2023
A ticking time bomb hidden in plain sight
Narasimhan S, Fish D, Pedra J, Pal U, Fikrig E. A ticking time bomb hidden in plain sight. Science Translational Medicine 2023, 15: eadi7829. PMID: 37851823, DOI: 10.1126/scitranslmed.adi7829.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2014
Borrelia miyamotoi sensu lato Seroreactivity and Seroprevalence in the Northeastern United States - Volume 20, Number 7—July 2014 - Emerging Infectious Diseases journal - CDC
Krause PJ, Narasimhan S, Wormser GP, Barbour AG, Platonov AE, Brancato J, Lepore T, Dardick K, Mamula M, Rollend L, Steeves TK, Diuk-Wasser M, Usmani-Brown S, Williamson P, Sarksyan DS, Fikrig E, Fish D, . Borrelia miyamotoi sensu lato Seroreactivity and Seroprevalence in the Northeastern United States - Volume 20, Number 7—July 2014 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2014, 20: 1183-1190. PMID: 24960072, PMCID: PMC4073859, DOI: 10.3201/eid2007.131587.Peer-Reviewed Original ResearchConceptsB. burgdorferiInfectious Diseases journal - CDCSeroprevalence of IgGB. burgdorferi antigensLyme disease endemic areaDisease-endemic areasAntibody testingHealthy personsLyme diseaseSerum samplesSeroprevalenceBurgdorferiInfectionSame tickUnited StatesSensu latoArea residentsPersonsBorrelia spSeroreactivityIgGAntigenDiseaseSerumGut Microbiota of the Tick Vector Ixodes scapularis Modulate Colonization of the Lyme Disease Spirochete
Narasimhan S, Rajeevan N, Liu L, Zhao YO, Heisig J, Pan J, Eppler-Epstein R, DePonte K, Fish D, Fikrig E. Gut Microbiota of the Tick Vector Ixodes scapularis Modulate Colonization of the Lyme Disease Spirochete. Cell Host & Microbe 2014, 15: 58-71. PMID: 24439898, PMCID: PMC3905459, DOI: 10.1016/j.chom.2013.12.001.Peer-Reviewed Original ResearchConceptsPeritrophic matrixTranscription factor signal transducerPathogen colonizationLyme disease spirochete Borrelia burgdorferiActivator of transcriptionGut microbiotaArthropod gutsSignal transducerLyme disease spirocheteFunctional linkArthropod vectorsMajor vectorKey glycoproteinsHuman pathogensSpirochete Borrelia burgdorferiGut epitheliumIxodes scapularis ticksColonizationGut epithelial barrierMicrobiotaExpressionGut lumenScapularis ticksBorrelia burgdorferiEpithelial barrier
2013
Human Borrelia miyamotoi Infection in the United States
Krause PJ, Narasimhan S, Wormser GP, Rollend L, Fikrig E, Lepore T, Barbour A, Fish D. Human Borrelia miyamotoi Infection in the United States. New England Journal Of Medicine 2013, 368: 291-293. PMID: 23323920, PMCID: PMC3934646, DOI: 10.1056/nejmc1215469.Peer-Reviewed Original Research
2011
Anaplasma phagocytophilum induces actin phosphorylation to selectively regulate gene transcription in Ixodes scapularis ticks
Sultana H, Neelakanta G, Kantor F, Malawista S, Fish D, Montgomery R, Fikrig E. Anaplasma phagocytophilum induces actin phosphorylation to selectively regulate gene transcription in Ixodes scapularis ticks. Journal Of Experimental Medicine 2011, 208: 1737-1737. PMCID: PMC3149219, DOI: 10.1084/jem.201002762088c.Peer-Reviewed Original Research
2010
Anaplasma phagocytophilum induces Ixodes scapularis ticks to express an antifreeze glycoprotein gene that enhances their survival in the cold
Neelakanta G, Sultana H, Fish D, Anderson JF, Fikrig E. Anaplasma phagocytophilum induces Ixodes scapularis ticks to express an antifreeze glycoprotein gene that enhances their survival in the cold. Journal Of Clinical Investigation 2010, 120: 3179-3190. PMID: 20739755, PMCID: PMC2929727, DOI: 10.1172/jci42868.Peer-Reviewed Original ResearchAnaplasma phagocytophilum induces actin phosphorylation to selectively regulate gene transcription in Ixodes scapularis ticks
Sultana H, Neelakanta G, Kantor F, Malawista S, Fish D, Montgomery R, Fikrig E. Anaplasma phagocytophilum induces actin phosphorylation to selectively regulate gene transcription in Ixodes scapularis ticks. Journal Of Cell Biology 2010, 190: i8-i8. DOI: 10.1083/jcb1903oia8.Peer-Reviewed Original ResearchAnaplasma phagocytophilum induces actin phosphorylation to selectively regulate gene transcription in Ixodes scapularis ticks
