2024
A circadian clock output functions independently of phyB to sustain daytime PIF3 degradation
Liu W, Lowrey H, Xu A, Leung C, Adamchek C, He J, Du J, Chen M, Gendron J. A circadian clock output functions independently of phyB to sustain daytime PIF3 degradation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2024, 121: e2408322121. PMID: 39163340, PMCID: PMC11363348, DOI: 10.1073/pnas.2408322121.Peer-Reviewed Original ResearchConceptsPhytochrome-interacting factorsF-boxRed-light photoreceptor phytochrome BPhotoreceptor phytochrome BCircadian clock outputE3 ubiquitin ligaseDownstream biological processesPhyB signalingGrowth-regulating mechanismsTranscriptional connectionsPhytochrome BClock's roleRhythmic transcriptionUbiquitin ligaseClock regulationGenetic evidenceMonochromatic red lightProtein stabilityCircadian clockPhyBBiological processesPlant growthClock outputCircadian expressionRegulation mechanismPlants distinguish different photoperiods to independently control seasonal flowering and growth
Wang Q, Liu W, Leung C, Tarté D, Gendron J. Plants distinguish different photoperiods to independently control seasonal flowering and growth. Science 2024, 383: eadg9196. PMID: 38330117, PMCID: PMC11134419, DOI: 10.1126/science.adg9196.Peer-Reviewed Original ResearchConceptsPhotoperiodic growthSeasonal floweringControl expression of genesDuration of photosynthetic activityGrowth-regulating pathwaysExpression of genesPhotosynthetic periodMeasure photoperiodPhotoperiodic floweringPhotosynthetic activityControl expressionFlowering mechanismRegulation pathwaysSeasonal growthFlowersLong daysDifferent photoperiodsPhotoperiodLight intensityPlantsGrowthMutantsGenesPathway
2023
Phosphoproteome analyses pinpoint the F‐box protein SLOW MOTION as a regulator of warm temperature‐mediated hypocotyl growth in Arabidopsis
Zhu S, Pan L, Vu L, Xu X, Orosa‐Puente B, Zhu T, Neyt P, van de Cotte B, Jacobs T, Gendron J, Spoel S, Gevaert K, De Smet I. Phosphoproteome analyses pinpoint the F‐box protein SLOW MOTION as a regulator of warm temperature‐mediated hypocotyl growth in Arabidopsis. New Phytologist 2023, 241: 687-702. PMID: 37950543, PMCID: PMC11091872, DOI: 10.1111/nph.19383.Peer-Reviewed Original ResearchHEXOKINASE1 and glucose-6-phosphate fuel plant growth and development
Vanderwall M, Gendron J. HEXOKINASE1 and glucose-6-phosphate fuel plant growth and development. Development 2023, 150 PMID: 37842778, PMCID: PMC10617624, DOI: 10.1242/dev.202346.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsCatalytic functionPlant metabolic pathwaysEnzyme catalytic functionPlant developmentPhotoautotrophic organismsGlucose-6-phosphate productionHexokinase1Plant growthPhosphorylation of hexosesBiosynthetic routeMetabolic enzymesMetabolic pathwaysPlantsDevelopmental significancePathwayPhosphorylationOrganismsEnzymeMetabolismPigmentsPrevious studiesHexosesDiscrete areasCatalytic activityFunctionIntegrating the dynamic and energetic fields of metabolism and development
Finley L, Gendron J, Miguel-Aliaga I, Rutter J. Integrating the dynamic and energetic fields of metabolism and development. Development 2023, 150 PMID: 37883064, PMCID: PMC10765412, DOI: 10.1242/dev.202424.Commentaries, Editorials and LettersSystematic characterization of photoperiodic gene expression patterns reveals diverse seasonal transcriptional systems in Arabidopsis
