2016
Statistical modeling and removal of lipid membrane projections for cryo-EM structure determination of reconstituted membrane proteins
Jensen KH, Brandt SS, Shigematsu H, Sigworth FJ. Statistical modeling and removal of lipid membrane projections for cryo-EM structure determination of reconstituted membrane proteins. Journal Of Structural Biology 2016, 194: 49-60. PMID: 26835990, PMCID: PMC4866491, DOI: 10.1016/j.jsb.2016.01.012.Peer-Reviewed Original Research
2013
Quantifying the local resolution of cryo-EM density maps
Kucukelbir A, Sigworth FJ, Tagare HD. Quantifying the local resolution of cryo-EM density maps. Nature Methods 2013, 11: 63-65. PMID: 24213166, PMCID: PMC3903095, DOI: 10.1038/nmeth.2727.Peer-Reviewed Original Research
2010
Single-Particle Cryo-EM Structure of a Membrane Protein in a Membrane
Wang L, Sigworth F. Single-Particle Cryo-EM Structure of a Membrane Protein in a Membrane. Microscopy And Microanalysis 2010, 16: 1076-1077. DOI: 10.1017/s1431927610060514.Peer-Reviewed Original ResearchA rail-to-rail operational amplifier in silicon-on-sapphire with constant transconductance
Weerakoon P, Sigworth F, Kindlmann P, Culurciello E. A rail-to-rail operational amplifier in silicon-on-sapphire with constant transconductance. Analog Integrated Circuits And Signal Processing 2010, 65: 311-319. DOI: 10.1007/s10470-010-9484-6.Peer-Reviewed Original ResearchEntire common mode rangeConstant transconductanceRail operational amplifierInput-referred voltage noiseCommon-mode rangeElectrical measurement resultsInput-referred offsetHigh-performance amplifiersMW of powerGain-bandwidth productOpen-loop gainMm2 of areaRail inputSOI technologySapphire (SOS) technologyOutput swingBiomedical instrumentationOperational amplifierVoltage noiseMeasurement resultsAmplifierTransconductanceSiliconRailFabricationA Two-Channel Patch-Clamp System on a Chip
Weerakoon P, Culurciello E, Santos-Sacchi J, Yang Y, Sigworth F. A Two-Channel Patch-Clamp System on a Chip. Biophysical Journal 2010, 98: 603a. DOI: 10.1016/j.bpj.2009.12.3288.Peer-Reviewed Original Research
2009
Structure of the BK potassium channel in a lipid membrane from electron cryomicroscopy
Wang L, Sigworth FJ. Structure of the BK potassium channel in a lipid membrane from electron cryomicroscopy. Nature 2009, 461: 292-295. PMID: 19718020, PMCID: PMC2797367, DOI: 10.1038/nature08291.Peer-Reviewed Original ResearchConceptsVoltage sensor domainNative membrane environmentMembrane proteinsMembrane environmentChannel voltage-sensor domainElectron cryomicroscopyVoltage-activated potassium channelsSingle-particle electron cryomicroscopyIndividual protein particlesPotassium channelsBK potassium channelsLarge conductance calciumLipid membranesX-ray crystal structureBK channelsStructural studiesProtein particlesProteinCryomicroscopyCrystal structureFormation of crystalsDomainStructureCrystalsChannels
2008
Integrated patch-clamp biosensor for high-density screening of cell conductance
Weerakoon P, Sigworth F, Culurciello E. Integrated patch-clamp biosensor for high-density screening of cell conductance. Electronics Letters 2008, 44: 81. DOI: 10.1049/el:20083151.Peer-Reviewed Original Research
2007
Ca-aktivierte K-Kanäle mittlerer Leitfähigkeit (IK): Klonierung und funktionelle Charakterisierung unterschiedlicher Isoformen in der apikalen und basolateralen Membran von Kolonepithelzellen
Barmeyer C, Rahner C, Yang Y, Sigworth F, Binder H, Rajendran V. Ca-aktivierte K-Kanäle mittlerer Leitfähigkeit (IK): Klonierung und funktionelle Charakterisierung unterschiedlicher Isoformen in der apikalen und basolateralen Membran von Kolonepithelzellen. Zeitschrift Für Gastroenterologie 2007, 45 DOI: 10.1055/s-2007-988400.Peer-Reviewed Original Research
2006
Using cryo-EM to measure the dipole potential of a lipid membrane
Wang L, Bose PS, Sigworth FJ. Using cryo-EM to measure the dipole potential of a lipid membrane. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 18528-18533. PMID: 17116859, PMCID: PMC1693696, DOI: 10.1073/pnas.0608714103.Peer-Reviewed Original ResearchConceptsDipole potentialLipid membranesPhase-contrast imagingMolecular dynamics calculationsLipid bilayer membranesIon translocation ratesAtomic potentialLipid moleculesLipid monolayersBilayer membranesDynamics calculationsProbe methodSurface potentialAnionsDiphytanoylphosphatidylcholineCationsElectronsImage intensityAbsolute valueMembraneMoleculesConformationLarge permeabilityMonolayersBilayers
