Carlos Fernandez-Hernando, PhD
Associate Professor of Comparative Medicine and of Pathology
Research Interests
Pathology; Atherosclerosis
Research Organizations
Comparative Medicine: Integrative Cell Signaling & Neurobiology of Metabolism (ICSNM)
Center for RNA Science and Medicine
Vascular Biology and Therapeutics Program
Research Summary
We combine cell biology, genetics and mouse models to study lipid metabolism and cardiovascular related disorders. In particular, our research program aims to:
1. Identifying novel mechanisms by which cholesterol metabolism is regulated.
2. Assessing the contribution of non-coding RNA in regulating lipid metabolism.
3. Developing novel non-coding RNA based therapies for treating cardiovascular disorders.
Specialized Terms: Cholesterol homeostasis; Lipoprotein metabolism; Post-transcriptional regulation; microRNAs; Atherosclerosis; RNAi screening
Extensive Research Description
Our research aims to identify and characterize novel mechanisms by which cholesterol and lipoprotein metabolism is regulated. To date, most lipid and lipoprotein research has focused on alterations of protein coding genes, whereas the functions of non-coding RNAs remain largely unknown. Particular efforts are focused on microRNAs (miRNAs), a novel class of small non-coding RNAs that mediate port-transcriptional gene silencing. Using mouse models and cell culture studies, we will elucidate the molecular basis of the miRNA functions in regulating lipid metabolism and explore the potential of miRNAs as therapetic targets.
miRNAs have emerged as critical regulators of gene expression at the posttranscriptional level. miRNAs typically control the expression of their target genes by imperfect base pairing to the 3’ untranslated regions (3’UTR) of messenger RNAs (mRNAs) thereby inducing repression of the target mRNA. Bioinformatic predictions and experimental approaches indicate that a single miRNA may target more than a hundred mRNAs. Indeed, human miRNAs are predicted to control the activity of more than 60% of all protein-coding genes. This class of short (22 nucleotides) noncoding RNA molecules has been shown to participate in almost every cellular process investigated so far, and their dysregulation is observed in, and might underlie, different human pathologies including cancer, heart disease, and neurodegeneration. Very recently, we have demonstrated that miR-33, an intronic miRNA located within the SREBP-2 gene, plays important roles in the homeostatic regulation of cholesterol metabolism. miR-33 inhibits the expression of the ATP-binding cassette (ABC) transporter, ABCA1, thereby attenuating both cholesterol efflux to apoA1 and high-density lipoprotein (HDL) biogenesis. Conversely, silencing of miR-33 in vivo increased hepatic ABCA1 and plasma HDL. Because plasma HDL levels show a strong inverse correlation with atherosclerotic vascular disease, there has been intense interest in therapeutically targeting HDL and macrophage cholesterol efflux pathways. Our study suggests that antagonists of endogenous miR-33 may be a useful therapeutic strategy for enhancing ABCA1 expression and raising HDL levels in vivo. In addition, our recent preliminary data suggest that miR-33 also coordinates genes regulating fatty acid metabolism and insulin signaling. Therefore, we plan to continue investigating the potential relevance of miR-33 expression in metabolic syndrome. Moreover, we are working with other miRNAs involved in the regulation of cellular cholesterol homeostasis, and depending on the results, would pursue the most promising candidates in more detail.
A second major project is to characterize new genes involved in the regulation of cholesterol. A tightly controlled-but only partially characterized-network of cellular signaling and lipid transfer systems orchestrates the functional compartmentalization of cholesterol within and between tissues at the whole body level. Increased understanding of these processes and their integration at the organ systems level provides fundamental insights into the physiology of cholesterol metabolism. However several issues await further studies. For the most sterol transport processes, only a limited number of proteins that are involved have been identified and very little is known about cholesterol trafficking in many physiologically relevant cell types, such us hepatocytes, enterocytes or cells of the central nervous system. Future work will focus on determining the molecular mechanisms involved in the cholesterol metabolism in mammalian cells using functional genomic screens. Our current studies aim to identify new genes regulating low-density lipoprotein receptor activity and trafficking in human hepatic cell lines using a genome-wide RNA interference (RNAi) screens. Besides increasing our insights into the physiology of cholesterol trafficking, the information obtained should help to develop improved strategies for management of cholesterol-related pathologies.
- Role of Caveolin-1 in regulating lipoprotein metabolism and cardiovascular disorders.
