2024
Randomized Phase II Trial of Imiquimod with or without 9-Valent HPV Vaccine versus Observation in Patients with High-grade Pre-neoplastic Cervical Lesions (NCT02864147)
Sheth S, Oh J, Bellone S, Siegel E, Greenman M, Mutlu L, McNamara B, Pathy S, Clark M, Azodi M, Altwerger G, Andikyan V, Huang G, Ratner E, Kim D, Iwasaki A, Levi A, Buza N, Hui P, Flaherty S, Schwartz P, Santin A. Randomized Phase II Trial of Imiquimod with or without 9-Valent HPV Vaccine versus Observation in Patients with High-grade Pre-neoplastic Cervical Lesions (NCT02864147). Clinical Cancer Research 2024, 30: of1-of10. PMID: 38592381, DOI: 10.1158/1078-0432.ccr-23-3639.Peer-Reviewed Original ResearchConceptsRandomized phase II trialCD4/CD8 T cellsT cellsHPV clearanceArm BNo significant differenceClinical surveillanceRate of HPV clearanceSecondary outcomesPre-neoplastic cervical lesionsCervical intraepithelial neoplasiaT cell infiltrationT cell responsesSignificant differenceCIN3 patientsIntraepithelial neoplasiaArm ACervical lesionsImiquimod groupSurveillance armVaginal suppositoriesProspective trialsArm CHPV vaccinationImiquimod
2022
Prediction of Adverse Pathology at Radical Prostatectomy in Grade Group 2 and 3 Prostate Biopsies Using Machine Learning.
Paulson N, Zeevi T, Papademetris M, Leapman MS, Onofrey JA, Sprenkle PC, Humphrey PA, Staib LH, Levi AW. Prediction of Adverse Pathology at Radical Prostatectomy in Grade Group 2 and 3 Prostate Biopsies Using Machine Learning. JCO Clinical Cancer Informatics 2022, 6: e2200016. PMID: 36179281, DOI: 10.1200/cci.22.00016.Peer-Reviewed Original ResearchConceptsGrade group 2Prostate biopsyRadical prostatectomyAdverse outcomesGroup 2GG-2Core prostate biopsyProstate cancer outcomesPatient's clinical riskClinical risk assessmentCore needle biopsyOngoing clinical needAdverse outcome predictionRetrospective reviewAdverse pathologyCAPRA scoreEntire cohortCancer outcomesPathologic diagnosisNeedle biopsyClinical riskDisease outcomeProstate cancerBiopsyDisease one
2021
Extent of High-Grade Prostatic Adenocarcinoma in Multiparametric Magnetic Resonance Imaging-Targeted Biopsy Enhances Prediction of Pathologic Stage.
Paulson N, Vollmer RT, Humphrey PA, Sprenkle PC, Onofrey J, Huber S, Amirkhiz K, Levi AW. Extent of High-Grade Prostatic Adenocarcinoma in Multiparametric Magnetic Resonance Imaging-Targeted Biopsy Enhances Prediction of Pathologic Stage. Archives Of Pathology & Laboratory Medicine 2021, 146: 201-204. PMID: 34015819, DOI: 10.5858/arpa.2020-0568-oa.Peer-Reviewed Original ResearchConceptsMpMRI-targeted biopsiesHigh-grade prostatic adenocarcinomaMultiparametric magnetic resonance imagingHG PCaRadical prostatectomyHigh-grade groupMagnetic resonance imagingPathologic stageRP outcomesProstatic adenocarcinomaGrade Group 2 diseaseResonance imagingGrade groupProstate-confined diseaseSeminal vesicle involvementLymph node metastasisHigher pathologic stageAdditional predictive valueLogistic regression modelsLinear millimeterNode metastasisRetrospective reviewAdverse outcomesTargeted biopsiesProstate biopsyAn independent assessment of an artificial intelligence system for prostate cancer detection shows strong diagnostic accuracy
