Gene expression is exquisitely regulated during cellular growth and differentiation. Importantly, how the nuclear structure and dynamics encode additional regulatory information is not well understood. Single cell analysis such as advanced cell imaging has the potential to bridge the significant gap between biochemistry and genetics and to enable the analysis of gene regulation within its native nuclear environment. Our laboratory combines cellular imaging and molecular methods to characterize nuclear structure and dynamics, and to explore its functional implications.
Extensive Research Description
- Wu, F. and Yao, J. (2013). Spatial Compartmentalization of the Nuclear Periphery Characterized by Genome-wide Mapping, BMC Genomics, 14: 591
- Yao, J., Fetter, R. D., Hu, P., Betzig, E., and Tjian, R. (2011). Subnuclear Segregation of Genes and Core Promoter Factors in Myogenesis, Genes & Development, 25: 569-580
- Darzacq, X., Yao, J., Larson, D.R., Causse, C.Z., Bonasac, L., de Turris, V., Ruda, V.M., Lionnet, T., Zenklusen, D., Guglielmi, B., Tjian, R., and Singer, R.H. (2009). Imaging Transcription in Living Cells, Annual Review of Biophysics 38:173-196
- Yao, J., Zobeck, K.L., Lis, J.T. and Webb, W.W. (2008). Imaging Transcription Dynamics at Endogenous Genes in Living Drosophila Tissues, Methods, 45: 233-241
- Yao, J., Ardehali, M.B., Fecko, C. J., Webb, W.W., and Lis, J.T. (2007). Intranuclear Distribution and Local Dynamics of RNA Polymerase II During Transcription Activation, Molecular Cell, 28: 978-990
- Yao, J., Munson, K.M., Webb, W.W., and Lis, J.T. (2006). Dynamics of Heat Shock Factor Association with Native Gene Loci in Living Cells, Nature, 442: 1050-1053