Genomics, Transcriptomics and Proteomics

Kariithi HM, Ince IA, Boeren S, Abd-Alla AM, Parker AG, Aksoy S, Vlak JM, Oers MM. The salivary secretome of the tsetse fly Glossina pallidipes (Diptera: Glossinidae) infected by salivary gland hypertrophy virus. PLoS Negl Trop Dis. 2011 Nov;5(11):e1371. Epub 2011 Nov 22. PubMed PMID: 22132244; PubMed Central PMCID: PMC3222630.

Alves-Silva J, Ribeiro JM, Van Den Abbeele J, Attardo G, Hao Z, Haines LR, Soares MB, Berriman M, Aksoy S, Lehane MJ. An insight into the sialome of Glossina morsitans morsitans. BMC Genomics. 2010 Mar 30;11:213. PubMed PMID: 20353571; PubMed Central PMCID: PMC2853526.

Attardo GM, Ribeiro JM, Wu Y, Berriman M, Aksoy S. Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly (Glossina morsitans morsitans). BMC Genomics. 2010 Mar 9;11:160. PubMed PMID: 20214793; PubMed Central PMCID: PMC2846916.

Attardo GM, Strickler-Dinglasan P, Perkin SA, Caler E, Bonaldo MF, Soares MB, El-Sayeed N, Aksoy S. Analysis of fat body transcriptome from the adult tsetse fly, Glossina morsitans morsitans. Insect Mol Biol. 2006 Aug;15(4):411-24. PubMed PMID: 16907828.

Aksoy S, Berriman M, Hall N, Hattori M, Hide W, Lehane MJ. A case for a Glossina genome project. Trends Parasitol. 2005 Mar;21(3):107-11. Review. PubMed PMID: 15734656.

Rio RV, Lefevre C, Heddi A, Aksoy S. Comparative genomics of insect-symbiotic bacteria: influence of host environment on microbial genome composition. Appl Environ Microbiol. 2003 Nov;69(11):6825-32. PubMed PMID: 14602646; PubMed Central PMCID: PMC262273.

Dávila AM, Majiwa PA, Grisard EC, Aksoy S, Melville SE. Comparative genomics to uncover the secrets of tsetse and livestock-infective trypanosomes. Trends Parasitol. 2003 Oct;19(10):436-9. Review. PubMed PMID: 14519579.

Lehane MJ, Aksoy S, Gibson W, Kerhornou A, Berriman M, Hamilton J, Soares MB, Bonaldo MF, Lehane S, Hall N. Adult midgut expressed sequence tags from the tsetse fly Glossina morsitans morsitans and expression analysis of putative immune response genes. Genome Biol. 2003;4(10):R63. Epub 2003 Sep 11. PubMed PMID: 14519198; PubMed Central PMCID: PMC328452.

Akman L, Yamashita A, Watanabe H, Oshima K, Shiba T, Hattori M, Aksoy S. Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia. Nat Genet. 2002 Nov;32(3):402-7. Epub 2002 Sep 3. PubMed PMID: 12219091.

Akman L, Rio RV, Beard CB, Aksoy S. Genome size determination and coding capacity of Sodalis glossinidius, an enteric symbiont of tsetse flies, as revealed by hybridization to Escherichia coli gene arrays. J Bacteriol. 2001 Aug;183(15):4517-25. PubMed PMID: 11443086; PubMed Central PMCID: PMC95346.

Metabolic and Digestive Biology

Meola S, Sittertz-Bhatkar H, Langley P, Kasumba I, Aksoy S. Abdominal pericardial sinus: a neurohemal site in the tsetse and other cyclorraphan flies. J Med Entomol. 2003 Nov;40(6):755-65. PubMed PMID: 14765650.

Hao Z, Aksoy S. Proventriculus-specific cDNAs characterized from the tsetse, Glossina morsitans morsitans. Insect Biochem Mol Biol. 2002 Dec;32(12):1663-71. PubMed PMID: 12429118.

Yan J, Cheng Q, Narashimhan S, Li CB, Aksoy S. Cloning and functional expression of a fat body-specific chitinase cDNA from the tsetse fly, Glossina morsitans morsitans. Insect Biochem Mol Biol. 2002 Sep;32(9):979-89. PubMed PMID: 12213234.