Perincheri S, Levi AW, Celli R, Gershkovich P, Rimm D, Morrow JS, Rothrock B, Raciti P, Klimstra D, Sinard J. An independent assessment of an artificial intelligence system for prostate cancer detection shows strong diagnostic accuracy. Modern Pathology 2021, 34: 1588-1595. PMID: 33782551, PMCID: PMC8295034, DOI: 10.1038/s41379-021-00794-x.Peer-Reviewed Original ResearchConceptsMemorial Sloan-Kettering Cancer CenterCore biopsyPredictive valueDiagnostic accuracyProstate core needle biopsiesCore needle biopsySurgical pathology practiceNegative predictive valueProstate core biopsiesPositive predictive valueProstate cancer detectionStrong diagnostic accuracyPoor quality scansCancer detectionCancer CenterProstate biopsyLeading causeNeedle biopsyTransrectal approachProstate cancerProstatic adenocarcinomaProstate carcinomaBiopsyPathology practiceProstateHigh-Risk Human Papillomavirus Testing, Genotyping, and Histopathologic Follow-up in Women With Abnormal Glandular Cells on Papanicolaou Tests
Zuo T, Levi AW, Lin Q, Abi-Raad R, Adeniran AJ, Cai G. High-Risk Human Papillomavirus Testing, Genotyping, and Histopathologic Follow-up in Women With Abnormal Glandular Cells on Papanicolaou Tests. American Journal Of Clinical Pathology 2021, 156: 569-576. PMID: 33728437, DOI: 10.1093/ajcp/aqaa265.Peer-Reviewed Original ResearchConceptsPositive hrHPV resultsCervical intraepithelial neoplasiaHrHPV resultsHrHPV testingCIN2-3Intraepithelial neoplasiaPapanicolaou testCervical carcinomaHigh risk human papillomavirus statusHigh-risk human papillomavirus (hrHPV) testingHigh-grade cervical intraepithelial neoplasiaLow-grade cervical intraepithelial neoplasiaHuman papillomavirus testingHuman papillomavirus (HPV) statusManagement of patientsAbnormal glandular cellsHPV genotypesHPV subtypesAGC casesPap testFollow-upHistologic followHigh lesionsType 16Reactive changes
2020
Fine needle aspiration cytology of a neck mass
Gilani SM, Levi A. Fine needle aspiration cytology of a neck mass. Cytopathology 2020, 31: 622-623. PMID: 32569432, DOI: 10.1111/cyt.12878.Peer-Reviewed Original Research
2018
Prostate Cancer Genomic Classifier Relates More Strongly to Gleason Grade Group Than Prostate Imaging Reporting and Data System Score in Multiparametric Prostate Magnetic Resonance Imaging-ultrasound Fusion Targeted Biopsies
Martin DT, Ghabili K, Levi A, Humphrey PA, Sprenkle PC. Prostate Cancer Genomic Classifier Relates More Strongly to Gleason Grade Group Than Prostate Imaging Reporting and Data System Score in Multiparametric Prostate Magnetic Resonance Imaging-ultrasound Fusion Targeted Biopsies. Urology 2018, 125: 64-72. PMID: 30552940, DOI: 10.1016/j.urology.2018.12.001.Peer-Reviewed Original ResearchConceptsPI-RADSv2 scoreDecipher scoreProstate Imaging ReportingDecipher testingTargeted biopsiesRadical prostatectomyData System version 2 (PI-RADS v2) scoreFavorable intermediate-risk prostate cancerIntermediate-risk prostate cancer patientsGleason Grade Group 1Imaging ReportingIntermediate-risk prostate cancerMultivariable logistic regression analysisHigh-risk diseasePI-RADS 4Grade group 1Gleason grade groupProstate cancer patientsMultiparametric magnetic resonancePI-RADS scoreVersion 2 scoreData System scoreLogistic regression analysisSignificant differencesPI-RADS category
2017
Prostate zonal anatomy correlates with the detection of prostate cancer on multiparametric magnetic resonance imaging/ultrasound fusion–targeted biopsy in patients with a solitary PI-RADS v2–scored lesion
Syed JS, Nguyen KA, Nawaf CB, Bhagat AM, Huber S, Levi A, Humphrey P, Weinreb JC, Schulam PG, Sprenkle PC. Prostate zonal anatomy correlates with the detection of prostate cancer on multiparametric magnetic resonance imaging/ultrasound fusion–targeted biopsy in patients with a solitary PI-RADS v2–scored lesion. Urologic Oncology Seminars And Original Investigations 2017, 35: 542.e19-542.e24. PMID: 28501565, DOI: 10.1016/j.urolonc.2017.04.011.Peer-Reviewed Original ResearchConceptsPositive predictive valueProstate cancerGleason scoreSuspicious