Fig 6 (FISH guide)
LegendFig. 6.Hybridization conditions: cell type, denaturing temperature, hybridization time.Fibroblasts (a-f) and lymphocyte (g-l) slides were subjected to chemical aging in ethanol at 94 C for 30 seconds, then pretreated in pepsin. The first row(a-c) and the third row(g-i) show results after simultaneous denaturing, whereas the second row(d-f) and the forth row(j-l) show results after separate denaturing. In each of the three columns, three different sets of commercial probes were used: two cosmids in the first column and one each in the other two columns. In the first column(a, d, g, j) denaturing was 2 minutes at 76 C and 5 hours hybridization . In the second column(b, e, h, k) denaturing was 2 minutes at 90 C and 5 hours hybridization. In the third column(c, f, i, l), denaturing was 2 minutes at 76 C and 16 hours overnight hybridization. Overall, when chemical aging was used, there were no visible differences between hybridization onto fibroblasts or lymphocytes, between denaturing at 76 or 90 C, between simultaneous or separate denaturing or between hybridization for 5 hours or overnight. Arrows point to the hybridization signals.
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Last modified on: Feb12, 2001