2024
Balancing acts: The posttranslational modification tightrope of flavivirus replication
Boytz R, Laurent-Rolle M. Balancing acts: The posttranslational modification tightrope of flavivirus replication. PLOS Pathogens 2024, 20: e1012626. PMID: 39466723, PMCID: PMC11516179, DOI: 10.1371/journal.ppat.1012626.Peer-Reviewed Original ResearchComprehensive Assessment of Inactivation Methods for Madariaga Virus
Boytz R, Keita K, Pawlak J, Laurent-Rolle M. Comprehensive Assessment of Inactivation Methods for Madariaga Virus. Viruses 2024, 16: 206. PMID: 38399982, PMCID: PMC10892135, DOI: 10.3390/v16020206.Peer-Reviewed Original ResearchConceptsEastern equine encephalitis virusMadariaga virusMethod of inactivationInactivation methodSelective agentNorth American strainsEEEV strainsEquine encephalitis virusTRIzol LSAmino acid levelsAmerican strainsEncephalitis virusAcid levelsTRIzolInfected cellsVirusFormalinInactivationPathogensPublic health threatInfected supernatantStrain
2023
CMPK2 restricts Zika virus replication by inhibiting viral translation
Pawlak J, Hsu J, Xia H, Han P, Suh H, Grove T, Morrison J, Shi P, Cresswell P, Laurent-Rolle M. CMPK2 restricts Zika virus replication by inhibiting viral translation. PLOS Pathogens 2023, 19: e1011286. PMID: 37075076, PMCID: PMC10150978, DOI: 10.1371/journal.ppat.1011286.Peer-Reviewed Original ResearchConceptsCytidine/uridine monophosphate kinase 2I interferon-stimulated genesZika virus replicationYellow fever virusAntiviral activityAntiviral effectVirus replicationKunjin virusType I interferon-stimulated genesFirst lineOverall antiviral responseHost's first lineEffective therapeutic interventionsViral translationBroad antiviral activityInterferon-stimulated genesGlobal health threatAntiviral treatmentFlaviviral infectionsPathogenic flavivirusesAntiviral functionDrug AdministrationTherapeutic interventionsAntiviral responseDengue virus
2022
Protocol for assessing translational regulation in mammalian cell lines by OP-Puro labeling
Hsu JC, Pawlak JB, Laurent-Rolle M, Cresswell P. Protocol for assessing translational regulation in mammalian cell lines by OP-Puro labeling. STAR Protocols 2022, 3: 101654. PMID: 36072758, PMCID: PMC9442383, DOI: 10.1016/j.xpro.2022.101654.Peer-Reviewed Original ResearchConceptsTranslational regulationMammalian cell linesDifferent cell typesDifferent growth conditionsMammalian cellsTranslation activityGene expressionBiological processesCell typesComplete detailsConfocal microscopyCell linesGrowth conditionsPuromycin analoguesCritical roleRegulationCellsProteinLabelingFundamental stepExpressionClick chemistry reactionActivityCytometry
2021
Translational shutdown and evasion of the innate immune response by SARS-CoV-2 NSP14 protein
Hsu JC, Laurent-Rolle M, Pawlak JB, Wilen CB, Cresswell P. Translational shutdown and evasion of the innate immune response by SARS-CoV-2 NSP14 protein. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2101161118. PMID: 34045361, PMCID: PMC8214666, DOI: 10.1073/pnas.2101161118.Peer-Reviewed Original ResearchConceptsSARS-CoV-2Interferon-stimulated genesImmune responseSevere acute respiratory syndrome coronavirus 2Acute respiratory syndrome coronavirus 2Host protein synthesisRespiratory syndrome coronavirus 2Syndrome coronavirus 2Innate immune responseUnprecedented global health crisisCoronavirus 2N7-methyltransferase activityOngoing COVID-19 pandemicHuman coronavirusesTranslational shutdownVirus replicationNsp14 proteinGlobal health crisisProtein synthesisInhibition activityCausative agentCOVID-19COVID-19 pandemicSARS-CoV-2 nsp14Dependent induction
2019
The Role of NS5 Protein in Determination of Host Cell Range for Yellow Fever Virus
