMicroM amilorideAmiloride-sensitive electrogenic sodium transportNormal rat distal colonEffects of aldosteroneElectroneutral sodium absorptionSodium concentrationApical membrane vesiclesAnalogues of amilorideVoltage-clamp conditionsDietary sodiumNormal ratsAldosterone inductionSodium absorptionColonComparable inhibitionRatsAmilorideAldosteronePotent inhibitorShort-chain fatty acids stimulate active sodium and chloride absorption in vitro in the rat distal colon
Binder H, Mehta P. Short-chain fatty acids stimulate active sodium and chloride absorption in vitro in the rat distal colon. Gastroenterology 1989, 96: 989-996. PMID: 2925072, DOI: 10.1016/0016-5085(89)91614-4.Peer-Reviewed Original ResearchConceptsRat distal colonShort-chain fatty acidsCl absorptionDistal colonNa absorptionNet NaFatty acid stimulationNa-H exchangeHCO3- Ringer's solutionStimulation of NaColonic NaNet Cl- absorptionVoltage-clamp conditionsCarbonic anhydrase inhibitorsFatty acidsCoupling of NaMucosal amilorideChloride absorptionUnidirectional 22NaClamp conditionsButyrate stimulationStimulationColonActive sodiumAcid stimulationEffects of corticosteroid hormones on the electrophysiology of rat distal colon: implications for Na+ and K+ transport.
Binder H, McGlone F, Sandle G. Effects of corticosteroid hormones on the electrophysiology of rat distal colon: implications for Na+ and K+ transport. The Journal Of Physiology 1989, 410: 425-441. PMID: 2795485, PMCID: PMC1190488, DOI: 10.1113/jphysiol.1989.sp017542.Peer-Reviewed Original ResearchConceptsRU 28362Rat distal colonDistal colonCorticosteroid hormonesChannel blocker tetraethylammonium chlorideEffects of aldosteroneCase of aldosteroneTypes of receptorsChannel blocker amilorideMineralocorticoid receptorEpithelial responseAldosteroneControl animalsGlucocorticoid receptorPattern of responseConventional microelectrodesCell membrane changesTetraethylammonium chlorideCorticosteroidsReceptorsSpironolactoneColonDexamethasoneHormonePotency
1988
Electrolyte transport in distal colon of sodium-depleted rats: effect of sodium repletion
Turnamian S, Binder H. Electrolyte transport in distal colon of sodium-depleted rats: effect of sodium repletion. American Journal Of Physiology 1988, 255: g329-g338. PMID: 2844099, DOI: 10.1152/ajpgi.1988.255.3.g329.Peer-Reviewed Original ResearchConceptsElectrogenic sodium absorptionElectroneutral sodium chloride absorptionPlasma aldosterone levelsAldosterone levelsSodium chloride absorptionPotassium secretionSodium absorptionSodium repletionDistal colonChloride absorptionElevated plasma aldosterone levelsSodium depletion increasesDistal colonic mucosaSodium-depleted ratsElectroneutral sodium absorptionRat distal colonExcess aldosteroneVoltage-clamp conditionsDietary sodiumColonic mucosaNormal animalsUnidirectional 22NaATPase activityElectrolyte transportSecretionGlucocorticoids have a different action than aldosterone on target tissue
Halevy J, Boulpaep E, Budinger M, Binder H, Hayslett J. Glucocorticoids have a different action than aldosterone on target tissue. American Journal Of Physiology 1988, 254: f153-f158. PMID: 3337244, DOI: 10.1152/ajprenal.1988.254.1.f153.Peer-Reviewed Original ResearchConceptsRU 28362Same doseInhibitory actionDexamethasone-treated animalsBody wt-1Effects of glucocorticoidsEffect of dexamethasoneReceptor-independent actionsRat distal colonSodium channel blockersMarked inhibitory actionEquivalent short-circuit currentBasolateral potential differenceAldosterone receptorsEpithelial changesDistal colonChannel blockersAldosteroneSynthetic glucocorticoidDay 1WT-1Large intestineGlucocorticoidsEpithelial resistanceMarked inhibition
1987
Mechanism of electroneutral sodium chloride absorption in distal colon of the rat
Binder H, Foster E, Budinger M, Hayslett J. Mechanism of electroneutral sodium chloride absorption in distal colon of the rat. Gastroenterology 1987, 93: 449-455. PMID: 3609655, DOI: 10.1016/0016-5085(87)90905-x.Peer-Reviewed Original ResearchConceptsSodium chloride absorptionElectroneutral sodium chloride absorptionNet sodium absorptionNet chloride absorptionDistal colonChloride absorptionSodium absorptionRat distal colonSodium chloride cotransportVoltage-clamp conditionsMM furosemideMM amilorideMucosal bathing solutionChloride transportClamp conditionsColonBathing solutionRatsElectrogenic sodiumMechanism of sodiumAmilorideSodiumAbsence of potassiumCotransportMucosa
1986
Dietary potassium modulates active potassium absorption and secretion in rat distal colon
Foster E, Sandle G, Hayslett J, Binder H. Dietary potassium modulates active potassium absorption and secretion in rat distal colon. American Journal Of Physiology 1986, 251: g619-g626. PMID: 3022598, DOI: 10.1152/ajpgi.1986.251.5.g619.Peer-Reviewed Original ResearchConceptsNet potassium absorptionNet potassium secretionPotassium secretionDistal colonic mucosaPotassium-loaded ratsRat distal colonPotassium transportActive potassium absorptionDietary potassiumPotassium absorptionColonic mucosaDistal colonMucosal amilorideLarge intestinePotassium depletionBody storesActive potassium transportSecretionBathing solutionPotassium loadingGroupK fluxesMucosaColonControlElectrophysiology of the human colon: evidence of segmental heterogeneity.
