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Ref: Laboratory Investigation. 2001 Apr;81(4):483-91.Get article in PDF format here
TM-FISH Fig 02 - legend
Fig. 2. a-c. The human 3/3 detection scheme, using all telomere probes combined in three sets (#1 in a, #2 in b and #3 in c). Probes were labeled with BIO, DIG and DNP, thus there were only three initial colors (red, green and blue, identical to the NBD images in Fig 1). The colored dots indicate the expected color of every chromosome pair in each of the three sets and provide the labeling scheme for every chromosome. As in figure 1, red is N, green is D, blue is B, cyan is BD, magenta is NB, yellow is BD and white is NBD. Note that in the 3/3 detection scheme, each probe set detects the chromosomes independently from the other two sets. For chromosomes 20 in set #1 (a) and 19 in set #2 (b), we used ratio labeling for detection. The telomeric probes for these two chromosomes yield signals significantly smaller than the signals for chromosomes 4 (a) and 7 (b), respectively. This obvious signal difference, allows chromosome identification. d-f. Mouse TM-FISH images (1/5 strategy): as all mouse chromosomes are acrocentric, only one probe is close to the telomere, the other one is close to the centromere. Images were prepared as described for the human probes in Fig 1, with FR (in d) and NBD (in e). Arrows point toward the two chromosomes carrying the reciprocal t(4;9) translocation. The reverse DAPI image of the same metaphase is also shown (e). The white letters indicate the positions of the derivative chromosomes 4 (A) and 9 (B) in the metaphase.