2025
Implications for OLE RNA as a natural integral membrane RNA.
Lyon S, Breaker R. Implications for OLE RNA as a natural integral membrane RNA. RNA 2025, rna.080489.125. PMID: 40393771, DOI: 10.1261/rna.080489.125.Peer-Reviewed Original ResearchOLE RNARNA world organismsGram-positive bacterial speciesCellular stress responseEmergence of proteinsBacterial hostsLocalized to cell membranesBilayer of membranesRibonucleoprotein complexMaster regulatorsBacterial speciesBiochemical functionsEvolutionary emergenceStress responseRNANoncoding RNAsRNA polymersCell membraneUnusual classPhospholipid bilayersRibonucleoproteinMembraneProteinSpeciesOrnateEvidence that ribosomal protein bS21 is a component of the OLE ribonucleoprotein complex
Wencker F, Lyon S, Breaker R. Evidence that ribosomal protein bS21 is a component of the OLE ribonucleoprotein complex. RNA Biology 2025, 22: 1-14. PMID: 40322971, PMCID: PMC12054373, DOI: 10.1080/15476286.2025.2491842.Peer-Reviewed Original ResearchConceptsOLE RNARNA binding sitesRNP complexesStructured noncoding RNAsBinding sitesExcess Mg<sup>2+</sup>Unfavourable growth conditionsMutational disruptionEthanol stressGrowth defectK-turnRibonucleoprotein complexBiologically relevant componentsBiochemical functionsCarbon/energy sourceBS21RNAProtein ANoncoding RNAsConformational changesGrowth inhibitionNcRNAsGrowth conditionsRibonucleoproteinBacillota
2024
Bioinformatic prediction of proteins relevant to functions of the bacterial OLE ribonucleoprotein complex
Fernando C, Breaker R. Bioinformatic prediction of proteins relevant to functions of the bacterial OLE ribonucleoprotein complex. MSphere 2024, 9: e00159-24. PMID: 38771028, PMCID: PMC11332333, DOI: 10.1128/msphere.00159-24.Peer-Reviewed Original ResearchOLE RNANoncoding RNA classesRNP complexesRNA classesSequence conservationProtein partnersBiochemical functionsDiverse stress conditionsProtein binding partnersEmergence of proteinsPhylogenetic profilesRibonucleoprotein complexSpore formationBacterial speciesCellular stressExtremophilic bacteriaProtein componentsRibonucleoproteinGenetic disruptionRNATransport proteinsRelevant to functionBioinformatics predictionGram-positivePrimitive organismsDisruption of the bacterial OLE RNP complex impairs growth on alternative carbon sources
Lyon S, Wencker F, Fernando C, Harris K, Breaker R. Disruption of the bacterial OLE RNP complex impairs growth on alternative carbon sources. PNAS Nexus 2024, 3: pgae075. PMID: 38415217, PMCID: PMC10898510, DOI: 10.1093/pnasnexus/pgae075.Peer-Reviewed Original ResearchRNP complexesMinimal mediumWild-type cellsAlternative carbon sourcesUnfavorable growth conditionsOLE RNASuppressor selectionDiverse stressesCarbon/energy sourceProtein secretionCarbon sourceGenetic disruptionCellular adaptationNoncoding RNAsFunctional linkRNAGrowth conditionsRibonucleoproteinImpaired growthPhosphate homeostasisFundamental processesHomeostasisShort-chain alcoholsElevated MgCarbon/energy
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