- Regulation of lipid metabolism by microRNAs
- Identification of novel genes involved in the regulation cholesterol metabolism using genome-wide siRNAs screens
- Regulation of sterol metabolism by inflammation
Selected Publications
-
Identification of Golgi localized DHHC-acyl-transferase enzymes that catalyze the palmitoylation and function of endothelial nitric oxide synthase
Fernández-Hernando C, Fukata M, Bernatchez PN, Fukata Y, Lin MI, Bredt DS, Sessa WC. J Cell Biol. 2006; 174(3): 369-377 2006
-
Loss of Akt1 leads to severe atherosclerosis and occlusive coronary artery disease
Fernández-Hernando C, Ackah E, Yu J, Suárez Y, Murata T, Iwakiri Y, Prendergast J, Miao RQ, Birnbaum MJ and Sessa WC. Cell Metab. 2007; 6: 446-457 2007
-
Genetic evidence supporting a critical role of endothelial caveolin-1 during the progression of atherosclerosis.
Fernández-Hernando C#, Yu J, Suárez Y, Rahner C, Dávalos A, Lasunción MA and Sessa WC#. (# corresponding author). Cell Metab. 2009 Jul; 10: 48-54. 2009
-
MiR-33 contributes to the regulation of cholesterol homeostasis.
Rayner KJ, Suárez Y, Dávalos A, Parathath S, Fitzgerald ML, Tamehiro N, Fisher EA, Moore KJ, Fernández-Hernando C. MiR-33 contributes to the regulation of cholesterol homeostasis. Science (New York, N.Y.) 2010, 328:1570-3. 2010
-
miR-33a/b contribute to the regulation of fatty acid metabolism and insulin signaling.
Dávalos A, Goedeke L, Smibert P, Ramírez CM, Warrier NP, Andreo U, Cirera-Salinas D, Rayner K, Suresh U, Pastor-Pareja JC, Esplugues E, Fisher EA, Penalva LO, Moore KJ, Suárez Y, Lai EC, Fernández-Hernando C. miR-33a/b contribute to the regulation of fatty acid metabolism and insulin signaling. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108:9232-7. 2011
-
Long-term therapeutic silencing of miR-33 increases circulating triglyceride levels and hepatic lipid accumulation in mice.
Goedeke L, Salerno A, Ramírez CM, Guo L, Allen RM, Yin X, Langley SR, Esau C, Wanschel A, Fisher EA, Suárez Y, Baldán A, Mayr M, Fernández-Hernando C. Long-term therapeutic silencing of miR-33 increases circulating triglyceride levels and hepatic lipid accumulation in mice. EMBO Molecular Medicine 2014, 6:1133-41. 2014
-
MicroRNA-148a regulates LDL receptor and ABCA1 expression to control circulating lipoprotein levels.
Goedeke L, Rotllan N, Canfrán-Duque A, Aranda JF, Ramírez CM, Araldi E, Lin CS, Anderson NN, Wagschal A, de Cabo R, Horton JD, Lasunción MA, Näär AM, Suárez Y, Fernández-Hernando C. MicroRNA-148a regulates LDL receptor and ABCA1 expression to control circulating lipoprotein levels. Nature Medicine 2015, 21:1280-9. 2015
-
ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression.
Aryal B, Rotllan N, Araldi E, Ramírez CM, He S, Chousterman BG, Fenn AM, Wanschel A, Madrigal-Matute J, Warrier N, Martín-Ventura JL, Swirski FK, Suárez Y, Fernández-Hernando C. ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression. Nature Communications 2016, 7:12313. 2016
-
Lanosterol Modulates TLR4-Mediated Innate Immune Responses in Macrophages.
Araldi E, Fernández-Fuertes M, Canfrán-Duque A, Tang W, Cline GW, Madrigal-Matute J, Pober JS, Lasunción MA, Wu D, Fernández-Hernando C, Suárez Y. Lanosterol Modulates TLR4-Mediated Innate Immune Responses in Macrophages. Cell Reports 2017, 19:2743-2755. 2017
-
Macrophage deficiency of miR-21 promotes apoptosis, plaque necrosis, and vascular inflammation during atherogenesis.
Canfrán-Duque A, Rotllan N, Zhang X, Fernández-Fuertes M, Ramírez-Hidalgo C, Araldi E, Daimiel L, Busto R, Fernández-Hernando C, Suárez Y. Macrophage deficiency of miR-21 promotes apoptosis, plaque necrosis, and vascular inflammation during atherogenesis. EMBO Molecular Medicine 2017, 9:1244-1262. 2017
-
Genetic Dissection of the Impact of miR-33a and miR-33b during the Progression of Atherosclerosis.
