Figure 6 (slideprep)
Legend:Fig. 5a: illustrates the darker Giemsa staining of metaphases of cells kept in 6:1 fixative (compare with fig. 5). b: reduced trypsin treatment of cells (3 seconds) results in thin chromosomes, uniformly stained by Giemsa, with little banding.c1 and c2: Trypsin treatment of long chromosomes . Long chromosomes (cells were in usual 3:1 fixative) require increased trypsin treatment to yield a better banding pattern. Trypsin time was increased from 30 seconds (c1) to 50 seconds (c2).d1 and d2: Trypsin treatment of short chromosomes. The short chromosomes depicted, were found on the same slides as the longer chromosomes above them, and thus were subjected to the same test of trypsin treatment (30 seconds in d1 and 50 seconds in d2) . Short chromosomes did not respond well to long trypsin treatment, they became more "puffy" and lost the sharp edges. Shorter than 30 seconds trypsin treatment would have been more desirable for these shorter chromosomes.
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Last modified on: Feb12, 2001