Sultana H, Neelakanta G, Kantor FS, Malawista SE, Fish D, Montgomery RR, Fikrig E. Anaplasma phagocytophilum induces actin phosphorylation to selectively regulate gene transcription in Ixodes scapularis ticks. Journal Of Experimental Medicine 2010, 207: 1727-1743. PMID: 20660616, PMCID: PMC2916137, DOI: 10.1084/jem.20100276.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnaplasma phagocytophilumAnimalsCell LineCell NucleusEnzyme InhibitorsGastrointestinal TractGene ExpressionGene Expression RegulationGTP-Binding Protein beta SubunitsGTP-Binding Protein gamma SubunitsInsect ProteinsIxodesP21-Activated KinasesPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPhosphorylationPromoter Regions, GeneticProtein BindingRNA InterferenceRNA Polymerase IISalivary GlandsSalivary Proteins and PeptidesSignal TransductionTATA-Box Binding ProteinTranscription, GeneticConceptsRNA polymerase IIActin phosphorylationTATA box-binding proteinNuclear G-actinPhosphorylation of actinP21-activated kinaseA. phagocytophilumA. phagocytophilum survivalTick cell linesIxodes scapularis ticksPolymerase IIPhosphorylated actinGene crucialGbetagamma subunitsGene transcriptionFilamentous actinAnaplasma phagocytophilumGene expressionBacterial acquisitionScapularis ticksPhosphorylationG-actinIntracellular pathogensMedical importanceActin
2007
Role of Outer Surface Protein D in the Borrelia burgdorferi Life Cycle
Li X, Neelakanta G, Liu X, Beck DS, Kantor FS, Fish D, Anderson JF, Fikrig E. Role of Outer Surface Protein D in the Borrelia burgdorferi Life Cycle. Infection And Immunity 2007, 75: 4237-4244. PMID: 17620358, PMCID: PMC1951184, DOI: 10.1128/iai.00632-07.Peer-Reviewed Original ResearchConceptsB. burgdorferiSecond blood mealMurine modelSpirochete transmissionB. burgdorferi persistenceLyme borreliosisMiceB. burgdorferi strainsProtein DI. scapularisTick gutBlood mealBurgdorferi strainsSpirochete life cycleBurgdorferiGut extractsIxodes scapularisB. burgdorferi infectivityThreefold decreaseHost-specific signalsScapularisTicksBorreliosisOuter Surface Protein B Is Critical for Borrelia burgdorferi Adherence and Survival within Ixodes Ticks
Neelakanta G, Li X, Pal U, Liu X, Beck DS, DePonte K, Fish D, Kantor FS, Fikrig E. Outer Surface Protein B Is Critical for Borrelia burgdorferi Adherence and Survival within Ixodes Ticks. PLOS Pathogens 2007, 3: e33. PMID: 17352535, PMCID: PMC1817655, DOI: 10.1371/journal.ppat.0030033.Peer-Reviewed Original Research
2006
An Ixodes scapularis protein required for survival of Anaplasma phagocytophilum in tick salivary glands
Sukumaran B, Narasimhan S, Anderson JF, DePonte K, Marcantonio N, Krishnan MN, Fish D, Telford SR, Kantor FS, Fikrig E. An Ixodes scapularis protein required for survival of Anaplasma phagocytophilum in tick salivary glands. Journal Of Experimental Medicine 2006, 203: 1507-1517. PMID: 16717118, PMCID: PMC2118316, DOI: 10.1084/jem.20060208.Peer-Reviewed Original ResearchConceptsA. phagocytophilum-infected miceRNA interference-mediated silencingA. phagocytophilumTick salivary proteinsI. scapularis salivary glandsRickettsia-like pathogensTick salivary glandsMammalian hostsGenus RickettsiaAnaplasma phagocytophilumGene expressionSalivary glandsIntracellular organismsArthropodsSalivary proteinsPathogensProteinPhagocytophilumExpressionTicksHuman anaplasmosisSilencingGenesOrganismsAnaplasma
2005
The Lyme disease agent exploits a tick protein to infect the mammalian host
Ramamoorthi N, Narasimhan S, Pal U, Bao F, Yang XF, Fish D, Anguita J, Norgard MV, Kantor FS, Anderson JF, Koski RA, Fikrig E. The Lyme disease agent exploits a tick protein to infect the mammalian host. Nature 2005, 436: 573-577. PMID: 16049492, PMCID: PMC4306560, DOI: 10.1038/nature03812.Peer-Reviewed Original Research
2002
Examination of the Borrelia burgdorferi Transcriptome in Ixodes scapularis during Feeding