Price NL, Rotllan N, Canfrán-Duque A, Zhang X, Pati P, Arias N, Moen J, Mayr M, Ford DA, Baldán Á, Suárez Y, Fernández-Hernando C. Genetic Dissection of the Impact of miR-33a and miR-33b during the Progression of Atherosclerosis. Cell Reports 2017, 21:1317-1330. 2017
-
Genetic Ablation of miR-33 Increases Food Intake, Enhances Adipose Tissue Expansion, and Promotes Obesity and Insulin Resistance.
Price NL, Singh AK, Rotllan N, Goedeke L, Wing A, Canfrán-Duque A, Diaz-Ruiz A, Araldi E, Baldán Á, Camporez JP, Suárez Y, Rodeheffer MS, Shulman GI, de Cabo R, Fernández-Hernando C. Genetic Ablation of miR-33 Increases Food Intake, Enhances Adipose Tissue Expansion, and Promotes Obesity and Insulin Resistance. Cell Reports 2018, 22:2133-2145. 2018
-
Absence of ANGPTL4 in adipose tissue improves glucose tolerance and attenuates atherogenesis.
Aryal B, Singh AK, Zhang X, Varela L, Rotllan N, Goedeke L, Chaube B, Camporez JP, Vatner DF, Horvath TL, Shulman GI, Suárez Y, Fernández-Hernando C. Absence of ANGPTL4 in adipose tissue improves glucose tolerance and attenuates atherogenesis. JCI Insight 2018, 3. 2018
Full List of PubMed Publications
- Price NL, Rotllan N, Zhang X, Canfran-Duque A, Nottoli T, Suarez Y, Fernandez-Hernando C: Specific Disruption of Abca1 Targeting Largely Mimics the Effects of miR-33 Knockout on Macrophage Cholesterol Efflux and Atherosclerotic Plaque Development. Circ Res. 2019 Feb 1; 2019 Feb 1. PMID: 30707082
- He S, Kahles F, Rattik S, Nairz M, McAlpine CS, Anzai A, Selgrade D, Fenn AM, Chan CT, Mindur JE, Valet C, Poller WC, Halle L, Rotllan N, Iwamoto Y, Wojtkiewicz GR, Weissleder R, Libby P, Fernández-Hernando C, Drucker DJ, Nahrendorf M, Swirski FK: Gut intraepithelial T cells calibrate metabolism and accelerate cardiovascular disease. Nature. 2019 Feb; 2019 Jan 30. PMID: 30700910
- Barwari T, Eminaga S, Mayr U, Lu R, Armstrong PC, Chan MV, Sahraei M, Fernández-Fuertes M, Moreau T, Barallobre-Barreiro J, Lynch M, Yin X, Schulte C, Baig F, Pechlaner R, Langley SR, Zampetaki A, Santer P, Weger M, Plasenzotti R, Schosserer M, Grillari J, Kiechl S, Willeit J, Shah AM, Ghevaert C, Warner TD, Fernández-Hernando C, Suárez Y, Mayr M: Inhibition of profibrotic microRNA-21 affects platelets and their releasate. JCI Insight. 2018 Nov 2; 2018 Nov 2. PMID: 30385722
- Zhang X, Sessa WC, Fernández-Hernando C: Endothelial Transcytosis of Lipoproteins in Atherosclerosis. Front Cardiovasc Med. 2018; 2018 Sep 25. PMID: 30320124
- Singh AK, Aryal B, Zhang X, Fan Y, Price NL, Suárez Y, Fernández-Hernando C: Posttranscriptional regulation of lipid metabolism by non-coding RNAs and RNA binding proteins. Semin Cell Dev Biol. 2018 Sep; 2017 Dec 6. PMID: 29183708
- Zhang F, Zarkada G, Han J, Li J, Dubrac A, Ola R, Genet G, Boyé K, Michon P, Künzel SE, Camporez JP, Singh AK, Fong GH, Simons M, Tso P, Fernández-Hernando C, Shulman GI, Sessa WC, Eichmann A: Lacteal junction zippering protects against diet-induced obesity. Science. 2018 Aug 10. PMID: 30093598
- Zhang X, Price NL, Fernández-Hernando C: Non-coding RNAs in lipid metabolism. Vascul Pharmacol. 2018 Jun 18; 2018 Jun 18. PMID: 29929012
- Misra A, Feng Z, Chandran RR, Kabir I, Rotllan N, Aryal B, Sheikh AQ, Ding L, Qin L, Fernández-Hernando C, Tellides G, Greif DM: Integrin beta3 regulates clonality and fate of smooth muscle-derived atherosclerotic plaque cells. Nat Commun. 2018 May 25; 2018 May 25. PMID: 29802249
