Yale Combined Program in the Biological and Biomedical Sciences (BBS): Biochemistry, Quantitative Biology, Biophysics and Structural Biology (BQBS)
Extensive Research Description
Our research group is interested in mechanistic studies of chemical reactions at the single molecule level. Single molecule force manipulation and optical detection have revolutionized biophysics by characterizing states and kinetics traditionally obscured in ensemble averages; the development of single molecule vibrational spectroscopies will provide the structural resolution necessary to extend these tools to study chemical processes such as bond formation and dissociation, charge transfer, and ligand exchange. We will apply an array of single molecule techniques to study how metalloproteins facilitate redox chemistry in their active sites and to study mechanistic heterogeneity during carbon-carbon bond formation in organometallic catalysts. New theoretical and computational methods will be developed to understand these new spectroscopies and model reaction pathways.
Insulin dimer dissociation and unfolding revealed by amide I two-dimensional infrared spectroscopy.
Ganim Z, Jones KC, Tokmakoff A. Insulin dimer dissociation and unfolding revealed by amide I two-dimensional infrared spectroscopy. Physical Chemistry Chemical Physics : PCCP 2010, 12:3579-88. 2010
Single-molecule vibrational spectroscopy adds structural resolution to the optical trap.
Ganim Z. Single-molecule vibrational spectroscopy adds structural resolution to the optical trap. Biophysical Journal 2013, 104:4-5. 2013
Amide I two-dimensional infrared spectroscopy of proteins.
Ganim Z, Chung HS, Smith AW, Deflores LP, Jones KC, Tokmakoff A. Amide I two-dimensional infrared spectroscopy of proteins. Accounts Of Chemical Research 2008, 41:432-41. 2008