Yan J, Cheng Q, Li CB, Aksoy S. Molecular characterization of three gut genes from Glossina morsitans morsitans: cathepsin B, zinc-metalloprotease and zinc-carboxypeptidase. Insect Mol Biol. 2002 Feb;11(1):57-65. PubMed PMID: 11841503.

Yan J, Cheng Q, Li CB, Aksoy S. Molecular characterization of two serine proteases expressed in gut tissue of the African trypanosome vector, Glossina morsitans morsitans. Insect Mol Biol. 2001 Feb;10(1):47-56. PubMed PMID: 11240636.

Immunity, Trypanosome and Symbiosis References

Weiss BL, Maltz M, Aksoy S. Obligate symbionts activate immune system development in the tsetse fly. J Immunol. 2012 Apr 1;188(7):3395-403. Epub 2012 Feb 24. PubMed PMID: 22368278; PubMed Central PMCID: PMC3311772.

Rio RV, Symula RE, Wang J, Lohs C, Wu YN, Snyder AK, Bjornson RD, Oshima K, Biehl BS, Perna NT, Hattori M, Aksoy S. Insight into the transmission biology and species-specific functional capabilities of tsetse (Diptera: glossinidae) obligate symbiont wigglesworthia. MBio. 2012 Feb 14;3(1). pii: e00240-11. doi: 10.1128/mBio.00240-11. Print 2012. PubMed PMID: 22334516; PubMed Central PMCID: PMC3280448.

Doudoumis V, Tsiamis G, Wamwiri F, Brelsfoard C, Alam U, Aksoy E, Dalaperas S, Abd-Alla A, Ouma J, Takac P, Aksoy S, Bourtzis K. Detection and characterization of Wolbachia infections in laboratory and natural populations of different species of tsetse flies (genus Glossina). BMC Microbiol. 2012 Jan 18;12 Suppl 1:S3. [Epub ahead of print] PubMed PMID: 22376025; PubMed Central PMCID: PMC3287514.

Symula RE, Marpuri I, Bjornson RD, Okedi L, Beadell J, Alam U, Aksoy S, Caccone A. Influence of host phylogeographic patterns and incomplete lineage sorting on within-species genetic variability in Wigglesworthia species, obligate symbionts of tsetse flies. Appl Environ Microbiol. 2011 Dec;77(23):8400-8. Epub 2011 Sep 23. PubMed PMID: 21948847; PubMed Central PMCID: PMC3233068.

Alam U, Medlock J, Brelsfoard C, Pais R, Lohs C, Balmand S, Carnogursky J, Heddi A, Takac P, Galvani A, Aksoy S. Wolbachia symbiont infections induce strong cytoplasmic incompatibility in the tsetse fly Glossina morsitans. PLoS Pathog. 2011 Dec;7(12):e1002415. Epub 2011 Dec 8. PubMed PMID: 22174680; PubMed Central PMCID: PMC3234226.

Weiss B, Aksoy S. Microbiome influences on insect host vector competence. Trends Parasitol. 2011 Nov;27(11):514-22. Epub 2011 Jun 21. Review. PubMed PMID: 21697014; PubMed Central PMCID: PMC3179784.

Weiss BL, Wang J, Aksoy S. Tsetse immune system maturation requires the presence of obligate symbionts in larvae. PLoS Biol. 2011 May;9(5):e1000619. Epub 2011 May 31. PubMed PMID: 21655301; PubMed Central PMCID: PMC3104962.

Wang J, Wu Y, Yang G, Aksoy S. Interactions between mutualist Wigglesworthia and tsetse peptidoglycan recognition protein (PGRP-LB) influence trypanosome transmission. Proc Natl Acad Sci U S A. 2009 Jul 21;106(29):12133-8. Epub 2009 Jul 8. PubMed PMID: 19587241; PubMed Central PMCID: PMC2715537.

Pais R, Lohs C, Wu Y, Wang J, Aksoy S. The obligate mutualist Wigglesworthia glossinidia influences reproduction, digestion, and immunity processes of its host, the tsetse fly. Appl Environ Microbiol. 2008 Oct;74(19):5965-74. Epub 2008 Aug 8. PubMed PMID: 18689507; PubMed Central PMCID: PMC2565960.