2002
Applying Hidden Markov Models to the Analysis of Single Ion Channel Activity
Venkataramanan L, Sigworth F. Applying Hidden Markov Models to the Analysis of Single Ion Channel Activity. Biophysical Journal 2002, 82: 1930-1942. PMID: 11916851, PMCID: PMC1301989, DOI: 10.1016/s0006-3495(02)75542-2.Peer-Reviewed Original ResearchConceptsCorrelated background noiseHidden Markov ModelDigital inverse filterMarkov model parametersDiscrete timeBaum-Welch algorithmMarkov modelComputational intensityDeterministic interferenceModel parametersInverse filterMultiple data setsAlgorithmPrevious resultsPractical applicationsNoise ratioState transitionsSharp frequencyData setsChannel dataSingle ion channel currentsNoiseExtensionRandomnessBackground noise
2000
Identification of hidden Markov models for ion channel currents .III. Bandlimited, sampled data
Venkataramanan L, Kuc R, Sigworth F. Identification of hidden Markov models for ion channel currents .III. Bandlimited, sampled data. IEEE Transactions On Signal Processing 2000, 48: 376-385. DOI: 10.1109/78.823965.Peer-Reviewed Original Research
1999
Two-Microelectrode Voltage Clamp of Xenopus Oocytes: Voltage Errors and Compensation for Local Current Flow
Baumgartner W, Islas L, Sigworth F. Two-Microelectrode Voltage Clamp of Xenopus Oocytes: Voltage Errors and Compensation for Local Current Flow. Biophysical Journal 1999, 77: 1980-1991. PMID: 10512818, PMCID: PMC1300479, DOI: 10.1016/s0006-3495(99)77039-6.Peer-Reviewed Original ResearchGramicidin channel controversy — the structure in a lipid environment
Andersen O, Apell H, Bamberg E, Busath D, Koeppe R, Sigworth F, Szabo G, Urry D, Woolley A. Gramicidin channel controversy — the structure in a lipid environment. Nature Structural & Molecular Biology 1999, 6: 609-609. PMID: 10404209, DOI: 10.1038/10648.Peer-Reviewed Original Research
1998
Identification of hidden Markov models for ion channel currents. I. Colored background noise
Venkataramanan L, Walsh J, Kuc R, Sigworth F. Identification of hidden Markov models for ion channel currents. I. Colored background noise. IEEE Transactions On Signal Processing 1998, 46: 1901-1915. DOI: 10.1109/78.700963.Peer-Reviewed Original ResearchBaum-Welch algorithmMarkov schemeMarkov processMarkov modelIon channel kineticsColored background noiseUnbiased estimatesHMM parametersBiased estimatesSignal processingHMM approachAlgorithmTraditional algorithmsNoiseNoise ratioIon channel currentsSingle ion channel currentsConventional analysisBackground noiseCurrentParametersEstimatesModelEstimationSchemeIdentification of hidden Markov models for ion channel currents. II. State-dependent excess noise
Venkataramanan L, Kuc R, Sigworth F. Identification of hidden Markov models for ion channel currents. II. State-dependent excess noise. IEEE Transactions On Signal Processing 1998, 46: 1916-1929. DOI: 10.1109/78.700964.Peer-Reviewed Original ResearchActivation of Shaker Potassium Channels
Schoppa N, Sigworth F. Activation of Shaker Potassium Channels. The Journal Of General Physiology 1998, 111: 295-311. PMID: 9450945, PMCID: PMC2222768, DOI: 10.1085/jgp.111.2.295.Peer-Reviewed Original ResearchActivation of Shaker Potassium Channels
Schoppa N, Sigworth F. Activation of Shaker Potassium Channels. The Journal Of General Physiology 1998, 111: 271-294. PMID: 9450944, PMCID: PMC2222764, DOI: 10.1085/jgp.111.2.271.Peer-Reviewed Original ResearchActivation of Shaker Potassium Channels
Schoppa N, Sigworth F. Activation of Shaker Potassium Channels. The Journal Of General Physiology 1998, 111: 313-342. PMID: 9450946, PMCID: PMC2222769, DOI: 10.1085/jgp.111.2.313.Peer-Reviewed Original ResearchA Maximum-Likelihood Approach to Single-Particle Image Refinement
Sigworth F. A Maximum-Likelihood Approach to Single-Particle Image Refinement. Journal Of Structural Biology 1998, 122: 328-339. PMID: 9774537, DOI: 10.1006/jsbi.1998.4014.Peer-Reviewed Original Research
1997
Enhanced Closed-state Inactivation in a Mutant Shaker K+ Channel
Ayer, Jr. R, Sigworth F. Enhanced Closed-state Inactivation in a Mutant Shaker K+ Channel. The Journal Of Membrane Biology 1997, 157: 215-230. PMID: 9178609, DOI: 10.1007/s002329900230.Peer-Reviewed Original Research