Nadia Annitia Ameen MBBS

Associate Professor of Pediatrics (Gastroenterology) and of Cellular and Molecular Physiology

Research Interests

Apical endocytosis and exocytosis; Protein traffic; Secretory diarrhea; Cystic fibrosis


Research Summary


My laboratory has been supported continuously by NIH awards (K08, R03, R01) to study intracellular trafficking routes and mechanisms and that regulate the expression and function of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel in the intestine. CFTR represents the primary exit pathway responsible for anion and fluid secretion on the apical membranes of intestinal cells. Mutations in the CFTR gene result in absence of functional CFTR channels and the genetic disease Cystic Fibrosis while up-regulation of CFTR function is implicated in the pathogenesis of diarrheal disease. We employ transgenic animal and polarized intestinal cell models in conjunction with cell biologic, molecular and physiologic approaches to understand the intracellular trafficking routes traversed by CFTR and how alterations in these pathways lead to intestinal diseases.


Selected Publications

  • Functional vacuolar ATPase (V-ATPase) proton pumps traffic to the enterocyte brush border membrane and require CFTR. Collaco AM, Geibel P, Lee BS, Geibel JP, Ameen NA. Am J Physiol Cell Physiol. 2013 Nov 1;305(9):C981-96. doi: 10.1152/ajpcell.00067.2013. Epub 2013 Aug 28.
  • Characterization of CFTR High Expresser cells in the intestine. Jakab RL, Collaco AM, Ameen NA. Am J Physiol Gastrointest Liver Physiol. 2013 Sep 15;305(6):G453-65. doi: 10.1152/ajpgi.00094.2013. Epub 2013 Jul 18.

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