2012
Brassinosteroids regulate organ boundary formation in the shoot apical meristem of Arabidopsis
Gendron JM, Liu JS, Fan M, Bai MY, Wenkel S, Springer PS, Barton MK, Wang ZY. Brassinosteroids regulate organ boundary formation in the shoot apical meristem of Arabidopsis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 21152-21157. PMID: 23213257, PMCID: PMC3529081, DOI: 10.1073/pnas.1210799110.Peer-Reviewed Original ResearchConceptsShoot apical meristemOrgan boundary formationIdentity genesOrgan primordiaApical meristemGene expressionTranscription factor BZR1Architecture of plantsBR-insensitive mutantsBoundary formationSpatiotemporal controlOrgan boundariesStem cell nicheWT plantsUnderlying signaling mechanismsFusion phenotypeBrassinosteroidsCell nicheSignaling mechanismMeristemCentral meristemBZR1MutantsPrimordiaGenes
2011
PP2A activates brassinosteroid-responsive gene expression and plant growth by dephosphorylating BZR1
Tang W, Yuan M, Wang R, Yang Y, Wang C, Oses-Prieto JA, Kim TW, Zhou HW, Deng Z, Gampala SS, Gendron JM, Jonassen EM, Lillo C, DeLong A, Burlingame AL, Sun Y, Wang ZY. PP2A activates brassinosteroid-responsive gene expression and plant growth by dephosphorylating BZR1. Nature Cell Biology 2011, 13: 124-131. PMID: 21258370, PMCID: PMC3077550, DOI: 10.1038/ncb2151.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceArabidopsisArabidopsis ProteinsDNA-Binding ProteinsGene Expression Regulation, PlantMolecular Sequence DataNuclear ProteinsPhosphorylationPlant Growth RegulatorsPlants, Genetically ModifiedProtein Phosphatase 2Sequence AlignmentSignal TransductionTriazolesTwo-Hybrid System Techniques
2007
An Essential Role for 14-3-3 Proteins in Brassinosteroid Signal Transduction in Arabidopsis
Gampala SS, Kim TW, He JX, Tang W, Deng Z, Bai MY, Guan S, Lalonde S, Sun Y, Gendron JM, Chen H, Shibagaki N, Ferl RJ, Ehrhardt D, Chong K, Burlingame AL, Wang ZY. An Essential Role for 14-3-3 Proteins in Brassinosteroid Signal Transduction in Arabidopsis. Developmental Cell 2007, 13: 177-189. PMID: 17681130, PMCID: PMC2000337, DOI: 10.1016/j.devcel.2007.06.009.Peer-Reviewed Original ResearchMeSH Keywords14-3-3 ProteinsAmino Acid SequenceArabidopsisArabidopsis ProteinsBinding SitesCell NucleusDNA, PlantDNA-Binding ProteinsDown-RegulationModels, BiologicalMolecular Sequence DataMutationNuclear ProteinsPhosphorylationPlant LeavesProtein BindingProtein KinasesProtein TransportSignal TransductionSteroids, HeterocyclicConceptsPlant growthTranscription factorsNuclear localizationGene expressionBZR2/BES1Brassinosteroid signal transductionSignal transduction pathwaysKey transcription factorRapid nuclear localizationBZR1 proteinCell surface receptorsBR regulationBR deficiencyTransgenic plantsGSK3 kinaseBR responsesCytoplasmic retentionSignal transductionTransduction pathwaysCytoplasmic localizationDNA bindingBrassinosteroidsBR treatmentBZR1Essential hormone
2005
BZR1 Is a Transcriptional Repressor with Dual Roles in Brassinosteroid Homeostasis and Growth Responses
He JX, Gendron JM, Sun Y, Gampala SS, Gendron N, Sun CQ, Wang ZY. BZR1 Is a Transcriptional Repressor with Dual Roles in Brassinosteroid Homeostasis and Growth Responses. Science 2005, 307: 1634-1638. PMID: 15681342, PMCID: PMC2925132, DOI: 10.1126/science.1107580.Peer-Reviewed Original ResearchMeSH KeywordsArabidopsisArabidopsis ProteinsBase SequenceBinding SitesChromatin ImmunoprecipitationDNA-Binding ProteinsFeedback, PhysiologicalGene Expression Regulation, PlantGenes, PlantGenes, ReporterHomeostasisLightMutationNuclear ProteinsOligonucleotide Array Sequence AnalysisPhenotypePlant Growth RegulatorsPlants, Genetically ModifiedPromoter Regions, GeneticRecombinant Fusion ProteinsRepressor ProteinsSignal TransductionSteroidsTranscription, GeneticConceptsBrassinosteroid homeostasisTranscriptional repressorCell surface receptor kinaseBR biosynthetic genesDevelopment of plantsGrowth responseBR homeostasisBR biosynthesisBiosynthetic genesBZR1Dual roleReceptor kinaseAdditional potential targetsGene expressionMicroarray analysisUnknown DNARepressorNormal growthHomeostasisPotential targetPhysiological studiesDephosphorylationBiosynthesisKinaseGenes
2002
The GSK3-like kinase BIN2 phosphorylates and destabilizes BZR1, a positive regulator of the brassinosteroid signaling pathway in Arabidopsis
He JX, Gendron JM, Yang Y, Li J, Wang ZY. The GSK3-like kinase BIN2 phosphorylates and destabilizes BZR1, a positive regulator of the brassinosteroid signaling pathway in Arabidopsis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2002, 99: 10185-10190. PMID: 12114546, PMCID: PMC126645, DOI: 10.1073/pnas.152342599.Peer-Reviewed Original ResearchMeSH KeywordsArabidopsisArabidopsis ProteinsCalcium-Calmodulin-Dependent Protein KinasesDNA-Binding ProteinsGene Expression Regulation, PlantGlycogen Synthase Kinase 3Glycogen Synthase KinasesGreen Fluorescent ProteinsLuminescent ProteinsNuclear ProteinsPhosphorylationPhytosterolsPlants, Genetically ModifiedProtein KinasesSignal TransductionConceptsPositive regulatorBZR2/BES1Two-hybrid assayBZR1 proteinReceptor BRI1BZR1Proteasome machineryBrassinosteroidsBIN2 activityNegative regulatorBIN2Protein accumulationRegulatorNormal growthDephosphorylationProteasome inhibitorsAccumulationBES1BRI1ArabidopsisPhosphorylatesSteroid hormonesMG132YeastPhosphorylationNuclear-Localized BZR1 Mediates Brassinosteroid-Induced Growth and Feedback Suppression of Brassinosteroid Biosynthesis
Wang Z, Nakano T, Gendron J, He J, Chen M, Vafeados D, Yang Y, Fujioka S, Yoshida S, Asami T, Chory J. Nuclear-Localized BZR1 Mediates Brassinosteroid-Induced Growth and Feedback Suppression of Brassinosteroid Biosynthesis. Developmental Cell 2002, 2: 505-513. PMID: 11970900, DOI: 10.1016/s1534-5807(02)00153-3.Peer-Reviewed Original ResearchConceptsBR biosynthesisBR signal transduction pathwayCell surface receptor kinasePlant steroid hormonesSignal transduction pathwaysBZR1 proteinBrassinosteroid biosynthesisBR responsesPositive regulatorReceptor kinaseTransduction pathwaysBrassinosteroidsBZR1Gene expressionDominant mutationsFeedback regulationBiosynthesisNuclear componentsMutationsRegulationPathwayBRI1Steroid hormonesKinaseHypocotyls