Laurent-Rolle M, Morrison J. The Role of NS5 Protein in Determination of Host Cell Range for Yellow Fever Virus. DNA And Cell Biology 2019, 38: 1414-1417. PMID: 31633391, DOI: 10.1089/dna.2019.5115.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsHost-Specific NS5 Ubiquitination Determines Yellow Fever Virus Tropism
Miorin L, Laurent-Rolle M, Pisanelli G, Co PH, Albrecht RA, García-Sastre A, Morrison J. Host-Specific NS5 Ubiquitination Determines Yellow Fever Virus Tropism. Journal Of Virology 2019, 93: 10.1128/jvi.00151-19. PMID: 31043530, PMCID: PMC6600188, DOI: 10.1128/jvi.00151-19.Peer-Reviewed Original Research
2015
La Piedad Michoacán Mexico Virus V protein antagonizes type I interferon response by binding STAT2 protein and preventing STATs nuclear translocation
Pisanelli G, Laurent-Rolle M, Manicassamy B, Belicha-Villanueva A, Morrison J, Lozano-Dubernard B, Castro-Peralta F, Iovane G, García-Sastre. A. La Piedad Michoacán Mexico Virus V protein antagonizes type I interferon response by binding STAT2 protein and preventing STATs nuclear translocation. Virus Research 2015, 213: 11-22. PMID: 26546155, PMCID: PMC5538256, DOI: 10.1016/j.virusres.2015.10.027.Peer-Reviewed Original ResearchConceptsNuclear translocationParamyxoviridae familyIFN α/βSTAT2 proteinRubulavirus genusMost paramyxovirusesV proteinType I interferon responseI interferon responseCellular responsesSTAT2Innate immune responseAmino acidsType I interferonSignificant disease burdenProteinBlue eye diseaseMexico virusInterferon responseProtein levelsSwine cellsIFN responseSTAT1I interferonRespiratory distress
2014
The Interferon Signaling Antagonist Function of Yellow Fever Virus NS5 Protein Is Activated by Type I Interferon
Laurent-Rolle M, Morrison J, Rajsbaum R, Macleod JML, Pisanelli G, Pham A, Ayllon J, Miorin L, Martínez-Romero C, tenOever BR, García-Sastre A. The Interferon Signaling Antagonist Function of Yellow Fever Virus NS5 Protein Is Activated by Type I Interferon. Cell Host & Microbe 2014, 16: 314-327. PMID: 25211074, PMCID: PMC4176702, DOI: 10.1016/j.chom.2014.07.015.Peer-Reviewed Original ResearchUnanchored K48-Linked Polyubiquitin Synthesized by the E3-Ubiquitin Ligase TRIM6 Stimulates the Interferon-IKKε Kinase-Mediated Antiviral Response
Rajsbaum R, Versteeg GA, Schmid S, Maestre AM, Belicha-Villanueva A, Martínez-Romero C, Patel JR, Morrison J, Pisanelli G, Miorin L, Laurent-Rolle M, Moulton HM, Stein DA, Fernandez-Sesma A, tenOever BR, García-Sastre A. Unanchored K48-Linked Polyubiquitin Synthesized by the E3-Ubiquitin Ligase TRIM6 Stimulates the Interferon-IKKε Kinase-Mediated Antiviral Response. Immunity 2014, 40: 880-895. PMID: 24882218, PMCID: PMC4114019, DOI: 10.1016/j.immuni.2014.04.018.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntiviral AgentsCells, CulturedEnzyme ActivationHumansI-kappa B KinaseInterferon Type IJanus Kinase 1MicePhosphorylationPolyubiquitinRNA InterferenceRNA, Small InterferingSignal TransductionSTAT1 Transcription FactorTripartite Motif ProteinsUbiquitin-Conjugating EnzymesUbiquitin-Protein LigasesConceptsUnanchored K48Polyubiquitin chainsUnanchored polyubiquitin chainsUpregulation of hundredsAntiviral responseIκB kinase epsilonTripartite motif (TRIM) familyIKKε activationMotif familyKinase activationCellular factorsUBE2KReceptor signalingSTAT1 phosphorylationK48Kinase epsilonType I interferonIFN signalingIFN receptor signalingTRIM6Microbial infectionsISG inductionI interferonSignalingIKKε
2013
Dengue Virus Co-opts UBR4 to Degrade STAT2 and Antagonize Type I Interferon Signaling