Sandle G, Wills N, Alles W, Binder H. Electrophysiology of the human colon: evidence of segmental heterogeneity. Gut 1986, 27: 999. PMID: 3758827, PMCID: PMC1433802, DOI: 10.1136/gut.27.9.999.Peer-Reviewed Original ResearchSodium-proton exchange in colon brush-border membranes
Binder H, Stange G, Murer H, Stieger B, Hauri H. Sodium-proton exchange in colon brush-border membranes. American Journal Of Physiology 1986, 251: g382-g390. PMID: 3019154, DOI: 10.1152/ajpgi.1986.251.3.g382.Peer-Reviewed Original ResearchSodium and chloride transport in the large intestine of potassium-loaded rats
Budinger M, Foster E, Hayslett J, Binder H. Sodium and chloride transport in the large intestine of potassium-loaded rats. American Journal Of Physiology 1986, 251: g249-g252. PMID: 2426972, DOI: 10.1152/ajpgi.1986.251.2.g249.Peer-Reviewed Original ResearchConceptsPotassium secretionSodium absorptionRat distal colonic mucosaElectroneutral sodium chloride absorptionPotassium loadingNa-K-ATPase activityElectrogenic sodium absorptionDistal colonic mucosaActive potassium secretionPotassium-loaded ratsNet sodium absorptionSodium chloride absorptionActive sodium transportVoltage-clamp conditionsDietary potassiumColonic functionColonic mucosaNet chloride fluxNet sodium fluxJNanetNet sodiumLarge intestineChloride fluxSodium transportIdentical increaseIon transport in proximal colon of the rat. Sodium depletion stimulates neutral sodium chloride absorption.
Foster E, Budinger M, Hayslett J, Binder H. Ion transport in proximal colon of the rat. Sodium depletion stimulates neutral sodium chloride absorption. Journal Of Clinical Investigation 1986, 77: 228-235. PMID: 2418060, PMCID: PMC423331, DOI: 10.1172/jci112281.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAcetazolamideAmilorideAnimalsBiological Transport, ActiveColonHyperaldosteronismIntestinal AbsorptionIntestinal MucosaIon ChannelsMaleRatsRats, Inbred StrainsSodium ChlorideTheophyllineConceptsMu eq/hSodium-hydrogen exchangeProximal colonSodium depletionSodium absorptionDistal colonNeutral sodium chloride absorptionDistal segmentsAmiloride-sensitive sodium absorptionNet sodium absorptionSodium-depleted ratsRat distal colonSodium chloride absorptionNeutral sodium absorptionHydrogen ion secretionSecondary hyperaldosteronismParallel fallRat colonControl groupMM amilorideMucosal amilorideJNanetColonIon secretionChloride absorption
1985
The electrical basis for enhanced potassium secretion in rat distal colon during dietary potassium loading
Sandle G, Foster E, Lewis S, Binder H, Hayslett J. The electrical basis for enhanced potassium secretion in rat distal colon during dietary potassium loading. Pflügers Archiv - European Journal Of Physiology 1985, 403: 433-439. PMID: 2409517, DOI: 10.1007/bf00589258.Peer-Reviewed Original ResearchConceptsRat distal colonDistal colonPotassium secretionDietary potassium loadingPotassium channel blocker tetraethylammonium chlorideBasolateral membrane voltageChannel blocker tetraethylammonium chloridePotassium loadingSodium channel blocker amilorideActive potassium secretionElectrical basisEnhanced potassium secretionChannel blocker amilorideMembrane conductanceIntracellular potassium activityApical membrane resistanceMucosal additionPotassium-selective microelectrodesBasal conditionsControl tissuesTransepithelial voltageColonMembrane voltageSecretionTetraethylammonium chlorideEvidence that aldosterone influences transport in target tissues by dissimilar mechanisms
Hirsch D, Pace P, Binder H, Hayslett J. Evidence that aldosterone influences transport in target tissues by dissimilar mechanisms. American Journal Of Physiology 1985, 248: f507-f512. PMID: 3985157, DOI: 10.1152/ajprenal.1985.248.4.f507.Peer-Reviewed Original ResearchConceptsProximal colonDistal colonSodium absorptionEffects of hyperaldosteronismSodium-free dietTarget tissuesAction of aldosteroneNet sodium absorptionRat proximal colonTransmural potential differenceAldosterone influencesHormone-induced changesSecondary hyperaldosteronismPotassium secretionControl animalsNet sodiumColonExperimental animalsHyperaldosteronismAldosteroneSodium transportProximal portionElectrolyte transportMicroA xTissue
1984
Apical membrane properties and amiloride binding kinetics of the human descending colon
Wills N, Alles W, Sandle G, Binder H. Apical membrane properties and amiloride binding kinetics of the human descending colon. American Journal Of Physiology 1984, 247: g749-g757. PMID: 6095681, DOI: 10.1152/ajpgi.1984.247.6.g749.Peer-Reviewed Original Research