Narasimhan S, Santiago F, Koski RA, Brei B, Anderson JF, Fish D, Fikrig E. Examination of the Borrelia burgdorferi Transcriptome in Ixodes scapularis during Feeding. Journal Of Bacteriology 2002, 184: 3122-3125. PMID: 12003955, PMCID: PMC135063, DOI: 10.1128/jb.184.11.3122-3125.2002.Peer-Reviewed Original ResearchConceptsBorrelia burgdorferi gene expressionB. burgdorferi genesPeriplasmic proteinsSignal transductionChromosomal genesPutative lipoproteinSubstrate transportGene expressionDifferential expressionGenesEnergy metabolismIxodes scapularis ticksGlobal analysisIxodes scapularisScapularis ticksExpressionTranscriptomeTransductionTicksProteinFeedingMetabolismScapularis
2001
OspA Immunization Decreases Transmission of Borrelia burgdorferi Spirochetes from Infected Peromyscus leucopus Mice to Larval Ixodes scapularis Ticks
Tsao J, Barbour A, Luke C, Fikrig E, Fish D. OspA Immunization Decreases Transmission of Borrelia burgdorferi Spirochetes from Infected Peromyscus leucopus Mice to Larval Ixodes scapularis Ticks. Vector-Borne And Zoonotic Diseases 2001, 1: 65-74. PMID: 12653137, DOI: 10.1089/153036601750137705.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, WildAntibodies, BacterialAntigens, SurfaceArachnid VectorsBacterial Outer Membrane ProteinsBacterial VaccinesBorrelia burgdorferiDisease ReservoirsDose-Response Relationship, ImmunologicEnzyme-Linked Immunosorbent AssayFluorescent Antibody Technique, DirectHost-Parasite InteractionsIxodesLarvaLipoproteinsLyme DiseasePeromyscusConceptsControl miceUninfected larval ticksInfection prevalenceLarval Ixodes scapularis ticksB. burgdorferiNumber of spirochetesEnzyme-linked immunosorbentBorrelia burgdorferi spirochetesLevel of immunizationReservoir hostsConcentration of antibodyBorrelia burgdorferi transmissionIxodes scapularis ticksSecond vaccinationImmunized miceInfected miceLarval ticksPrior infectionVaccination programSubsequent riskDecrease transmissionOspA vaccinationDirect immunofluorescenceImmune responseXenodiagnostic ticks
2000
Attachment of Borrelia burgdorferi within Ixodes scapularis mediated by outer surface protein A
Pal U, de Silva A, Montgomery R, Fish D, Anguita J, Anderson J, Lobet Y, Fikrig E. Attachment of Borrelia burgdorferi within Ixodes scapularis mediated by outer surface protein A. Journal Of Clinical Investigation 2000, 106: 561-569. PMID: 10953031, PMCID: PMC380253, DOI: 10.1172/jci9427.Peer-Reviewed Original Research
1998
Prevention of Borrelia burgdorferi transmission in guinea pigs by tick immunity.
Nazario S, Das S, de Silva AM, Deponte K, Marcantonio N, Anderson JF, Fish D, Fikrig E, Kantor FS. Prevention of Borrelia burgdorferi transmission in guinea pigs by tick immunity. American Journal Of Tropical Medicine And Hygiene 1998, 58: 780-5. PMID: 9660463, DOI: 10.4269/ajtmh.1998.58.780.Peer-Reviewed Original ResearchGranulocytic Ehrlichiosis in the Laboratory Mouse
Hodzic E, IJdo J, Feng S, Katavolos P, Sun W, Maretzki C, Fish D, Fikrig E, Telford S, Barthold S. Granulocytic Ehrlichiosis in the Laboratory Mouse. The Journal Of Infectious Diseases 1998, 177: 737-745. PMID: 9498456, DOI: 10.1086/514236.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisMouse passageGranulocytic ehrlichiosisHL-60 cell culturesPeripheral bloodC3H miceLate infectionEarly infectionCell culturesDay 17Day 5Blood smearsNormal valuesDay 24Polymerase chain reaction amplificationHGE agentChain reaction amplificationSpleen smearsMiceInfectionInfectivity assaysBloodLaboratory miceSmearsReaction amplification
1997
OspA antibodies inhibit the acquisition of Borrelia burgdorferi by Ixodes ticks
de Silva AM, Fish D, Burkot TR, Zhang Y, Fikrig E. OspA antibodies inhibit the acquisition of Borrelia burgdorferi by Ixodes ticks. Infection And Immunity 1997, 65: 3146-3150. PMID: 9234767, PMCID: PMC175444, DOI: 10.1128/iai.65.8.3146-3150.1997.Peer-Reviewed Original ResearchConceptsOspA antibodiesB. burgdorferiPassive antibody transferB. burgdorferi infectionIxodes ticksSurface protein ABurgdorferi-infected miceB. burgdorferi-infected miceLarval infection ratesOuter surface protein AAntibody transferAntibody responseBurgdorferi infectionInfection rateInfected ticksMiceInhibitory effectAntibodiesLarval infectionBorrelia burgdorferiBurgdorferiInfectionLarval ticksReservoir competenceSpirochetes