2017
Synaptic Vesicle Clusters at Synapses: A Distinct Liquid Phase?
Milovanovic D, De Camilli P. Synaptic Vesicle Clusters at Synapses: A Distinct Liquid Phase? Neuron 2017, 93: 995-1002. PMID: 28279363, PMCID: PMC5347463, DOI: 10.1016/j.neuron.2017.02.013.Peer-Reviewed Original Research
2004
Impaired PtdIns(4,5)P2 synthesis in nerve terminals produces defects in synaptic vesicle trafficking
Paolo G, Moskowitz HS, Gipson K, Wenk MR, Voronov S, Obayashi M, Flavell R, Fitzsimonds RM, Ryan TA, Camilli P. Impaired PtdIns(4,5)P2 synthesis in nerve terminals produces defects in synaptic vesicle trafficking. Nature 2004, 431: 415-422. PMID: 15386003, DOI: 10.1038/nature02896.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsBiological TransportCells, CulturedClathrinElectric ConductivityEndocytosisExocytosisGene DeletionKineticsMiceMice, KnockoutNeuronsPhosphatidylinositol 4,5-DiphosphatePhosphatidylinositol PhosphatesPhosphotransferases (Alcohol Group Acceptor)Presynaptic TerminalsSynaptic TransmissionSynaptic VesiclesConceptsClathrin coat dynamicsSynaptic vesicle cycleSynaptic vesicle exocytosisSynaptic vesicle traffickingSecond messenger moleculesEarly postnatal lethalityEndocytic intermediatesVesicle traffickingMembrane proteinsVesicle cycleVesicle exocytosisPostnatal lethalityCell regulationRecycling kineticsMessenger moleculesBiochemical studiesSynaptic defectsDirect interactionImportant functionsCritical roleMultiple stepsReleasable poolRegulationNerve terminalsDephosphorylation
2001
Chronic Blockade of Glutamate Receptors Enhances Presynaptic Release and Downregulates the Interaction between Synaptophysin-Synaptobrevin–Vesicle-Associated Membrane Protein 2
Bacci A, Coco S, Pravettoni E, Schenk U, Armano S, Frassoni C, Verderio C, De Camilli P, Matteoli M. Chronic Blockade of Glutamate Receptors Enhances Presynaptic Release and Downregulates the Interaction between Synaptophysin-Synaptobrevin–Vesicle-Associated Membrane Protein 2. Journal Of Neuroscience 2001, 21: 6588-6596. PMID: 11517248, PMCID: PMC6763110, DOI: 10.1523/jneurosci.21-17-06588.2001.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedDown-RegulationEndocytosisExcitatory Amino Acid AntagonistsExcitatory Postsynaptic PotentialsExocytosisHippocampusMacromolecular SubstancesMembrane ProteinsNeuronsPatch-Clamp TechniquesPresynaptic TerminalsProtein BindingRatsReceptors, GlutamateReceptors, N-Methyl-D-AspartateR-SNARE ProteinsSynaptic TransmissionSynaptic VesiclesSynaptophysinTetrodotoxinConceptsGlutamate receptorsSynapse formationSynaptic vesicle recyclingMiniature EPSC frequencyGlutamate receptor blockersGlutamatergic nerve terminalsNumber of synapsesUptake of antibodiesVesicle recyclingChronic blockadeReceptor blockersEPSC frequencyPresynaptic releaseNerve terminalsHippocampal neuronsPresynaptic functionPostsynaptic functionChronic exposureChronic presenceInterneuronal signalingNeuronal culturesNeuronal circuitsMembrane protein 2Intracellular perfusionPrimary cultures
1999
Tetanus Toxin Blocks the Exocytosis of Synaptic Vesicles Clustered at Synapses But Not of Synaptic Vesicles in Isolated Axons
Verderio C, Coco S, Bacci A, Rossetto O, De Camilli P, Montecucco C, Matteoli M. Tetanus Toxin Blocks the Exocytosis of Synaptic Vesicles Clustered at Synapses But Not of Synaptic Vesicles in Isolated Axons. Journal Of Neuroscience 1999, 19: 6723-6732. PMID: 10436029, PMCID: PMC6782867, DOI: 10.1523/jneurosci.19-16-06723.1999.Peer-Reviewed Original ResearchThe Interaction of Epsin and Eps15 with the Clathrin Adaptor AP-2 Is Inhibited by Mitotic Phosphorylation and Enhanced by Stimulation-dependent Dephosphorylation in Nerve Terminals*