Leung C, Tarté D, Oliver L, Wang Q, Gendron J. Systematic characterization of photoperiodic gene expression patterns reveals diverse seasonal transcriptional systems in Arabidopsis. PLOS Biology 2023, 21: e3002283. PMID: 37699055, PMCID: PMC10497145, DOI: 10.1371/journal.pbio.3002283.Peer-Reviewed Original ResearchConceptsExpression patternsCis-element analysisPhenylpropanoid biosynthesis pathwaySeasonal expression patternsImportant cellular pathwaysGene expression patternsThousands of genesFunctional enrichment analysisGene expression dataAlign growthArabidopsis plantsPhotoperiodic floweringTranscriptional networksPhotoperiodic genesBiosynthesis pathwayCellular processesPhenylpropanoid pathwayTranscriptomic experimentsTranscriptomic dataTranscriptional systemCellular pathwaysEnrichment analysisGene expressionGene clusteringExpression dataArabidopsis Tubby domain‐containing F‐box proteins positively regulate immunity by modulating PI4Kβ protein levels
Devendrakumar K, Copeland C, Adamchek C, Zhong X, Huang X, Gendron J, Li X. Arabidopsis Tubby domain‐containing F‐box proteins positively regulate immunity by modulating PI4Kβ protein levels. New Phytologist 2023, 240: 354-371. PMID: 37571862, PMCID: PMC11114105, DOI: 10.1111/nph.19187.Peer-Reviewed Original ResearchConceptsTubby-like proteinsN-terminal F-box domainF-box E3 ligase complexesImmunoprecipitation-mass spectrometry analysisArabidopsis Immune ResponsesPlant immune responsesProtein levelsDomain-containing proteinsF-box domainF-box proteinsE3 ligase complexProteasome-dependent mannerRedundant homologsOverexpression linesTubby domainUbiquitination substratesSkp1-CullinTLP functionsLigase complexTubby proteinBiosynthesis enzymesDouble mutantMass spectrometry analysisProtein 6Novel mechanismNew Horizons in Plant Photoperiodism
Gendron J, Staiger D. New Horizons in Plant Photoperiodism. Annual Review Of Plant Biology 2023, 74: 481-509. PMID: 36854481, PMCID: PMC11114106, DOI: 10.1146/annurev-arplant-070522-055628.Peer-Reviewed Original ResearchConceptsPlant photoperiodismRecent molecular genetic studiesGene regulatory networksMolecular genetic studiesPhotoperiodic floweringPlant speciesRegulatory networksDevelopmental processesMetabolic networksGenetic studiesPhotoperiodismSeasonal changesOrganismsPlantsPotential rolePhotoperiodClimate changeLatitudinal migrationTranscriptomicsFloweringSpeciesGrowthMigrationReproductionRice
2022
Sugar sensation and mechanosensation in the egg-laying preference shift of Drosophila suzukii
Wang W, Dweck H, Talross G, Zaidi A, Gendron J, Carlson J. Sugar sensation and mechanosensation in the egg-laying preference shift of Drosophila suzukii. ELife 2022, 11: e81703. PMID: 36398882, PMCID: PMC9674340, DOI: 10.7554/elife.81703.Peer-Reviewed Original ResearchConceptsEgg-laying preferenceTaste organsAgricultural pestsDrosophila suzukiiChannel genesStiff substratesDrosophilaTaste sensillaReceptor geneMechanosensory cuesFruit sugarsGenesMechanosensationTaste sensationBitter taste sensationSpeciesEggsSweet taste sensationMajor subsetSuzukiiPestsSugarsElectrophysiological responsesWeak preferenceVariety of changesKARRIKIN UP-REGULATED F-BOX 1 (KUF1) imposes negative feedback regulation of karrikin and KAI2 ligand metabolism in Arabidopsis thaliana
Sepulveda C, Guzmán MA, Li Q, Villaécija-Aguilar JA, Martinez SE, Kamran M, Khosla A, Liu W, Gendron JM, Gutjahr C, Waters MT, Nelson DC. KARRIKIN UP-REGULATED F-BOX 1 (KUF1) imposes negative feedback regulation of karrikin and KAI2 ligand metabolism in Arabidopsis thaliana. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2112820119. PMID: 35254909, PMCID: PMC8931227, DOI: 10.1073/pnas.2112820119.Peer-Reviewed Original ResearchConceptsKAI2-ligandsF-BOX 1Fire-prone environmentsArabidopsis thalianaNegative feedback loopKarrikinsNegative feedback regulationFeedback regulationExpression increasesPlantsGerminationLigand metabolismFeedback loopFurther activationMetabolismThalianaBiosynthesisGenesSpeciesRegulationPathwayActivationDiscoveryResponseGrowth