- Fernández-Hernando C, Suárez Y: MicroRNAs in endothelial cell homeostasis and vascular disease. Curr Opin Hematol. 2018 May. PMID: 29547400
- Singh AK, Aryal B, Chaube B, Rotllan N, Varela L, Horvath TL, Suárez Y, Fernández-Hernando C: Brown adipose tissue derived ANGPTL4 controls glucose and lipid metabolism and regulates thermogenesis. Mol Metab. 2018 May; 2018 Mar 29. PMID: 29627378
- Lawan A, Min K, Zhang L, Canfran-Duque A, Jurczak MJ, Camporez JPG, Nie Y, Gavin TP, Shulman GI, Fernandez-Hernando C, Bennett AM: Skeletal Muscle-Specific Deletion of MKP-1 Reveals a p38 MAPK/JNK/Akt Signaling Node That Regulates Obesity-Induced Insulin Resistance. Diabetes. 2018 Apr; 2018 Jan 9. PMID: 29317435
- Aryal B, Singh AK, Zhang X, Varela L, Rotllan N, Goedeke L, Chaube B, Camporez JP, Vatner DF, Horvath TL, Shulman GI, Suárez Y, Fernández-Hernando C: Absence of ANGPTL4 in adipose tissue improves glucose tolerance and attenuates atherogenesis. JCI Insight. 2018 Mar 22; 2018 Mar 22. PMID: 29563332
- Miguel V, Cui JY, Daimiel L, Espinosa-Díez C, Fernández-Hernando C, Kavanagh TJ, Lamas S: The Role of MicroRNAs in Environmental Risk Factors, Noise-Induced Hearing Loss, and Mental Stress. Antioxid Redox Signal. 2018 Mar 20; 2017 Jun 30. PMID: 28562070
- Aranda JF, Rathjen S, Johannes L, Fernández-Hernando C: MiR-199a-5p attenuates retrograde transport and protects against toxin-induced inhibition of protein biosynthesis. Mol Cell Biol. 2018 Mar 19; 2018 Mar 19. PMID: 29555727
- Price NL, Singh AK, Rotllan N, Goedeke L, Wing A, Canfrán-Duque A, Diaz-Ruiz A, Araldi E, Baldán Á, Camporez JP, Suárez Y, Rodeheffer MS, Shulman GI, de Cabo R, Fernández-Hernando C: Genetic Ablation of miR-33 Increases Food Intake, Enhances Adipose Tissue Expansion, and Promotes Obesity and Insulin Resistance. Cell Rep. 2018 Feb 20. PMID: 29466739
- Price NL, Rotllan N, Canfrán-Duque A, Zhang X, Pati P, Arias N, Moen J, Mayr M, Ford DA, Baldán Á, Suárez Y, Fernández-Hernando C: Genetic Dissection of the Impact of miR-33a and miR-33b during the Progression of Atherosclerosis. Cell Rep. 2017 Oct 31. PMID: 29091769
- Canfrán-Duque A, Rotllan N, Zhang X, Fernández-Fuertes M, Ramírez-Hidalgo C, Araldi E, Daimiel L, Busto R, Fernández-Hernando C, Suárez Y: Macrophage deficiency of miR-21 promotes apoptosis, plaque necrosis, and vascular inflammation during atherogenesis. EMBO Mol Med. 2017 Sep. PMID: 28674080
- Sivarapatna A, Ghaedi M, Xiao Y, Han E, Aryal B, Zhou J, Fernandez-Hernando C, Qyang Y, Hirschi KK, Niklason LE: Engineered Microvasculature in PDMS Networks Using Endothelial Cells Derived from Human Induced Pluripotent Stem Cells. Cell Transplant. 2017 Aug. PMID: 28901188
- Kim JD, Toda C, Ramírez CM, Fernández-Hernando C, Diano S: Hypothalamic Ventromedial Lin28a Enhances Glucose Metabolism in Diet-Induced Obesity. Diabetes. 2017 Aug; 2017 May 26. PMID: 28550108
- Araldi E, Fernández-Fuertes M, Canfrán-Duque A, Tang W, Cline GW, Madrigal-Matute J, Pober JS, Lasunción MA, Wu D, Fernández-Hernando C, Suárez Y: Lanosterol Modulates TLR4-Mediated Innate Immune Responses in Macrophages. Cell Rep. 2017 Jun 27. PMID: 28658622
- Aryal B, Singh AK, Rotllan N, Price N, Fernández-Hernando C: MicroRNAs and lipid metabolism. Curr Opin Lipidol. 2017 Jun. PMID: 28333713
