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Table 7 (FISH guide)
TOPICS: |1| 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23
FIGURES: | 1 | 2 | 3 | 4 | 5 | 6 |7 | 8 | 9 | 10 | 11 |TABLES: | 1 | 2 | 3 | 4 | 5 | 6 | 7
Table 7.Triple color detection scheme for three haptene labeled probes
Step | BIO (blue detection) | DIG (red detection) | DNP (green detection) |
I. | Avidin AMCA* | Mouse antiDIG |
|
II. | Goat antiavidin BIO* | Sheep antimouse-Cy3 |
|
III. | Avidin AMCA | Donkey antisheepCy3 | Rabbit antiDNP |
IV. | Mouse antiavidin BIO |
| Goat antirabbit-FITC |
V. | Avidin AMCA |
| Donkey antigoat-DTAF |
* first two steps in BIO detection are optional.
(Note: when using AMCA, DAPI counterstaining of chromosomes is not possible, as these two dyes have similar absorption and emission spectra)