Page designed and maintained by Octavian Henegariu (Email: or ). WARNING: The information provided in these pages is copyrighted and is intended for individual use only. No parts of this work (text, tables or pictures) may be commercialized, published or otherwise reproduced without the written consent of the author. For a complete description of primers, PCR programs and a discussion of the PCR conditions please consult: Andrologia26: 97-106 (1994) and Biotechniques23: 504-511 (1997). Click here to get the Biotechniques paper in PDF format.

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PCR and multiplex PCR: guide and troubleshooting

Designed by OCTAVIAN HENEGARIU, Yale University

Page summary:

• PCR guide: a discussion of the main parameters influencing the outcome of the PCR and multiplex PCR reaction in 16 pages/sections and using over 45 pictures
• PCR troubleshooting: some commonly asked questions and likely solutions
• Standards: provides images of the standard multiplex reactions used during this work. It is useful to consult before reading other pages of the PCR guide
• Applications: includes examples of some applications of PCR and multiplex PCR. Still under construction.