- Zhang X, Fernández-Hernando C: MiR-33 regulation of stretch-induced intimal hyperplasia in vein grafts. Cardiovasc Res. 2017 Apr 1. PMID: 28339676
- Zhang X, Fernández-Hernando C: miR-33 Regulation of Adaptive Fibrotic Response in Cardiac Remodeling. Circ Res. 2017 Mar 3. PMID: 28254794
- Willeit P, Skroblin P, Moschen AR, Yin X, Kaudewitz D, Zampetaki A, Barwari T, Whitehead M, Ramírez CM, Goedeke L, Rotllan N, Bonora E, Hughes AD, Santer P, Fernández-Hernando C, Tilg H, Willeit J, Kiechl S, Mayr M: Circulating MicroRNA-122 Is Associated With the Risk of New-Onset Metabolic Syndrome and Type 2 Diabetes. Diabetes. 2017 Feb; 2016 Nov 29. PMID: 27899485
- Suárez Y, Fernández-Hernando C: Preface to: "microRNAs in lipid/energy metabolism and cardiometabolic disease". Biochim Biophys Acta. 2016 Dec; 2016 Jul 7. PMID: 27396679
- Goedeke L, Wagschal A, Fernández-Hernando C, Näär AM: miRNA regulation of LDL-cholesterol metabolism. Biochim Biophys Acta. 2016 Dec; 2016 Mar 9. PMID: 26968099
- Price NL, Fernández-Hernando C: miRNA regulation of white and brown adipose tissue differentiation and function. Biochim Biophys Acta. 2016 Dec; 2016 Feb 16. PMID: 26898181
- Baldán Á, Fernández-Hernando C: Truths and controversies concerning the role of miRNAs in atherosclerosis and lipid metabolism. Curr Opin Lipidol. 2016 Dec. PMID: 27755115
- Kraehling JR, Chidlow JH, Rajagopal C, Sugiyama MG, Fowler JW, Lee MY, Zhang X, Ramírez CM, Park EJ, Tao B, Chen K, Kuruvilla L, Larriveé B, Folta-Stogniew E, Ola R, Rotllan N, Zhou W, Nagle MW, Herz J, Williams KJ, Eichmann A, Lee WL, Fernández-Hernando C, Sessa WC: Genome-wide RNAi screen reveals ALK1 mediates LDL uptake and transcytosis in endothelial cells. Nat Commun. 2016 Nov 21; 2016 Nov 21. PMID: 27869117
- Willeit P, Skroblin P, Kiechl S, Fernández-Hernando C, Mayr M: Liver microRNAs: potential mediators and biomarkers for metabolic and cardiovascular disease? Eur Heart J. 2016 Nov 14; 2016 Apr 20. PMID: 27099265
- Du W, Wong C, Song Y, Shen H, Mori D, Rotllan N, Price N, Dobrian AD, Meng H, Kleinstein SH, Fernandez-Hernando C, Goldstein DR: Age-associated vascular inflammation promotes monocytosis during atherogenesis. Aging Cell. 2016 Aug; 2016 May 2. PMID: 27135421
- Aryal B, Rotllan N, Araldi E, Ramírez CM, He S, Chousterman BG, Fenn AM, Wanschel A, Madrigal-Matute J, Warrier N, Martín-Ventura JL, Swirski FK, Suárez Y, Fernández-Hernando C: ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression. Nat Commun. 2016 Jul 27; 2016 Jul 27. PMID: 27460411
- Canfrán-Duque A, Lin CS, Goedeke L, Suárez Y, Fernández-Hernando C: Micro-RNAs and High-Density Lipoprotein Metabolism. Arterioscler Thromb Vasc Biol. 2016 Jun; 2016 Apr 14. PMID: 27079881
- Ulrich V, Rotllan N, Araldi E, Luciano A, Skroblin P, Abonnenc M, Perrotta P, Yin X, Bauer A, Leslie KL, Zhang P, Aryal B, Montgomery RL, Thum T, Martin K, Suarez Y, Mayr M, Fernandez-Hernando C, Sessa WC: Chronic miR-29 antagonism promotes favorable plaque remodeling in atherosclerotic mice. EMBO Mol Med. 2016 Jun; 2016 Jun 1. PMID: 27137489
- Pauta M, Rotllan N, Fernández-Hernando A, Langhi C, Ribera J, Lu M, Boix L, Bruix J, Jimenez W, Suárez Y, Ford DA, Baldán A, Birnbaum MJ, Morales-Ruiz M, Fernández-Hernando C: Akt-mediated foxo1 inhibition is required for liver regeneration. Hepatology. 2016 May; 2015 Dec 18. PMID: 26473496