Weiss BL, Wu Y, Schwank JJ, Tolwinski NS, Aksoy S. An insect symbiosis is influenced by bacterium-specific polymorphisms in outer-membrane protein A. Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15088-93. Epub 2008 Sep 24. PubMed PMID: 18815366; PubMed Central PMCID: PMC2567497.

Immunity, Trypanosome and Symbiosis References

Savage AF, Cerqueira GC, Regmi S, Wu Y, El Sayed NM, Aksoy S. Transcript Expression Analysis of Putative Trypanosoma brucei GPI-Anchored Surface Proteins during Development in the Tsetse and Mammalian Hosts. PLoS Negl Trop Dis. 2012 Jun;6(6):e1708. Epub 2012 Jun 19. PubMed PMID: 22724039.

Wang J, Hu C, Wu Y, Stuart A, Amemiya C, Berriman M, Toyoda A, Hattori M, Aksoy S. Characterization of the antimicrobial peptide attacin loci from Glossina morsitans. Insect Mol Biol. 2008 Jun;17(3):293-302. PubMed PMID: 18477243; PubMed Central PMCID: PMC2656931.

Aksoy S, Weiss B, Attardo G. Paratransgenesis applied for control of tsetse transmitted sleeping sickness. Adv Exp Med Biol. 2008;627:35-48. Review. PubMed PMID: 18510012.

Nayduch D, Aksoy S. Refractoriness in tsetse flies (Diptera: Glossinidae) may be a matter of timing. J Med Entomol. 2007 Jul;44(4):660-5. PubMed PMID: 17695022.

Dasgupta R, Free HM, Zietlow SL, Paskewitz SM, Aksoy S, Shi L, Fuchs J, Hu C, Christensen BM. Replication of flock house virus in three genera of medically important insects. J Med Entomol. 2007 Jan;44(1):102-10. PubMed PMID: 17294927.

Weiss BL, Mouchotte R, Rio RV, Wu YN, Wu Z, Heddi A, Aksoy S. Interspecific transfer of bacterial endosymbionts between tsetse fly species: infection establishment and effect on host fitness. Appl Environ Microbiol. 2006 Nov;72(11):7013-21. Epub 2006 Sep 1. PubMed PMID: 16950907; PubMed Central PMCID: PMC1636136.

Hu C, Aksoy S. Innate immune responses regulate trypanosome parasite infection of the tsetse fly Glossina morsitans morsitans. Mol Microbiol. 2006 Jun;60(5):1194-204. PubMed PMID: 16689795.

Rio RV, Wu YN, Filardo G, Aksoy S. Dynamics of multiple symbiont density regulation during host development: tsetse fly and its microbial flora. Proc Biol Sci. 2006 Apr 7;273(1588):805-14. PubMed PMID: 16618673; PubMed Central PMCID: PMC1560226.

Toh H, Weiss BL, Perkin SA, Yamashita A, Oshima K, Hattori M, Aksoy S. Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host. Genome Res. 2006 Feb;16(2):149-56. Epub 2005 Dec 19. PubMed PMID: 16365377; PubMed Central PMCID: PMC1361709.

Aksoy S, Rio RV. Interactions among multiple genomes: tsetse, its symbionts and trypanosomes. Insect Biochem Mol Biol. 2005 Jul;35(7):691-8. Epub 2005 Mar 28. Review. Erratum in: Insect Biochem Mol Biol. 2008 Nov;38(11):1033. PubMed PMID: 15894186.

Hu Y, Aksoy S. An antimicrobial peptide with trypanocidal activity characterized from Glossina morsitans morsitans. Insect Biochem Mol Biol. 2005 Feb;35(2):105-15. PubMed PMID: 15681221.

Lehane MJ, Aksoy S, Levashina E. Immune responses and parasite transmission in blood-feeding insects. Trends Parasitol. 2004 Sep;20(9):433-9. Review. PubMed PMID: 15324734.

Rio RV, Hu Y, Aksoy S. Strategies of the home-team: symbioses exploited for vector-borne disease control. Trends Microbiol. 2004 Jul;12(7):325-36. Review. PubMed PMID: 15223060.

Hao Z, Kasumba I, Aksoy S. Proventriculus (cardia) plays a crucial role in immunity in tsetse fly (Diptera: Glossinidiae). Insect Biochem Mol Biol. 2003 Nov;33(11):1155-64. PubMed PMID: 14563366.