Morrison J, Laurent-Rolle M, Maestre AM, Rajsbaum R, Pisanelli G, Simon V, Mulder LC, Fernandez-Sesma A, García-Sastre A. Dengue Virus Co-opts UBR4 to Degrade STAT2 and Antagonize Type I Interferon Signaling. PLOS Pathogens 2013, 9: e1003265. PMID: 23555265, PMCID: PMC3610674, DOI: 10.1371/journal.ppat.1003265.Peer-Reviewed Original ResearchConceptsDengue hemorrhagic feverType I interferonDengue feverSTAT2 degradationImmune evasionI interferonDENV NS5Anti-DENV therapeuticsDengue virus infectionDENV NS5 proteinVirus infectionHemorrhagic feverDENV replicationViral replicationAntiviral therapeuticsEfficient viral replicationIFNPotential severityFeverDENVInterferonNS5Competent cellsHost proteinsNS5 protein
2010
The NS5 Protein of the Virulent West Nile Virus NY99 Strain Is a Potent Antagonist of Type I Interferon-Mediated JAK-STAT Signaling
Laurent-Rolle M, Boer EF, Lubick KJ, Wolfinbarger JB, Carmody AB, Rockx B, Liu W, Ashour J, Shupert WL, Holbrook MR, Barrett AD, Mason PW, Bloom ME, García-Sastre A, Khromykh AA, Best SM. The NS5 Protein of the Virulent West Nile Virus NY99 Strain Is a Potent Antagonist of Type I Interferon-Mediated JAK-STAT Signaling. Journal Of Virology 2010, 84: 3503-3515. PMID: 20106931, PMCID: PMC2838099, DOI: 10.1128/jvi.01161-09.Peer-Reviewed Original ResearchConceptsPY-STAT1IFN antagonismJAK-STAT signal transductionIFN antagonistAlpha/beta interferonSignal transductionWild-type virusAnalogous residuesIFN-dependent gene expressionGene expressionJAK-STATKunjin virusSingle residueHost innate responseNonstructural protein NS5NS5 proteinSTAT1 phosphorylationSTAT2 degradationPoor suppressorNY99 strainPresence of IFNMutationsIFN-alpha/NS5Beta interferon
2009
NS5 of Dengue Virus Mediates STAT2 Binding and Degradation
Ashour J, Laurent-Rolle M, Shi PY, García-Sastre A. NS5 of Dengue Virus Mediates STAT2 Binding and Degradation. Journal Of Virology 2009, 83: 5408-5418. PMID: 19279106, PMCID: PMC2681973, DOI: 10.1128/jvi.02188-08.Peer-Reviewed Original ResearchConceptsDengue virusInnate immune responseInnate antiviral responseImmune responseInfection resultsIFN responseAntiviral responseDENV proteinsLevel of expressionAntiviral therapeuticsDENV genomeDecreased levelsInterferonPotential targetProtein levelsViral pathogensHost proteasesReduced levelsProteasome activityMultiple mechanismsVirusFacilitate infectionNovel mechanismViral polypeptidesNS5
2005
Inhibition of Alpha/Beta Interferon Signaling by the NS4B Protein of Flaviviruses
Muñoz-Jordán JL, Laurent-Rolle M, Ashour J, Martínez-Sobrido L, Ashok M, Lipkin WI, García-Sastre A. Inhibition of Alpha/Beta Interferon Signaling by the NS4B Protein of Flaviviruses. Journal Of Virology 2005, 79: 8004-8013. PMID: 15956546, PMCID: PMC1143737, DOI: 10.1128/jvi.79.13.8004-8013.2005.Peer-Reviewed Original ResearchConceptsSignal peptideCleavable signal peptidePositive-strand RNA virusesInterferon-stimulated response element promoterISRE promoter activationNonstructural protein NS4BB fusion proteinFunction of NS4BResponse element promoterActivation of STAT1Dengue virus NS4BNS2B/NS3Alpha/beta interferonAnti-interferon functionDeletion analysisIFN antagonismN-terminusPromoter activationFusion proteinStructural proteinsNonstructural proteinsInhibition of interferonRNA virusesViral polyprotein processingNS4B protein
2003
Inhibition of interferon signaling by dengue virus
Muñoz-Jordán J, Sánchez-Burgos GG, Laurent-Rolle M, García-Sastre A. Inhibition of interferon signaling by dengue virus. Proceedings Of The National Academy Of Sciences Of The United States Of America 2003, 100: 14333-14338. PMID: 14612562, PMCID: PMC283592, DOI: 10.1073/pnas.2335168100.Peer-Reviewed Original ResearchConceptsPositive-strand RNA genomeExpression of NS4BPositive-strand RNA virusesNuclear signal transducerVirus-encoded proteinsStrand RNA virusesStrand RNA genomeHuman A549 cellsDengue virusRNA genomeDengue virus-infected cellsSignal transducerTranscription 1Gene expressionUnknown functionVirus-infected cellsInhibition of interferonRNA virusesDengue virus infectionNS4BMosquito-borne flavivirusProteinA549 cellsIFN responseViral products