- Liu D, Lei L, Desir M, Huang Y, Cleman J, Jiang W, Fernandez-Hernando C, Di Lorenzo A, Sessa WC, Giordano FJ: Smooth Muscle Hypoxia-Inducible Factor 1α Links Intravascular Pressure and Atherosclerosis--Brief Report. Arterioscler Thromb Vasc Biol. 2016 Mar; 2016 Jan 21. PMID: 26800562
- Rotllan N, Price N, Pati P, Goedeke L, Fernández-Hernando C: microRNAs in lipoprotein metabolism and cardiometabolic disorders. Atherosclerosis. 2016 Mar; 2016 Jan 18. PMID: 26828754
- Price NL, Holtrup B, Kwei SL, Wabitsch M, Rodeheffer M, Bianchini L, Suárez Y, Fernández-Hernando C: SREBP-1c/MicroRNA 33b Genomic Loci Control Adipocyte Differentiation. Mol Cell Biol. 2016 Feb 1; 2016 Feb 1. PMID: 26830228
- Chamorro-Jorganes A, Lee MY, Araldi E, Landskroner-Eiger S, Fernández-Fuertes M, Sahraei M, Quiles Del Rey M, van Solingen C, Yu J, Fernández-Hernando C, Sessa WC, Suárez Y: VEGF-Induced Expression of miR-17-92 Cluster in Endothelial Cells Is Mediated by ERK/ELK1 Activation and Regulates Angiogenesis. Circ Res. 2016 Jan 8; 2015 Oct 15. PMID: 26472816
- Goedeke L, Rotllan N, Ramírez CM, Aranda JF, Canfrán-Duque A, Araldi E, Fernández-Hernando A, Langhi C, de Cabo R, Baldán Á, Suárez Y, Fernández-Hernando C: miR-27b inhibits LDLR and ABCA1 expression but does not influence plasma and hepatic lipid levels in mice. Atherosclerosis. 2015 Dec. PMID: 26520906
- Espinosa-Diez C, Fierro-Fernández M, Sánchez-Gómez F, Rodríguez-Pascual F, Alique M, Ruiz-Ortega M, Beraza N, Martínez-Chantar ML, Fernández-Hernando C, Lamas S: Targeting of Gamma-Glutamyl-Cysteine Ligase by miR-433 Reduces Glutathione Biosynthesis and Promotes TGF-β-Dependent Fibrogenesis. Antioxid Redox Signal. 2015 Nov 10; 2015 Jan 9. PMID: 25353619
- Kim J, Yoon H, Horie T, Burchett JM, Restivo JL, Rotllan N, Ramírez CM, Verghese PB, Ihara M, Hoe HS, Esau C, Fernández-Hernando C, Holtzman DM, Cirrito JR, Ono K, Kim J: microRNA-33 Regulates ApoE Lipidation and Amyloid-β Metabolism in the Brain. J Neurosci. 2015 Nov 4. PMID: 26538644
- Wagschal A, Najafi-Shoushtari SH, Wang L, Goedeke L, Sinha S, deLemos AS, Black JC, Ramírez CM, Li Y, Tewhey R, Hatoum I, Shah N, Lu Y, Kristo F, Psychogios N, Vrbanac V, Lu YC, Hla T, de Cabo R, Tsang JS, Schadt E, Sabeti PC, Kathiresan S, Cohen DE, Whetstine J, Chung RT, Fernández-Hernando C, Kaplan LM, Bernards A, Gerszten RE, Näär AM: Genome-wide identification of microRNAs regulating cholesterol and triglyceride homeostasis. Nat Med. 2015 Nov; 2015 Oct 26. PMID: 26501192
- Goedeke L, Rotllan N, Canfrán-Duque A, Aranda JF, Ramírez CM, Araldi E, Lin CS, Anderson NN, Wagschal A, de Cabo R, Horton JD, Lasunción MA, Näär AM, Suárez Y, Fernández-Hernando C: MicroRNA-148a regulates LDL receptor and ABCA1 expression to control circulating lipoprotein levels. Nat Med. 2015 Nov; 2015 Oct 5. PMID: 26437365
- Aranda JF, Canfrán-Duque A, Goedeke L, Suárez Y, Fernández-Hernando C: The miR-199-dynamin regulatory axis controls receptor-mediated endocytosis. J Cell Sci. 2015 Sep 1; 2015 Jul 10. PMID: 26163491
- Daimiel-Ruiz L, Klett-Mingo M, Konstantinidou V, Micó V, Aranda JF, García B, Martínez-Botas J, Dávalos A, Fernández-Hernando C, Ordovás JM: Dietary lipids modulate the expression of miR-107, a miRNA that regulates the circadian system. Mol Nutr Food Res. 2015 Sep. PMID: 26387852
