CM-FISH Fig. 2 & Table 2 - legends

Table 2. Interphase CM-FISH algorithm. Mixtures C1, C2 and C3, can be used to identify aneuploidies in interphase nuclei (the probe for chromosome 13 in mix C2 identifies both chromosomes 13 and 21). "x" indicates the fluorophore(s) used to identify the various chromosomes. Detection of centromeres with shared alphoid families (group A centromeres) is again achieved by combinatorial plus ratio labeling. "F"=FITC; "3"=Cy3; "B"=BIO (AMCA or Cy7); "5"=Cy5; "D"=DIG/Cy5.5 and "E"=DEAC.a-c.Probe pZ16a (labeled with FITC) hybridizes to the centromeres of 1, 5, 16 and very weak to 19 (a); probe pC1.8 (labeled with Cy3) hybridizes to 1, 5, weaker on 19 and very weak on 16 (b); pG-A16 (labeled with biotin, detected with avidin Cy5) hybridizes to 5, 19 (c). Simultaneous use of these three probes allows identification of all four centromeres (see Fig 1a), based on color combinations plus color ratios. Thus, chromosome 1 centromere will be labeled with FITC, Cy3; chromosome 5 centromere with FITC, CY3, Cy5; chromosome 16 centromere with FITC and very weak Cy3; chromosome 19 centromere with Cy5, Cy3 and weak FITC. d-i. As part of mix C1, the same three probes plus probes for other four centromeres were used in an interphase CM-FISH analysis (Fig 1e-f) to detect a trisomy 8 in a tumor sample.