Aksoy S. Control of tsetse flies and trypanosomes using molecular genetics. Vet Parasitol. 2003 Jul 25;115(2):125-45. Review. PubMed PMID: 12878419.

Aksoy S, Gibson WC, Lehane MJ. Interactions between tsetse and trypanosomes with implications for the control of trypanosomiasis. Adv Parasitol. 2003;53:1-83. Review. PubMed PMID: 14587696.

Haines LR, Haddow JD, Aksoy S, Gooding RH, Pearson TW. The major protein in the midgut of teneral Glossina morsitans morsitans is a molecular chaperone from the endosymbiotic bacterium Wigglesworthia glossinidia. Insect Biochem Mol Biol. 2002 Nov;32(11):1429-38. PubMed PMID: 12530210.

Aksoy S, Hao Z, Strickler PM. What can we hope to gain for trypanosomiasis control from molecular studies on tsetse biology? Kinetoplastid Biol Dis. 2002 Jun 6;1(1):4. PubMed PMID: 12234385; PubMed Central PMCID: PMC119325.

Hao Z, Kasumba I, Lehane MJ, Gibson WC, Kwon J, Aksoy S. Tsetse immune responses and trypanosome transmission: implications for the development of tsetse-based strategies to reduce trypanosomiasis. Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12648-53. Epub 2001 Oct 9. PubMed PMID: 11592981; PubMed Central PMCID: PMC60108.

Akman L, Aksoy S. A novel application of gene arrays: Escherichia coli array provides insight into the biology of the obligate endosymbiont of tsetse flies. Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7546-51. Epub 2001 Jun 12. PubMed PMID: 11404467; PubMed Central PMCID: PMC34705.

Aksoy S, Maudlin I, Dale C, Robinson AS, O'Neill SL. Prospects for control of African trypanosomiasis by tsetse vector manipulation. Trends Parasitol. 2001 Jan;17(1):29-35. PubMed PMID: 11137738.

Cheng Q, Ruel TD, Zhou W, Moloo SK, Majiwa P, O'Neill SL, Aksoy S. Tissue distribution and prevalence of Wolbachia infections in tsetse flies, Glossina spp. Med Vet Entomol. 2000 Mar;14(1):44-50. PubMed PMID: 10759311.

Aksoy S. Tsetse--A haven for microorganisms. Parasitol Today. 2000 Mar;16(3):114-8. Review. PubMed PMID: 10689331.

Cheng Q, Aksoy S. Tissue tropism, transmission and expression of foreign genes in vivo in midgut symbionts of tsetse flies. Insect Mol Biol. 1999 Feb;8(1):125-32. PubMed PMID: 9927181.

Chen X, Li S, Aksoy S. Concordant evolution of a symbiont with its host insect species: molecular phylogeny of genus Glossina and its bacteriome-associated endosymbiont, Wigglesworthia glossinidia. J Mol Evol. 1999 Jan;48(1):49-58. PubMed PMID: 9873076.

Aksoy S, Chen X, Hypsa V. Phylogeny and potential transmission routes of midgut-associated endosymbionts of tsetse (Diptera:Glossinidae). Insect Mol Biol. 1997 May;6(2):183-90. PubMed PMID: 9099582.

Aksoy S. Wigglesworthia gen. nov. and Wigglesworthia glossinidia sp. nov., taxa consisting of the mycetocyte-associated, primary endosymbionts of tsetse flies. Int J Syst Bacteriol. 1995 Oct;45(4):848-51. PubMed PMID: 7547309.

Aksoy S, Pourhosseini AA, Chow A. Mycetome endosymbionts of tsetse flies constitute a distinct lineage related to Enterobacteriaceae. Insect Mol Biol. 1995 Feb;4(1):15-22. PubMed PMID: 7742973.

Aksoy S. Molecular analysis of the endosymbionts of tsetse flies: 16S rDNA locus and over-expression of a chaperonin. Insect Mol Biol. 1995 Feb;4(1):23-9. PubMed PMID: 7538012.

O'Neill SL, Gooding RH, Aksoy S. Phylogenetically distant symbiotic microorganisms reside in Glossina midgut and ovary tissues. Med Vet Entomol. 1993 Oct;7(4):377-83. PubMed PMID: 8268495.