findingsMultiparametric magnetic resonance imagingProstate multiparametric magnetic resonance imagingPeripheral zoneHigh positive predictive valueFusion-targeted biopsyProstate Imaging ReportingPI-RADS v2Category 4 lesionsMagnetic resonance imagingProstate zonal anatomySystematic biopsyProstate biopsyFusion biopsySingle lesionPatientsBiopsyPredictive valueImaging ReportingResonance imagingLesionsCancerNegative Multiparametric Magnetic Resonance Imaging of the Prostate Predicts Absence of Clinically Significant Prostate Cancer on 12-Core Template Prostate Biopsy
Lu AJ, Syed JS, Nguyen KA, Nawaf CB, Rosoff J, Spektor M, Levi A, Humphrey PA, Weinreb JC, Schulam PG, Sprenkle PC. Negative Multiparametric Magnetic Resonance Imaging of the Prostate Predicts Absence of Clinically Significant Prostate Cancer on 12-Core Template Prostate Biopsy. Urology 2017, 105: 118-122. PMID: 28322902, DOI: 10.1016/j.urology.2017.01.048.Peer-Reviewed Original ResearchConceptsMultiparametric magnetic resonance imagingSignificant prostate cancerProstate cancerNegative predictive valueMagnetic resonance imagingSystematic TRUSNegative mpMRIProstate biopsyNegative multiparametric magnetic resonance imagingPredictive valueResonance imagingClinically Significant Prostate CancerDynamic contrast enhancement sequencesTemplate mapping biopsyPrior negative biopsySystematic prostate biopsyOverall cancer detectionProstate-specific antigenTemplate prostate biopsyProstate cancer detectionDiffusion-weighted imagingCancer detectionBiopsy naïveMapping biopsyProstate volume
2016
A Comparison of the Roche Cobas HPV Test With the Hybrid Capture 2 Test for the Detection of High-Risk Human Papillomavirus Genotypes
Levi AW, Bernstein JI, Hui P, Duch K, Schofield K, Chhieng DC. A Comparison of the Roche Cobas HPV Test With the Hybrid Capture 2 Test for the Detection of High-Risk Human Papillomavirus Genotypes. Archives Of Pathology & Laboratory Medicine 2016, 140: 153-7. PMID: 26910220, DOI: 10.5858/arpa.2015-0027-oa.Peer-Reviewed Original ResearchConceptsHigh-risk human papillomavirusHuman papillomavirus assaysCobas human papillomavirus (HPV) testHigh-risk human papillomavirus DNAHybrid Capture 2 testHuman papillomavirus testingHuman papillomavirus testHuman papillomavirus DNAHuman papillomavirusPapillomavirus DNAHigh-risk human papillomavirus genotypesRoche cobas HPV testRoche CobasSurePath preparationsThinPrep specimensDiscordant resultsHybrid captureCobas HPV TestHuman papillomavirus resultsLiquid-based cytology samplesHybrid Capture 2Human papillomavirus genotypesLiquid-based preparationsHPV testCapture 2
2015
Detection and Genotype of High-Risk Human Papillomavirus in Fine-Needle Aspirates of Patients With Metastatic Squamous Cell Carcinoma Is Helpful in Determining Tumor Origin
Baldassarri R, Aronberg R, Levi AW, Yarbrough WG, Kowalski D, Chhieng D. Detection and Genotype of High-Risk Human Papillomavirus in Fine-Needle Aspirates of Patients With Metastatic Squamous Cell Carcinoma Is Helpful in Determining Tumor Origin. American Journal Of Clinical Pathology 2015, 143: 694-700. PMID: 25873503, DOI: 10.1309/ajcpcza4pszcfhq4.Peer-Reviewed Original ResearchConceptsSquamous cell carcinomaCobas 4800 systemRoche cobas 4800 systemMetastatic squamous cell carcinomaFine needle aspiratesHuman papillomavirusHR-HPVCell carcinomaHPV 16High-risk (HR) HPVSquamous cell carcinoma originatingHigh-risk human papillomavirusNeck squamous cell carcinomaHPV 16/18 genotypesHR-HPV testingHR-HPV detectionHR-HPV resultsCorresponding histologic specimensLeft parapharyngeal massElectronic health recordsCarcinoma originatingMediastinal lymphadenopathyNegative HNSCCProspective studyHistologic material
