TM-FISH Table 1

Mouse and human probe combinations for TM-FISH analysis

"Chr" = chromosome number; "c", "t" for mouse clones = the centromeric or telomeric end of the chromosome. "p", "q" for human clones = indicate the p and q arm of the chromosome. F = FITC; R = R6G; N = DNP; B = BIO; D = DIG. Numbers within the table indicate the fluors or haptenes with which the probes for the respective chromosome were labeled. We used numbers, so that chromosome identification is easier. Italic characters of some human clone names indicate the cosmid probes. All other human probes were P1, PACs. When human probes are used according to the 2/3 approach, FITC is replaced by BIO, and R6G by DIG. The probes in these two columns are then hybridized on one area of the slide, whereas the rest of the human clones (the ones always labeled by BIO, DIG, DNP) are hybridized on another area of the slide. Note: to differentiate the chromosomes, information from both hybridizations needs to be combined. #1-#3 = the three probe mixes used for the 3/3 TM-FISH combination. Probes corresponding to chromosomes 4 and 20 (mix #1) and chromosomes 7 and 19 (mix #2), respectively, are differentiated by the size of the fluorescent signal, not by combinatorial labeling.