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Figure 4 (slideprep)
Slide preparation figures: Fig1 | Fig2 | Fig3 | Fig4 | Fig5 | Fig6 |
Legend:Fig. 4.Pellet resuspended in 1:1 methanol:acetic acid (column a ), 6:1 methanol:acetic acid (column b) and 3:1 ethanol:acetic acid (column c). In row #1, no water vapors, slides dried on the cold end of the metal plate (room temperature); #2, slides exposed to water vapors, then dried at room temperature; #3, no water vapors, slide dried on the hot end of the metal plate; #4, slides exposed to water vapors, dried on the hot end of the metal plate.
The best spreading and cleanest chromosome preparations are achieved in #1 and #4.