2014
Methylation of Twelve CpGs in Human Papillomavirus Type 16 (HPV16) as an Informative Biomarker for the Triage of Women Positive for HPV16 Infection
Brandsma JL, Harigopal M, Kiviat NB, Sun Y, Deng Y, Zelterman D, Lizardi PM, Shabanova VS, Levi A, Yaping T, Hu X, Feng Q. Methylation of Twelve CpGs in Human Papillomavirus Type 16 (HPV16) as an Informative Biomarker for the Triage of Women Positive for HPV16 Infection. Cancer Prevention Research 2014, 7: 526-533. PMID: 24556390, DOI: 10.1158/1940-6207.capr-13-0354.Peer-Reviewed Original ResearchConceptsCervical intraepithelial neoplasiaCytology samplesCutoff scoreColposcopy of womenCervical cancer preventionManagement of womenDetection of CIN3Cervical cytology samplesHuman papillomavirus type 16Human papillomavirus type 16 DNAMethylation biomarkersPapillomavirus type 16Histologic severityIntraepithelial neoplasiaCervical cancerHPV16 infectionCancer preventionDNA bisulfite sequencingType 16Prognostic potentialAccurate biomarkersProspective biomarkersScreening testInformative biomarkersBiomarkersPrior High-Risk Human Papillomavirus Testing and Papanicolaou Test Results of 70 Invasive Cervical Carcinomas Diagnosed in 2012: Results of a Retrospective Multicenter Study
Zhao C, Li Z, Nayar R, Levi AW, Winkler BA, Moriarty AT, Barkan GA, Rao J, Miller F, Fan F, Zhou Z, Si Q, Fischer AH, Sturgis CD, Jing X, Marshall CB, Witt BL, Birdsong GG, Crothers BA. Prior High-Risk Human Papillomavirus Testing and Papanicolaou Test Results of 70 Invasive Cervical Carcinomas Diagnosed in 2012: Results of a Retrospective Multicenter Study. Archives Of Pathology & Laboratory Medicine 2014, 139: 184-8. PMID: 24694342, DOI: 10.5858/arpa.2014-0028-oa.Peer-Reviewed Original ResearchConceptsInvasive cervical cancerInvasive cervical carcinomaHrHPV testingCervical cancerCervical carcinomaHPV testingHistologic diagnosisPersistent high-risk human papillomavirus (HPV) infectionHigh-risk human papillomavirus (hrHPV) testingHigh-risk human papillomavirus infectionHigh-risk HPV testingPrimary HPV testingHuman papillomavirus testingHuman papillomavirus infectionRetrospective multicenter studyCervical epithelial lesionsCurrent screening guidelinesSquamous cell carcinomaHPV ratesPapillomavirus infectionMulticenter studyScreening guidelinesCell carcinomaPapanicolaou testSingle institution
2012
Increasing Cytotechnologist Workload Above 100 Slides per Day Using the BD FocalPoint GS Imaging System Negatively Affects Screening Performance
Levi AW, Galullo P, Gordy K, Mikolaiski N, Schofield K, Elsheikh TM, Harigopal M, Chhieng DC. Increasing Cytotechnologist Workload Above 100 Slides per Day Using the BD FocalPoint GS Imaging System Negatively Affects Screening Performance. American Journal Of Clinical Pathology 2012, 138: 811-815. PMID: 23161714, DOI: 10.1309/ajcptydd9g2ncumf.Peer-Reviewed Original ResearchMeSH KeywordsCarcinoma, Squamous CellCytodiagnosisDiagnostic ImagingFalse Negative ReactionsFemaleHumansImage Processing, Computer-AssistedMass ScreeningMedical Laboratory PersonnelQuality ControlSensitivity and SpecificityTime FactorsUterine Cervical DysplasiaUterine Cervical NeoplasmsVaginal SmearsWorkloadComparing Two Methods of Detection for Chlamydia trachomatis in Liquid-Based Papanicolaou Tests
