2018
155 Loss of PRDX1 increases RAD51 Cys319 oxidation and decreases homologous recombination
Skoko J, Asan A, Woodcock C, Cao J, Gaboriau D, Paulsen C, Attar M, Wingert B, Woodcock S, Schulte J, Ma H, Camacho C, Liu Y, Morrison C, Carroll K, Freeman B, Neumann C. 155 Loss of PRDX1 increases RAD51 Cys319 oxidation and decreases homologous recombination. Free Radical Biology And Medicine 2018, 128: s73-s74. DOI: 10.1016/j.freeradbiomed.2018.10.159.Peer-Reviewed Original ResearchRAD51 foci formationDNA double-strand breaksHomologous recombinationPeroxiredoxin 1Foci formationSuppression of HRDNA damageSulfenic acid formDouble-strand breaksSite-directed mutagenesisSoft agar colony formationAgar colony formationMDA-MB-231 breast cancer cellsGenomic stabilityBind RAD51DNA repairImmunoprecipitation assaysCell signalingOxidation of cysteineRAD51Bind proteinsC319Enhanced DNA damageBreast cancer cellsProtein sensors
2017
138 Redox Regulation of RAD51 and Homologous Recombination by Peroxiredoxin 1 and Electrophilic Nitro-fatty Acids
Skoko J, Asan A, Woodcock C, Cao J, Gaboriau D, Paulsen C, Attar M, Wingert B, Woodcock S, Schulte J, Ma H, Camacho C, Liu Y, Morrison C, Carroll K, Freeman B, Neumann C. 138 Redox Regulation of RAD51 and Homologous Recombination by Peroxiredoxin 1 and Electrophilic Nitro-fatty Acids. Free Radical Biology And Medicine 2017, 112: 100-101. DOI: 10.1016/j.freeradbiomed.2017.10.151.Peer-Reviewed Original ResearchOA-NO2Breast cancer cellsPeroxiredoxin 1Sensitization of cellsElectrophilic nitro-fatty acidsCancer cellsHuman breast cancer cellsNitro-fatty acidsPharmacological treatmentBreast cancerDNA damageRAD51 foci formationFoci formationCancerDNA double-strand breaksHomologous recombination repairPrimary targetSensitizationRepairCellsCellular responsesH2AX phosphorylationRecombination repairHomologous recombinationRedox regulation