2021
Unique N-Terminal Interactions Connect F-BOX STRESS INDUCED (FBS) Proteins to a WD40 Repeat-like Protein Pathway in Arabidopsis
Sepulveda-Garcia E, Fulton EC, Parlan EV, O’Connor L, Fleming AA, Replogle AJ, Rocha-Sosa M, Gendron JM, Thines B. Unique N-Terminal Interactions Connect F-BOX STRESS INDUCED (FBS) Proteins to a WD40 Repeat-like Protein Pathway in Arabidopsis. Plants 2021, 10: 2228. PMID: 34686037, PMCID: PMC8537223, DOI: 10.3390/plants10102228.Peer-Reviewed Original ResearchFBX proteinsSCF complexSCF-type E3 ubiquitin ligasesProtein targetsF-box domainF-box proteinsProtein degradation eventsE3 ubiquitin ligasesFBS proteinsC-terminal domainSkp proteinInteracting proteinSubstrate adaptorUbiquitin ligasesDomain interactsEnvironmental stressBiological functionsC-terminusN-terminusFacilitate recruitmentProtein pathwayFB proteinNuclear eventsArabidopsisProteinEnergy as a seasonal signal for growth and reproduction
Gendron JM, Leung CC, Liu W. Energy as a seasonal signal for growth and reproduction. Current Opinion In Plant Biology 2021, 63: 102092. PMID: 34461431, DOI: 10.1016/j.pbi.2021.102092.Peer-Reviewed Original ResearchA metabolic daylength measurement system mediates winter photoperiodism in plants
Liu W, Feke A, Leung CC, Tarté DA, Yuan W, Vanderwall M, Sager G, Wu X, Schear A, Clark DA, Thines BC, Gendron JM. A metabolic daylength measurement system mediates winter photoperiodism in plants. Developmental Cell 2021, 56: 2501-2515.e5. PMID: 34407427, PMCID: PMC8440495, DOI: 10.1016/j.devcel.2021.07.016.Peer-Reviewed Original ResearchConceptsPhotoperiodic floweringPlant fitnessCellular healthMetabolic networksStudy systemPhotoperiodic regulationWinter-like photoperiodsVegetative healthPhotoperiodic expressionsSucrose levelsPlantsStarch productionFloweringPhotoperiodismArabidopsisPhotosynthesisGenesPromoterFlowersBioinformaticsReporterLuciferaseFitnessRegulationPhotoperiodA microProtein repressor complex in the shoot meristem controls the transition to flowering
Rodrigues VL, Dolde U, Sun B, Blaakmeer A, Straub D, Eguen T, Botterweg-Paredes E, Hong S, Graeff M, Li MW, Gendron JM, Wenkel S. A microProtein repressor complex in the shoot meristem controls the transition to flowering. Plant Physiology 2021, 187: 187-202. PMID: 34015131, PMCID: PMC8418433, DOI: 10.1093/plphys/kiab235.Peer-Reviewed Original ResearchConceptsShoot apical meristemEarly flowering phenotypeGenetic suppressor screenPost-translational regulatorFloral repressionSuppressor screenChromatin modificationsFloral transitionFloral meristemRepressive complexesShoot meristemRepressor complexGenetic interactionsApical meristemCONSTANSEarly floweringShoot apexUndifferentiated stateJMJ14Sequencing studiesMeristemAdditional roleSum1MicroproteinsPlantsFunctional domain studies uncover novel roles for the ZTL Kelch repeat domain in clock function
Feke A, Vanderwall M, Liu W, Gendron JM. Functional domain studies uncover novel roles for the ZTL Kelch repeat domain in clock function. PLOS ONE 2021, 16: e0235938. PMID: 33730063, PMCID: PMC7968664, DOI: 10.1371/journal.pone.0235938.Peer-Reviewed Original ResearchConceptsLOV KELCH PROTEIN 2Kelch repeat domainRepeat domainLOV domainsF-BOX PROTEIN 1Protein-protein interaction domainsPlant circadian clockFLAVIN-BINDING KELCHProtein domain architecturePotential interacting partnerE3 ubiquitin ligasesInteracting partnerDomain architectureProtein complexesUbiquitin ligasesKelch proteinProtein familyTransgenic linesFlowering timeKelch familyProtein domainsZEITLUPEInteraction domainClock functionUnique photoreceptor