- Price NL, Fernández-Hernando C: Novel Role of miR-33 in Regulating of Mitochondrial Function. Circ Res. 2015 Jul 17. PMID: 26185207
- García R, Nistal JF, Merino D, Price NL, Fernández-Hernando C, Beaumont J, González A, Hurlé MA, Villar AV: p-SMAD2/3 and DICER promote pre-miR-21 processing during pressure overload-associated myocardial remodeling. Biochim Biophys Acta. 2015 Jul; 2015 Apr 15. PMID: 25887159
- Rotllan N, Wanschel AC, Fernández-Hernando A, Salerno AG, Offermanns S, Sessa WC, Fernández-Hernando C: Genetic Evidence Supports a Major Role for Akt1 in VSMCs During Atherogenesis. Circ Res. 2015 May 22; 2015 Apr 13. PMID: 25868464
- Goodwin JE, Zhang X, Rotllan N, Feng Y, Zhou H, Fernández-Hernando C, Yu J, Sessa WC: Endothelial glucocorticoid receptor suppresses atherogenesis--brief report. Arterioscler Thromb Vasc Biol. 2015 Apr; 2015 Feb 19. PMID: 25810297
- Rotllan N, Chamorro-Jorganes A, Araldi E, Wanschel AC, Aryal B, Aranda JF, Goedeke L, Salerno AG, Ramírez CM, Sessa WC, Suárez Y, Fernández-Hernando C: Hematopoietic Akt2 deficiency attenuates the progression of atherosclerosis. FASEB J. 2015 Feb; 2014 Nov 12. PMID: 25392271
- Goedeke L, Fernández-Hernando C: MicroRNAs: a connection between cholesterol metabolism and neurodegeneration. Neurobiol Dis. 2014 Dec; 2014 Jun 5. PMID: 24907491
- Sala F, Aranda JF, Rotllan N, Ramírez CM, Aryal B, Elia L, Condorelli G, Catapano AL, Fernández-Hernando C, Norata GD: MiR-143/145 deficiency attenuates the progression of atherosclerosis in Ldlr-/-mice. Thromb Haemost. 2014 Oct; 2014 Jul 10. PMID: 25008143
- Goedeke L, Salerno A, Ramírez CM, Guo L, Allen RM, Yin X, Langley SR, Esau C, Wanschel A, Fisher EA, Suárez Y, Baldán A, Mayr M, Fernández-Hernando C: Long-term therapeutic silencing of miR-33 increases circulating triglyceride levels and hepatic lipid accumulation in mice. EMBO Mol Med. 2014 Sep. PMID: 25038053
- Canfrán-Duque A, Ramírez CM, Goedeke L, Lin CS, Fernández-Hernando C: microRNAs and HDL life cycle. Cardiovasc Res. 2014 Aug 1; 2014 Jun 3. PMID: 24895349
- Goedeke L, Aranda JF, Fernández-Hernando C: microRNA regulation of lipoprotein metabolism. Curr Opin Lipidol. 2014 Aug. PMID: 24978143
- Fernández-Hernando C: Antiatherogenic properties of high-density lipoprotein-enriched microRNAs. Arterioscler Thromb Vasc Biol. 2014 Jun; 2014 Apr 24. PMID: 24764456
- Ramírez CM, Lin CS, Abdelmohsen K, Goedeke L, Yoon JH, Madrigal-Matute J, Martin-Ventura JL, Vo DT, Uren PJ, Penalva LO, Gorospe M, Fernández-Hernando C: RNA binding protein HuR regulates the expression of ABCA1. J Lipid Res. 2014 Jun; 2014 Apr 11. PMID: 24729624
- Aryal B, Rotllan N, Fernández-Hernando C: Noncoding RNAs and atherosclerosis. Curr Atheroscler Rep. 2014 May. PMID: 24623179
- Fan HC, Fernández-Hernando C, Lai JH: Protein kinase C isoforms in atherosclerosis: pro- or anti-inflammatory? Biochem Pharmacol. 2014 Mar 15; 2014 Jan 16. PMID: 24440741
- Albinsson S, Skoura A, Yu J, DiLorenzo A, Fernández-Hernando C, Offermanns S, Miano JM, Sessa WC: Smooth muscle miRNAs are critical for post-natal regulation of blood pressure and vascular function. PLoS One. 2011 Apr 22; 2011 Apr 22. PMID: 21526127
- Dávalos A, Fernández-Hernando C, Sowa G, Derakhshan B, Lin MI, Lee JY, Zhao H, Luo R, Colangelo C, Sessa WC: Quantitative proteomics of caveolin-1-regulated proteins: characterization of polymerase i and transcript release factor/CAVIN-1 IN endothelial cells. Mol Cell Proteomics. 2010 Oct; 2010 Jun 28. PMID: 20585024
