2023
Arabidopsis Tubby domain‐containing F‐box proteins positively regulate immunity by modulating PI4Kβ protein levels
Devendrakumar K, Copeland C, Adamchek C, Zhong X, Huang X, Gendron J, Li X. Arabidopsis Tubby domain‐containing F‐box proteins positively regulate immunity by modulating PI4Kβ protein levels. New Phytologist 2023, 240: 354-371. PMID: 37571862, PMCID: PMC11114105, DOI: 10.1111/nph.19187.Peer-Reviewed Original ResearchConceptsTubby-like proteinsN-terminal F-box domainF-box E3 ligase complexesImmunoprecipitation-mass spectrometry analysisArabidopsis Immune ResponsesPlant immune responsesProtein levelsDomain-containing proteinsF-box domainF-box proteinsE3 ligase complexProteasome-dependent mannerRedundant homologsOverexpression linesTubby domainUbiquitination substratesSkp1-CullinTLP functionsLigase complexTubby proteinBiosynthesis enzymesDouble mutantMass spectrometry analysisProtein 6Novel mechanism
2019
GIGANTEA recruits the UBP12 and UBP13 deubiquitylases to regulate accumulation of the ZTL photoreceptor complex
Lee CM, Li MW, Feke A, Liu W, Saffer AM, Gendron JM. GIGANTEA recruits the UBP12 and UBP13 deubiquitylases to regulate accumulation of the ZTL photoreceptor complex. Nature Communications 2019, 10: 3750. PMID: 31434902, PMCID: PMC6704089, DOI: 10.1038/s41467-019-11769-7.Peer-Reviewed Original ResearchConceptsDeubiquitylating enzymesCircadian clockTarget proteinsE3 ubiquitin ligase activityPlant circadian clockUbiquitin-specific protease 12Post-transcriptional mechanismsUbiquitin ligase activityPhotoreceptor complexZTL proteinProtein ubiquitylationInteracting partnerProtein complexesLigase activityZEITLUPEUBP12Gi protein levelsUBP13Enzyme typeLight conditionsGiganteaProtein levelsProteinEnzymeDay light conditions