The Yale Larynx Lab chosen as cover of Oncotarget, Vol 11, Issue 35

The in vivo pre- or post- topical application of BAY 11-7082 prevents the acidic bile-induced deregulation of cancer-related miRNA markers in 10-day exposed murine HM. (A) Columns of graphs created by Graph Pad Prism software 7.0 depict oncogenic miRNA phenotype (“oncomirs” and “tumor suppressor” miRNAs) in 10-day (two times per day) exposed HM to acidic bile (pH 3.0) vs. saline-DMSO treated HM, pre-BAY 11-7082 + acidic bile (pH 3.0) vs. acidic bile alone treated HM, and acidic bile (pH 3.0) + post-BAY 11-7082 vs. acidic bile alone treated HM (miRNA levels of each target miRNA marker were normalized to RNU6; by real-time qPCR analysis). We observe that pre- or post- application of BAY 11-7082 reverses the acidic bile-induced miRNA phenotype. (B) Graphs, created by Graph Pad Prism 7.0 software, reveal miRNA levels (normalized to RNU6) for each analyzed miRNA marker, comparing HM exposed to acidic bile alone, application of BAY 11-7082 before or after acidic bile and saline-DMSO, by real-time qPCR analysis (p value < 0.05; by t test; multiple comparisons by Holm-Sidak; data obtained from four analyzed samples).