Antibody Optimization and Titration
Expression range graph for protein X
Antibody titration is performed to optimize signal to noise ratio and achieve optimal dynamic range for a biomarker of interest. In this example a titer of 1 to 50 is chosen over 1 to 25 because of less background signal.
Western Blot of protein X and QIF of protein X on cell line TMA
After titer optimization the antibody is further validated for reproducibility and specificity (Western Blot)