Chen H, Slepnev V, Di Fiore P, De Camilli P. The Interaction of Epsin and Eps15 with the Clathrin Adaptor AP-2 Is Inhibited by Mitotic Phosphorylation and Enhanced by Stimulation-dependent Dephosphorylation in Nerve Terminals*. Journal Of Biological Chemistry 1999, 274: 3257-3260. PMID: 9920862, DOI: 10.1074/jbc.274.6.3257.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Protein Complex alpha SubunitsAdaptor Proteins, Signal TransducingAdaptor Proteins, Vesicular TransportAnimalsBase SequenceCalcium-Binding ProteinsCarrier ProteinsCell LineDNA PrimersEndocytosisExocytosisIntracellular Signaling Peptides and ProteinsMembrane ProteinsMiceMitosisNerve EndingsNeuropeptidesPhosphoproteinsPhosphorylationProtein BindingRatsVesicular Transport Proteins
1998
Calcium Dependence of Synaptic Vesicle Recycling Before and After Synaptogenesis
Coco S, Verderio C, De Camilli P, Matteoli M. Calcium Dependence of Synaptic Vesicle Recycling Before and After Synaptogenesis. Journal Of Neurochemistry 1998, 71: 1987-1992. PMID: 9798923, DOI: 10.1046/j.1471-4159.1998.71051987.x.Peer-Reviewed Original ResearchConceptsSynaptic contactsCalcium concentrationSynaptic vesicle exocytosisFormation of synapsesSynaptic vesicle recyclingVesicle recyclingHippocampal neuronsIntracellular calciumVesicle exocytosisImmunocytochemical assayNeurotransmitter releaseDifferent calcium concentrationsLow calcium concentrationsSynaptic vesiclesCalcium dependenceHigh calcium concentrationsSynaptogenesisNeuronsCalciumRelease componentCalcium sensorEndocytotic recyclingExocytosis
1997
Identification and characterization of homologues of the Exocyst component Sec10p
Guo W, Roth D, Gatti E, De Camilli P, Novick P. Identification and characterization of homologues of the Exocyst component Sec10p. FEBS Letters 1997, 404: 135-139. PMID: 9119050, DOI: 10.1016/s0014-5793(97)00109-9.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBrainCloning, MolecularCOS CellsExocytosisFungal ProteinsGolgi ApparatusHumansMammalsMolecular Sequence DataPolymerase Chain ReactionRecombinant ProteinsRNA, MessengerSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidTranscription, GeneticTransfectionVesicular Transport ProteinsConceptsC. elegans proteinsCharacterization of homologuesAmino acid identityBroad tissue distributionGolgi trafficMammalian counterpartsYeast SaccharomycesAcid identityGene productsCOS cellsWestern blot analysisSec10pPeripheral cytoplasmExocystBlot analysisProteinTissue distributionImmunofluorescence stainingSec8pCellsSaccharomycesCloningHomologuesExocytosisCytoplasmRecycling of Synaptic Vesicles
Bauerfeind R, David C, Grabs D, McPherson P, Nemoto Y, Slepnev V, Takei K, De Camilli P. Recycling of Synaptic Vesicles. Advances In Pharmacology 1997, 42: 253-257. PMID: 9327892, DOI: 10.1016/s1054-3589(08)60741-3.Peer-Reviewed Original ResearchConceptsSynaptic vesicle endocytosisSynaptic vesicle recyclingVesicle endocytosisVesicle recyclingSynaptic vesiclesPlasma membraneClathrin adaptor complex AP2Vesicular transport stepsClathrin-coated vesiclesClathrin-coated pitsMicrotubule-binding proteinSynaptic vesicle cycleRole of proteinsCell surface receptorsRole of lipidsClathrin coatRestrictive temperatureEarly endosomesGuanosine triphosphataseRecycling pathwayVesicle cycleDynaminTransport stepsEndocytosisSurface receptors
1996
Phosphoinositides as Regulators in Membrane Traffic
