Core

CytoPlex-Core Facility

Luminex Based Cytokine Detection and Quantification

For analysis of multiple cytokines, traditional methods can be time consuming, may require large sample volumes and can be very expensive. Our core offers a multiplex system for better and easier detection. This multi-plex suspension array system uses a 2 laser, flow-based detection platform.  One laser identifies the bead and the second laser determines the amount of cytokine bound.  Each bead set is coated with a capture antibody specific for a specific cytokine.  A second biotinylated antibody and streptavidin-phycoerythrin (S-PE) is used to detect the captured cytokine creating a capture sandwich immunoassay the same as in ELISA assays.  Cytokines can be evaluated from either human or mouse sera, cell supernatant or cell lysate.

CytoPlex
Cytokine panels available:
  • Human: We offer a 17-plex human cytokine panel that includes: IL-1 beta, IL-6, IL-8, IL-10, IL-12(p70), IL-17, G-CSF, GM-CSF, IFN- gamma, MCP-1, MIP-1 alpha, MIP-1 beta, RANTES, TNF-alpha, VEGF, GRO-alpha, and IP-10
  • Mouse: We now offer a 23-plex mouse cytokine panel that includes: IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6,IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-17, Eotaxin, G-CSF, GM-CSF, IFN-gamma, KC, MCP-1, MIP-1 alpha, MIP-1 beta, RANTES, and TNF-alpha
  • Custom: Please inquire for further details
Cost: Core facility purchases kit of interest, performs assay, reads plate, and sends analysis
  • Sponsored Award Rate/unit - 40/sample
  • Non-Sponsored Rate/unit - 42/sample
  • External Rate/unit - 69/sample
  • For machine use (user must be familiar with or trained on luminex system); customer purchases kit, performs assay, and ONLY uses machine to read plate; Cost - $15.00 per hour

Instructions:

  • Print and fill out cytoplex order form 
  • Please email complete form to paula.bole@yale.edu 
  • Upon confirmation of order, a follow-up email will be sent to schedule assay along with further details

Simple Plex -- Multianalyte Core Facility

Cytokine Detection and Quantification

Validation of cytokine data using traditional methods can be time consuming, may require large sample volumes and can be very expensive. We are now offering hands free, multiplex free, ELISA-free Immunoassay called Simple Plex. 

Simple Plex assays run on Ella let you run multiple samples in approximately 60 minutes and using as little as 25ul of sample. Every step of the assay happens in a ready to go cartridge that includes the wash, incubations, and fully analyzed results for up to 4 analytes in each sample. Because each sample is split across isolated, microfluidic channels this allows for zero cross-reactivity. 

Simple Plex assays give you the same specificity as single-plex ELISA with the advantage of sub-picogram detection limits, a 4-5 log dynamic range and single-digit CVs. Each cartridge holds up to 14-16 samples and there is no need to run a standard curve, they are pre-set from the company.

For further product information and data please visit this website.

Samples: 

  • Source: cytokines can be evaluated from either human or mouse sera, cell supernatant or cell lysate
  • Volume: only a small sample volume, up to 65ul, is required for serum, cell supernatant, or cell lysate
Cost: 
    Validated cartridge price list, pick up to 4 analytes off of the green assay menu, see http://www.proteinsimple.com/simple_plex_assays.html
  • Yale internal only:
    • Sponsored Award Rate/unit - $701
    • Non-Sponsored Rate/unit - $711
  • Non-Yale External customers:
    • External Rate/unit - $1183
    Non-Validated cartridge priced list, pick up to 4 analytes customized off a non-validated list (please inquire for further details)
  • Yale internal only:
    • Sponsored Award Rate/unit - $1001
    • Non-Sponsored Rate/unit - $1010
  • Non-Yale External customers:
    • External Rate/unit - $1682
  • For machine use only:
    • User must be familiar with or trained on Ella 
    • Customer purchases own cartridge, sets up assay, and ONLY uses machine to read plate - $15/run
Instructions:
Information:

In vivo Imaging

The In-Vivo Multispectral FX PRO features automated image capture of multispectral images including fluorescence, X-ray, and white light. These images can then be spatially co-registered for improved localization of biomarkers in vivo. The enhanced sensitivity enables identification and separation of multiple fluorophores and removal of autofluorescent background. Visit http://www.bruker.com/ for more information.

The Multimodal Animal Rotation System (MARS) automatically rotates a mouse the desired angles(s) to capture all relevant molecular and anatomical information with 360-degree coverage. MARS rotation software enables co-registration and visualization of multimodal and multispectral data sets. The MARS allows for quantification of an image at the perfect right angle or exportation of the rotation series as a video file. Visit http://www.bruker.com/ for more information.

All prices are listed as per hour of use for Yale customers:

Services

  1. Start-up training (required for first time users) $50
  2. Basic use, use of machine with no assistance: $41 per hour internal sponsored research; $43 per hour non-sponsored research
  3. Use of machine with technical assistance, $72 per hour in addition to base per hour price
Please note:
  1. Non Yale users cannot be accommodated due to IACUC limitations.
  2. This imager is equipped to handle mouse species only.
  3. Our facility is equipped to handle up to and including BL2 hazards. All hazards must be disclosed on request form prior to use and are subject to approval. It is important to note that once animals have been used in the core imaging facility, you must check YARC and IACUC regulations to determine if your animals may return to your YARC facility.
  4. Use of the imager can begin at 9:00am and must finish by 4pm, weekdays only.
  5. Upon completion of a brief initial training, users will be responsible for imaging independently unless it is indicated that you require technical assistance (additional $75 per hour).
  6. Time must be scheduled the week before use.

Please click here for order form. For additional questions or to send completed order form please email Paulomi Aldo.

Drug Screening

We offer either an in vitro or in vivo model for drug screening. Both models cover multiple targets of potential therapeutics. For more information please download this form or contact Ayesha Alvero.

Image represents our in vivo and in vitrotumor model.