Biochemistry; Biophysics; Cell Nucleus; Chromatin; Molecular Biology; Transcription Factors; Gene Expression
Gene expression is exquisitely regulated during cellular growth and differentiation. Importantly, how the nuclear structure and dynamics encode additional regulatory information is not well understood. Single cell analysis such as advanced cell imaging has the potential to bridge the significant gap between biochemistry and genetics and to enable the analysis of gene regulation within its native nuclear environment. Our laboratory combines cellular imaging and molecular methods to characterize nuclear structure and dynamics, and to explore its functional implications.
Specialized Terms: Transcription regulation; Chromatin; Nuclear periphery; Nuclear compartmentalization; Protein dynamics; Cellular biophysics and imaging
Extensive Research Description
- Wu, F. and Yao, J. (2013). Spatial Compartmentalization of the Nuclear Periphery Characterized by Genome-wide Mapping, BMC Genomics, 14: 591
- Yao, J., Fetter, R. D., Hu, P., Betzig, E., and Tjian, R. (2011). Subnuclear Segregation of Genes and Core Promoter Factors in Myogenesis, Genes & Development, 25: 569-580
- Wu, F., Olson B. G., and Yao, J. (2016) DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments, Journal of Visualized Experiments, 107:e53620
- Yao, J. (2016) Imaging Transcriptional Regulation of Eukaryotic mRNA Genes: Advances and Outlook. Journal of Molecular Biology, pii: S0022-2836(16) 30489-2. doi: 10.1016/j.jmb.2016.11.007.