Featured Publications
Targeted two-photon chemical apoptotic ablation of defined cell types in vivo
Hill RA, Damisah EC, Chen F, Kwan AC, Grutzendler J. Targeted two-photon chemical apoptotic ablation of defined cell types in vivo. Nature Communications 2017, 8: 15837. PMID: 28621306, PMCID: PMC5501159, DOI: 10.1038/ncomms15837.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBrainCells, CulturedFemaleGreen Fluorescent ProteinsLaser TherapyMaleMiceNeuronsPhotonsSpecies SpecificityConceptsCell deathNucleic acid-binding dyeVivo functional consequencesCell type differencesPattern of apoptosisDose-dependent apoptosisComplex organismsMitochondrial fissionFluorescent proteinUnderstanding of mechanismsCell typesCell clearanceFunctional consequencesIndividual cellsDiverse organsDistinct populationsApoptosisMouse brainZebrafishMajor bottleneckNeural plasticityOrganismsSpeciesProteinApoptotic
2021
Caveolae-mediated Tie2 signaling contributes to CCM pathogenesis in a brain endothelial cell-specific Pdcd10-deficient mouse model
Zhou HJ, Qin L, Jiang Q, Murray KN, Zhang H, Li B, Lin Q, Graham M, Liu X, Grutzendler J, Min W. Caveolae-mediated Tie2 signaling contributes to CCM pathogenesis in a brain endothelial cell-specific Pdcd10-deficient mouse model. Nature Communications 2021, 12: 504. PMID: 33495460, PMCID: PMC7835246, DOI: 10.1038/s41467-020-20774-0.Peer-Reviewed Original ResearchConceptsCerebral cavernous malformationsCCM lesionsSmooth muscle actin-positive pericytesEndothelial cell lossRegions of brainCCM pathogenesisPost-capillary venulesCerebral hemorrhagePharmacological blockadeVascular abnormalitiesEC-specific deletionCavernous malformationsMouse modelCell lossMicrovascular bedGenetic deletionLesion formationLesionsVascular dynamicsBarrier functionMicrovascular structureTwo-photon microscopyTie2PathogenesisMice
2009
Ballistic delivery of dyes for structural and functional studies of the nervous system.
Gan WB, Grutzendler J, Wong RO, Lichtman JW. Ballistic delivery of dyes for structural and functional studies of the nervous system. Cold Spring Harbor Protocols 2009, 2009: pdb.prot5202. PMID: 20147144, PMCID: PMC2916724, DOI: 10.1101/pdb.prot5202.Peer-Reviewed Original Research
2000
Multicolor “DiOlistic” Labeling of the Nervous System Using Lipophilic Dye Combinations
Gan W, Grutzendler J, Wong W, Wong R, Lichtman J. Multicolor “DiOlistic” Labeling of the Nervous System Using Lipophilic Dye Combinations. Neuron 2000, 27: 219-225. PMID: 10985343, DOI: 10.1016/s0896-6273(00)00031-3.Peer-Reviewed Original ResearchConceptsNervous systemGolgi-like labelingNormal synaptic responsesComplex neuronal networksDendritic remodelingSynaptic responsesNeuronal preparationsNeuronal connectivityNeuronal circuitsIndividual neuronsNeuronsDye-coated particlesGene gunNeuronal networksStructural plasticityLipophilic dyeCellsGliaDiOlisticsLabeling