2013
F1FO ATPase vesicle preparation and technique for performing patch clamp recordings of submitochondrial vesicle membranes.
Sacchetti S, Alavian KN, Lazrove E, Jonas EA. F1FO ATPase vesicle preparation and technique for performing patch clamp recordings of submitochondrial vesicle membranes. Journal Of Visualized Experiments 2013, e4394. PMID: 23685483, PMCID: PMC3676267, DOI: 10.3791/4394.Peer-Reviewed Original ResearchConceptsF1Fo-ATP synthaseATP synthaseF1Fo-ATPaseSubmitochondrial vesiclesNecrotic cell deathPro-apoptotic factorsCell deathOuter membraneBcl-2 family proteinsMitochondrial outer membraneImportant cellular functionsOuter membrane ruptureImportant mitochondrial functionsRole of mitochondriaMediation of signalsMitochondrial permeability transition poreProduction of ATPApoptotic cell deathPermeability transition poreInner membrane poreCellular functionsFamily proteinsInner membraneOxidative phosphorylationBeta subunit
2012
Multipolar functions of BCL-2 proteins link energetics to apoptosis
Hardwick JM, Chen YB, Jonas EA. Multipolar functions of BCL-2 proteins link energetics to apoptosis. Trends In Cell Biology 2012, 22: 318-328. PMID: 22560661, PMCID: PMC3499971, DOI: 10.1016/j.tcb.2012.03.005.Peer-Reviewed Original ResearchConceptsBcl-2 proteinClassical apoptotic cell deathBcl-2 family proteinsSub-mitochondrial localizationApoptotic cell deathFamily proteinsClassical apoptosisBiochemical activityApoptosis regulatorCritical crosstalkCell survivalCell deathProteinApoptosisProfound effectCellsMultipolar functionsRegulatorCrosstalkLocalizationCurrent assumptionsDeath
2009
Bcl-xL increases mitochondrial fission, fusion, and biomass in neurons
Berman SB, Chen YB, Qi B, McCaffery JM, Rucker EB, Goebbels S, Nave KA, Arnold BA, Jonas EA, Pineda FJ, Hardwick JM. Bcl-xL increases mitochondrial fission, fusion, and biomass in neurons. Journal Of Cell Biology 2009, 184: 707-719. PMID: 19255249, PMCID: PMC2686401, DOI: 10.1083/jcb.200809060.Peer-Reviewed Original ResearchConceptsMitochondrial fissionMitochondrial morphologyCell deathApoptotic cell deathRate of fissionMitochondrial organellesOrganelle morphologyMitochondrial biomassBcl-xLCell typesFluorescence microscopyHealthy neuronsBclCultured neuronsDependent mechanismNeuronal dysfunctionFissionNeuronal processesBiomassSynaptic activityFusionOrganellesComputational strategyRate of fusionRegulation