2020
A Decoy Library Uncovers U-Box E3 Ubiquitin Ligases That Regulate Flowering Time in Arabidopsis
Feke AM, Hong J, Liu W, Gendron JM. A Decoy Library Uncovers U-Box E3 Ubiquitin Ligases That Regulate Flowering Time in Arabidopsis. Genetics 2020, 215: 699-712. PMID: 32434795, PMCID: PMC7337086, DOI: 10.1534/genetics.120.303199.Peer-Reviewed Original ResearchConceptsE3 ubiquitin ligasesUbiquitin ligasesU-box E3 ubiquitin ligasesReproductive developmentReverse genetic screenMOS4-associated complexDominant-negative strategyGenetic redundancyGenetic screenTransgenic populationsTargeted degradationLigasesUnidentified regulatorDevelopmental transitionsBiological processesGenetic characterizationU-boxBiochemical studiesWidespread importanceRegulatorArabidopsisOrganismsPlantsProteinComplexesSame Concept Different Outcomes: Sugars Determine Circadian Clock Protein Fate in Animals and Plants
Liu W, Gendron J. Same Concept Different Outcomes: Sugars Determine Circadian Clock Protein Fate in Animals and Plants. Molecular Plant 2020, 13: 360-362. PMID: 32092448, DOI: 10.1016/j.molp.2020.02.013.Peer-Reviewed Original Research
2019
GIGANTEA recruits the UBP12 and UBP13 deubiquitylases to regulate accumulation of the ZTL photoreceptor complex
Lee CM, Li MW, Feke A, Liu W, Saffer AM, Gendron JM. GIGANTEA recruits the UBP12 and UBP13 deubiquitylases to regulate accumulation of the ZTL photoreceptor complex. Nature Communications 2019, 10: 3750. PMID: 31434902, PMCID: PMC6704089, DOI: 10.1038/s41467-019-11769-7.Peer-Reviewed Original ResearchConceptsDeubiquitylating enzymesCircadian clockTarget proteinsE3 ubiquitin ligase activityPlant circadian clockUbiquitin-specific protease 12Post-transcriptional mechanismsUbiquitin ligase activityPhotoreceptor complexZTL proteinProtein ubiquitylationInteracting partnerProtein complexesLigase activityZEITLUPEUBP12Gi protein levelsUBP13Enzyme typeLight conditionsGiganteaProtein levelsProteinEnzymeDay light conditionsDecoys provide a scalable platform for the identification of plant E3 ubiquitin ligases that regulate circadian function
Feke A, Liu W, Hong J, Li MW, Lee CM, Zhou EK, Gendron JM. Decoys provide a scalable platform for the identification of plant E3 ubiquitin ligases that regulate circadian function. ELife 2019, 8: e44558. PMID: 30950791, PMCID: PMC6483598, DOI: 10.7554/elife.44558.Peer-Reviewed Original ResearchConceptsE3 ubiquitin ligasesUbiquitin ligasesCircadian clockCircadian functionPlant E3 ubiquitin ligasesTransgenic Arabidopsis plantsNew potential regulatorsArabidopsis plantsRegulated degradationPlant developmentClock proteinsClock regulatorsFunctional redundancyE3 ubiquitinProtein degradationGenetic challengesLigasesPotential regulatorCircadian periodScreening platformUbiquitinRegulatorDecoysSplicingClock
2018
Characterization of Two Growth Period QTLs Reveals Modification of PRR3 Genes During Soybean Domestication
Li MW, Liu W, Lam HM, Gendron JM. Characterization of Two Growth Period QTLs Reveals Modification of PRR3 Genes During Soybean Domestication. Plant And Cell Physiology 2018, 60: 407-420. PMID: 30418611, DOI: 10.1093/pcp/pcy215.Peer-Reviewed Original ResearchConceptsQuantitative trait lociTranscription repressor complexMultiple interval mappingCCT domainSoybean domesticationSoybean orthologsGrowth periodRepressor complexTrait lociGenomic regionsSoybean improvementGenetic networksSubcellular localizationInterval mappingTranscription factorsShort growth periodLociGp12GmFT2aGmFT5aE locusE2 locusGp11High expressionMutations