De Camilli P, Emr S, McPherson P, Novick P. Phosphoinositides as Regulators in Membrane Traffic. Science 1996, 271: 1533-1539. PMID: 8599109, DOI: 10.1126/science.271.5255.1533.Peer-Reviewed Original ResearchConceptsMembrane trafficActivation of proteinsGuanosine triphosphatasesSignal transductionVesicular transportRegulatory mechanismsPhosphatidylinositol metabolitesSecond messengerLocation signalPhosphorylated productsCell surfaceClassical rolePhosphoinositideCritical rolePhosphatidylinositolTransductionTriphosphatasesRegulatorProteinMessengerRegulationPolar headRoleRecruitmentActivationMolecular mechanisms in synaptic vesicle recycling
Bauerfeind R, Galli T, De Camilli P. Molecular mechanisms in synaptic vesicle recycling. Brain Cell Biology 1996, 25: 701-715. PMID: 9023719, DOI: 10.1007/bf02284836.Peer-Reviewed Original Research
1995
Molecular mechanisms in synaptic vesicle recycling
De Camilli P. Molecular mechanisms in synaptic vesicle recycling. FEBS Letters 1995, 369: 3-12. PMID: 7641879, DOI: 10.1016/0014-5793(95)00739-v.Peer-Reviewed Original ResearchConceptsSynaptic vesicle recyclingVesicle recyclingSynaptic vesiclesMolecular mechanismsPowerful experimental modelVesicular trafficVesicle reformationNon-peptide neurotransmittersSecretory organellesRecycling pathwayHigh specializationVesiclesSpecialized formExocytosisRecent studiesNerve terminalsOrganellesMechanismRecyclingAbundanceCurrent informationPathwayUnique propertiesKeeping synapses up to speed
De Camilli P. Keeping synapses up to speed. Nature 1995, 375: 450-451. PMID: 7777050, DOI: 10.1038/375450a0.Peer-Reviewed Original Research
1994
Formation of synaptic vesicles
Mundigl O, De Camilli P. Formation of synaptic vesicles. Current Opinion In Cell Biology 1994, 6: 561-567. PMID: 7986534, DOI: 10.1016/0955-0674(94)90077-9.Peer-Reviewed Original ResearchConceptsSynaptic vesiclesNew synaptic vesiclesSV membranesTransport vesiclesEvolutionary originNon-peptide neurotransmittersMembrane proteinsSecretory organellesFocal signalingVesiclesExocytosisMolecular compositionCellsGamma-aminobutyric acidOrganellesSignalingNerve cellsProteinDepolarization-induced Ca2PathwaySignificant progressNeurotransmittersMembraneAssemblyNerve terminalsThe role of Rab3A in neurotransmitter release
Geppert M, Bolshakov V, Siegelbaum S, Takei K, De Camilli P, Hammer R, Südhof T. The role of Rab3A in neurotransmitter release. Nature 1994, 369: 493-497. PMID: 7911226, DOI: 10.1038/369493a0.Peer-Reviewed Original ResearchConceptsRole of Rab3ASynaptic vesicle exocytosisSmall GTPRab3A geneHomologous recombinationVesicle exocytosisSynaptic vesiclesHippocampal CA1 pyramidal cellsSynaptic proteinsProteinCA1 pyramidal cellsNeurotransmitter releaseExocytosisGTPPyramidal cellsRepetitive stimulationSynaptic depressionElectrophysiological recordingsRepetitive stimuliCompensatory changesShort trainsRabphilinRab3Rab3AGenes
1991
Co-secretion of multiple signal molecules from endocrine cells via distinct exocytotic pathways
De Camilli P. Co-secretion of multiple signal molecules from endocrine cells via distinct exocytotic pathways. Trends In Pharmacological Sciences 1991, 12: 446-448. PMID: 1686507, DOI: 10.1016/0165-6147(91)90631-2.Peer-Reviewed Original Research
1978
Membrane events during stimulated exocytosis in exocrine glands [proceedings].
De Camilli P, Meldolesi J, Nassivera N, Tanaka Y. Membrane events during stimulated exocytosis in exocrine glands [proceedings]. The Journal Of Physiology 1978, 285: 25p-26p. PMID: 745077.Peer-Reviewed Original Research
1974
Structural Difference between Luminal and Lateral Plasmalemma in Pancreatic Acinar Cells
DE CAMILLI P, PELUCHETTI D, MELDOLESI J. Structural Difference between Luminal and Lateral Plasmalemma in Pancreatic Acinar Cells. Nature 1974, 248: 245-247. PMID: 4819419, DOI: 10.1038/248245b0.Peer-Reviewed Original Research