Levi AW, Beckman D, Hui P, Schofield K, Harigopal M, Chhieng DC. Comparing Two Methods of Detection for Chlamydia trachomatis in Liquid-Based Papanicolaou Tests. American Journal Of Clinical Pathology 2012, 138: 236-240. PMID: 22904135, DOI: 10.1309/ajcp2b7xqtcnamjp.Peer-Reviewed Original ResearchConceptsHC2 assayBD Viper SystemXTR TechnologyPositive predictive valueDNA assaysLiquid-based Papanicolaou testsNucleic acidsC trachomatisChlamydia trachomatisPredictive valueHybrid Capture 2Reverse transcriptase-polymerase chain reactionChlamydia trachomatis testingTranscriptase-polymerase chain reactionViper SystemComparable specificityCapture 2Papanicolaou testChlamydial infectionCytology samplesMethod of detectionThinPrep specimensTrachomatisAssaysSensitivity rateThe Positive Impact of Simultaneous Implementation of the BD FocalPoint GS Imaging System and Lean Principles on the Operation of Gynecologic Cytology
Wong R, Levi AW, Harigopal M, Schofield K, Chhieng DC. The Positive Impact of Simultaneous Implementation of the BD FocalPoint GS Imaging System and Lean Principles on the Operation of Gynecologic Cytology. Archives Of Pathology & Laboratory Medicine 2012, 136: 183-9. PMID: 22288966, DOI: 10.5858/arpa.2011-0139-oa.Peer-Reviewed Original Research
2011
Implementation of FocalPoint GS location‐guided imaging system
Levi AW, Chhieng DC, Schofield K, Kowalski D, Harigopal M. Implementation of FocalPoint GS location‐guided imaging system. Cancer Cytopathology 2011, 120: 126-133. PMID: 22505211, DOI: 10.1002/cncy.20187.Peer-Reviewed Original ResearchConceptsASC-US casesHR-HPV positive rateLow-risk patient populationHigh-risk human papillomavirusASC-US interpretationsRoutine clinical settingPromising screening toolPap testHuman papillomavirusPapanicolaou testPatient populationBacterial vaginosisDrug AdministrationSIL ratioClinical settingScreening toolDiagnostic categoriesLaboratory information systemGynecologic cytologyPrimary screeningSignificant increaseFalse negative fractionPositive rateHigh percentageCurrent studyUse of high‐risk human papillomavirus testing in patients with low‐grade squamous intraepithelial lesions
Levi AW, Harigopal M, Hui P, Schofield K, Chhieng DC. Use of high‐risk human papillomavirus testing in patients with low‐grade squamous intraepithelial lesions. Cancer Cytopathology 2011, 119: 228-234. PMID: 21732551, DOI: 10.1002/cncy.20172.Peer-Reviewed Original ResearchConceptsLow-grade squamous intraepithelial lesionsHR-HPV testingHPV testingCIN 2Colposcopy rateHR-HPVRelative light units/cutoffCervical intraepithelial neoplasia 2High-risk human papillomavirusHR-HPV statusRLU/COIntraepithelial neoplasia 2Hybrid Capture 2Squamous intraepithelial lesionsCIN 1 lesionsLarge teaching hospitalNegative predictive valueLSIL cytologyHPV statusIntraepithelial lesionsCapture 2Human papillomavirusTeaching hospitalCytologic diagnosisSevere lesionsComparison of Affirm VPIII and Papanicolaou Tests in the Detection of Infectious Vaginitis
Levi AW, Harigopal M, Hui P, Schofield K, Chhieng DC. Comparison of Affirm VPIII and Papanicolaou Tests in the Detection of Infectious Vaginitis. American Journal Of Clinical Pathology 2011, 135: 442-447. PMID: 21350100, DOI: 10.1309/ajcp7tbn5vzuglzu.Peer-Reviewed Original ResearchConceptsAffirm VPIIIPap testPapanicolaou testRoutine Pap testRoutine Papanicolaou testCandida speciesSensitive diagnostic testInfectious vaginitisLarge academic institutionPatient populationTrichomonas infectionBacterial vaginosisCandida vaginitisG vaginalisT vaginalisStudy populationHigh incidenceDiagnostic testsMolecular testsPatientsVaginitisVaginalisMorphologic identificationVaginosisCandidiasis
2009
Prenatal diagnosis of an umbilical vein aneurysm: a case report.
Panda B, Stiller R, Iruretagoyena I, Levi A. Prenatal diagnosis of an umbilical vein aneurysm: a case report. Connecticut Medicine 2009, 73: 465-7. PMID: 19777980.Peer-Reviewed Original Research