Services

Cytokine Detection and Quantification

Cytokines are important cell signaling proteins, mediating a wide range of physiological responses, including immunity, inflammation, and hematopoiesis. They are also associated with a spectrum of diseases ranging from tumor growth to infections to Parkinson’s disease. Cytokines are typically measured either by bioassay or immunoassay.Both techniques are time consuming and can facilitate the analysis of only a single cytokine at a time. For analysis of multiple cytokines, traditional methods can be time consuming, may require large sample volumes and can be very expensive.

Multiplex System for a better and easier Detection—No more ELISAs!

A new multiplex assay for the analysis of multiple proteins in a single well has been pre-tested in our laboratory and is now being offered as a service core. This suspension array system uses multiplexing technology that may one day include up to 100 color-coded bead sets, each of which can be conjugated with a different specific reactant. Each reactant is specific for a different antibody. These reactants are used to create a capture sandwich immunoassay the same as in ELISA assays. The constituents of each well are drawn up into the flow-based Bio-Plex array reader, which identifies each specific reaction based on bead color and can simultaneously quantitate up to all of the protein targets in sera, automatically analyzes samples and increases the amount of useful data per sample.

What we offer:
Cytokine Panels:

Human: We offer a 18-plex human cytokine panel that includes: IL-1 beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12(p70), IL-17,       G-CSF, GM-CSF, IFN- gamma, MCP-1, MIP-1 alpha, MIP-1 beta, RANTES, TNF-alpha, VEGF. and GRO-alpha

Mouse: We offer a 15-plex mouse cytokine panel including IL-1 alpha, IL-1 beta, IL-6, IL-10, IL-12 (p40), IL-12 (p70), G-CSF, GM-CSF, IFN-gamma, KC, MCP-1, MIP-1 alpha, MIP-1 beta RANTES and TNF-alpha

Samples:
Source: cytokines can be evaluated in sera, cell supernatant or cell lysate. 

Volume:only a small sample volume is required, 65ul is required for serum, cell supernatant, or cell lysate.

Cost: 
regular price: $45.00/sample
introductory price for the Yale Community: $15.00/sample

Instructions:

• print and fill out cytoplex order form 

• please email complete form to paula.bole@yale.edu 

• upon confirmation of order, a follow up email will be sent to schedule assay along with further details

Information:

• for further information and orders please please contact Paulomi Aldo at: paula.bole@yale.edu