Cell Nucleus; Microscopy, Fluorescence; Microscopy, Confocal; Cellular Structures
Visualizing 3D structure and dynamics at the molecular
scale is a current and critical need in biomedical research. Many sub-cellular
features, for example the morphology of many organelles or the 3D organization
of chromatin, cannot be resolved by standard light microscopy.
Improving the resolution of light microscopy has therefore been an urgent need of biological research for many decades. Today, several methods achieve sub-100 nm resolution by taking advantage of reversible or irreversible photo-physical switching properties of fluorescent markers.
Our research group in the Department of Cell Biology at Yale University School of Medicine is developing new fluorescence microscopy techniques with spatial and/or temporal resolutions exceeding far beyond current technology and also applying them to a diverse set of biological questions.
Extensive Research Description
Our laboratory works on both Stimulated Emission Depletion (STED) microscopy and Fluorescence Photoactivation Localization Microscopy (FPALM/PALM/etc.) techniques. We are actively developing to improve the speed, the 3D resolution and the depth penetration of these imaging techniques to expand the application range of super-resolution microscopy. In collaboration with a diverse set of research groups at Yale University and outside, we apply our new instruments to current biomedical questions.
We are currently working on multiple projects to further improve fluorescence imaging technology and applying these cutting-edge techniques to current biological questions.
- F. Huang, T.M.P. Hartwich, F.E. Rivera-Molina, Y. Lin, W.C. Duim, J.J. Long, P.D. Uchil, J.R. Myers, M.A. Baird, W. Mothes, M.W. Davidson, D. Toomre, J. Bewersdorf (2013) Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms, Nature Methods 10(7): 653-658.
- M.F. Juette, F.E. Rivera-Molina, D.K. Toomre, and J. Bewersdorf (2013) Three-dimensional single particle tracking with adaptive optics reveals cellular millisecond dynamics, Appl. Phys. Lett., 102: 173702.
- T.J. Gould, S.T. Hess, and J. Bewersdorf (2012) Optical Nanoscopy: from Acquisition to Analysis, Annu. Rev. Biomed. Eng. 14: 231–254
- T.J. Gould, D. Burke, J. Bewersdorf, M.J. Booth (2012) Adaptive Optics Enables 3D STED Microscopy in Aberrating Specimens, Opt. Express 20(19): 20998-21009.
- M.F. Juette and J. Bewersdorf (2010) Three-Dimensional Tracking of Single Fluorescent Particles with Submillisecond Temporal Resolution, Nano Lett. 10(11): 4657-4663.
- D. Toomre and J. Bewersdorf (2010) A New Wave of Cellular Imaging, Annu. Rev. Cell Dev. Biol. 26: 285–314.
- M.F. Juette, T.J. Gould, M.D. Lessard, M.J. Mlodzianoski, B.S. Nagpure, B.T. Bennett, S.T. Hess, J.Bewersdorf (2008) Three-dimensional sub-100 nm Resolution Fluorescence Microscopy of Thick Samples, Nature Methods 5(6): 527-529.
- J. Bewersdorf, B.T. Bennett, K.L. Knight (2006) Novel H2AX Chromatin Structures Revealed by 4Pi Microscopy, Proc. Nat. Acad. Sci. 103: 18137-18142.
- H. Gugel, J. Bewersdorf, S. Jakobs, J. Engelhardt, R. Storz and S.W. Hell (2004) Cooperative 4Pi excitation and detection yields 7-fold sharper optical sections in live cell microscopy, Biophys. J. 87: 4146-4152.
- J. Bewersdorf, R. Pick and S.W. Hell (1998) Multifocal Multiphoton Microscopy, Opt. Lett. 23(9): 655-657