Methods used to generate a composite map from a single group of subjects.
Generate an .msb setup file as described in the Multisubject Manual Page , an example setup file can be seen here. This procedure can be run with two programs within the BioImageSuite package: either the BrainRegister program or the DualMultiSubject program. The two programs are the same except for the fact that the DualMultiSubject program includes two MultiSubject tabs instead of only one MultiSubject tab with BrainRegister. This extra MultiSubject tab is helpful when trying to compare two groups of subjects (as seen in the next set of instructions)
- Choose brainregister or dualmultisubject from the BioImageSuite main menu. Three windows will appear: a Transform Viewer, a Reference Viewer and a BrainRegister menu bar. On the BrainRegister menu bar choose (Multisubject | Base). A new MultiSubject Tool window will appear.
- On the new MultiSubject Tool window choose (Setup | Load Setup).
- Choose your generated .msb file and click Open.
- On the MultiSubject Tool window choose the Results Tab.
- Choose your task of interest from the list of tasks then click Change Current Task. If your task of interest is the first task the program will warn you that the new task is the same as the old task. The status bar at the bottom of the window will create a blue bar increasing in length as each subject's filename is renamed to the new task. The word Done will appear next to the status bar when the renaming is complete. If you toggle back to the Subject Tab, you should see your new task in the Functional Image Filename.
- On the MultiSubject Tool window click (Images | Load All Images). The status bar at the bottom of the window will create a blue bar increasing in length as the number of loaded subjects increases. The word Done will appear next to the status bar when the loading is complete.
- On the MultiSubject Tool window click Compute Average Functional. The status bar at the bottom of the window will create a blue bar increasing in length as the number of subjects computed into the average increases. The word Done will appear next to the status bar when the computation is complete.
- On the MultiSubject Tool window the bottom portion is labeled Output Overlay. There are several options to choose that will change the output image using three separate toggle buttons: Output Viewer, Base and Function. The Output Viewer button chooses which window (Reference or Transform) in which the output will be displayed. The Base button allows the user to choose the anatomical image on which to overlay to results. Finally, the Function button allows the user to choose between average function, t-score functional or individual functional. For purposes of this demonstration choose Output Viewer to be Transform, Base to be Reference Image and Function to be T-score of Average Functional.
- Choose Threshold (See steps 4-6 on the Overlay Common Space Tmaps onto Reference Image Instructions for how to choose a threshold).
- Click Create Overlay, the program will display the results in the chosen output viewer.