- Fernández-Hernando C, Yu J, Dávalos A, Prendergast J, Sessa WC: Endothelial-specific overexpression of caveolin-1 accelerates atherosclerosis in apolipoprotein E-deficient mice. Am J Pathol. 2010 Aug; 2010 Jun 25. PMID: 20581061
- Fernández-Hernando C, József L, Jenkins D, Di Lorenzo A, Sessa WC: Absence of Akt1 reduces vascular smooth muscle cell migration and survival and induces features of plaque vulnerability and cardiac dysfunction during atherosclerosis. Arterioscler Thromb Vasc Biol. 2009 Dec; 2009 Sep 17. PMID: 19762778
- Yu J, Fernández-Hernando C, Suarez Y, Schleicher M, Hao Z, Wright PL, DiLorenzo A, Kyriakides TR, Sessa WC: Reticulon 4B (Nogo-B) is necessary for macrophage infiltration and tissue repair. Proc Natl Acad Sci U S A. 2009 Oct 13; 2009 Sep 25. PMID: 19805174
- Harrison KD, Miao RQ, Fernandez-Hernándo C, Suárez Y, Dávalos A, Sessa WC: Nogo-B receptor stabilizes Niemann-Pick type C2 protein and regulates intracellular cholesterol trafficking. Cell Metab. 2009 Sep. PMID: 19723497
- Di Lorenzo A, Fernández-Hernando C, Cirino G, Sessa WC: Akt1 is critical for acute inflammation and histamine-mediated vascular leakage. Proc Natl Acad Sci U S A. 2009 Aug 25; 2009 Jul 21. PMID: 19622728
- Fernández-Hernando C, Yu J, Suárez Y, Rahner C, Dávalos A, Lasunción MA, Sessa WC: Genetic evidence supporting a critical role of endothelial caveolin-1 during the progression of atherosclerosis. Cell Metab. 2009 Jul. PMID: 19583953
- Suárez Y, Fernández-Hernando C, Yu J, Gerber SA, Harrison KD, Pober JS, Iruela-Arispe ML, Merkenschlager M, Sessa WC: Dicer-dependent endothelial microRNAs are necessary for postnatal angiogenesis. Proc Natl Acad Sci U S A. 2008 Sep 16; 2008 Sep 8. PMID: 18779589
- Schleicher M, Shepherd BR, Suarez Y, Fernandez-Hernando C, Yu J, Pan Y, Acevedo LM, Shadel GS, Sessa WC: Prohibitin-1 maintains the angiogenic capacity of endothelial cells by regulating mitochondrial function and senescence. J Cell Biol. 2008 Jan 14. PMID: 18195103
- Wang Z, Liu B, Wang P, Dong X, Fernandez-Hernando C, Li Z, Hla T, Li Z, Claffey K, Smith JD, Wu D: Phospholipase C beta3 deficiency leads to macrophage hypersensitivity to apoptotic induction and reduction of atherosclerosis in mice. J Clin Invest. 2008 Jan. PMID: 18079968
- Fernández-Hernando C, Ackah E, Yu J, Suárez Y, Murata T, Iwakiri Y, Prendergast J, Miao RQ, Birnbaum MJ, Sessa WC: Loss of Akt1 leads to severe atherosclerosis and occlusive coronary artery disease. Cell Metab. 2007 Dec. PMID: 18054314
- Bernatchez PN, Acevedo L, Fernandez-Hernando C, Murata T, Chalouni C, Kim J, Erdjument-Bromage H, Shah V, Gratton JP, McNally EM, Tempst P, Sessa WC: Myoferlin regulates vascular endothelial growth factor receptor-2 stability and function. J Biol Chem. 2007 Oct 19; 2007 Aug 16. PMID: 17702744
- Suárez Y, Fernández-Hernando C, Pober JS, Sessa WC: Dicer dependent microRNAs regulate gene expression and functions in human endothelial cells. Circ Res. 2007 Apr 27; 2007 Mar 22. PMID: 17379831
- Fernández-Hernando C, Fukata M, Bernatchez PN, Fukata Y, Lin MI, Bredt DS, Sessa WC: Identification of Golgi-localized acyl transferases that palmitoylate and regulate endothelial nitric oxide synthase. J Cell Biol. 2006 Jul 31; 2006 Jul 24. PMID: 16864653