Beard CB, O'Neill SL, Mason P, Mandelco L, Woese CR, Tesh RB, Richards FF, Aksoy S. Genetic transformation and phylogeny of bacterial symbionts from tsetse. Insect Mol Biol. 1993;1(3):123-31. PubMed PMID: 8269090.

Reproductive Biology

Attardo GM, Benoit JB, Michalkova V, Yang G, Roller L, Bohova J, Takáč P,Aksoy S. Analysis of lipolysis underlying lactation in the tsetse fly, Glossina morsitans. Insect Biochem Mol Biol. 2012 May;42(5):360-70. PubMed PMID: 22509523.

Benoit JB, Attardo GM, Michalkova V, Takac P, Bohova J, Aksoy S. Sphingomyelinase Activity in Mother's Milk Is Essential for Juvenile Development: A Case from Lactating Tsetse Flies. Biol Reprod. 2012 Apr 18. [Epub ahead of print] PubMed PMID: 22517621.

Benoit JB, Yang G, Krause TB, Patrick KR, Aksoy S, Attardo GM. Lipophorin acts as a shuttle of lipids to the milk gland during tsetse fly pregnancy. J Insect Physiol. 2011 Nov;57(11):1553-61. Epub 2011 Aug 22. PubMed PMID: 21875592; PubMed Central PMCID: PMC3209505.

Yang G, Attardo GM, Lohs C, Aksoy S. Molecular characterization of two novel milk proteins in the tsetse fly (Glossina morsitans morsitans). Insect Mol Biol. 2010 Apr;19(2):253-62. Epub 2010 Feb 1. PubMed PMID: 20136662; PubMed Central PMCID: PMC2862765.

Attardo GM, Lohs C, Heddi A, Alam UH, Yildirim S, Aksoy S. Analysis of milk gland structure and function in Glossina morsitans: milk protein production, symbiont populations and fecundity. J Insect Physiol. 2008 Aug;54(8):1236-42. Epub 2008 Jul 4. PubMed PMID: 18647605; PubMed Central PMCID: PMC2613686.

Hu C, Rio RV, Medlock J, Haines LR, Nayduch D, Savage AF, Guz N, Attardo GM, Pearson TW, Galvani AP, Aksoy S. Infections with immunogenic trypanosomes reduce tsetse reproductive fitness: potential impact of different parasite strains on vector population structure. PLoS Negl Trop Dis. 2008 Mar 12;2(3):e192. PubMed PMID: 18335067; PubMed Central PMCID: PMC2265429.

Guz N, Attardo GM, Wu Y, Aksoy S. Molecular aspects of transferrin expression in the tsetse fly (Glossina morsitans morsitans). J Insect Physiol. 2007 Jul;53(7):715-23. Epub 2007 Apr 1. PubMed PMID: 17498733; PubMed Central PMCID: PMC2065764.

Strickler-Dinglasan PM, Guz N, Attardo G, Aksoy S. Molecular characterization of iron binding proteins from Glossina morsitans morsitans (Diptera: Glossinidae). Insect Biochem Mol Biol. 2006 Dec;36(12):921-33. Epub 2006 Sep 26. PubMed PMID: 17098167; PubMed Central PMCID: PMC1698469.

Attardo GM, Guz N, Strickler-Dinglasan P, Aksoy S. Molecular aspects of viviparous reproductive biology of the tsetse fly (Glossina morsitans morsitans): regulation of yolk and milk gland protein synthesis. J Insect Physiol. 2006 Nov-Dec;52(11-12):1128-36. Epub 2006 Sep 5. PubMed PMID: 17046784; PubMed Central PMCID: PMC1779500.

Salivary Gland Biology

Haddow JD, Poulis B, Haines LR, Gooding RH, Aksoy S, Pearson TW. Identification of major soluble salivary gland proteins in teneral Glossina morsitans morsitans. Insect Biochem Mol Biol. 2002 Sep;32(9):1045-53. PubMed PMID: 12213241.

Li S, Kwon J, Aksoy S. Characterization of genes expressed in the salivary glands of the tsetse fly, Glossina morsitans morsitans. Insect Mol Biol. 2001 Feb;10(1):69-76. PubMed PMID: 11240638.

Li S, Aksoy S. A family of genes with growth factor and adenosine deaminase similarity are preferentially expressed in the salivary glands of Glossina m. morsitans. Gene. 2000 Jul 11;252(1-2):83-93. PubMed PMID: 10903440.

Cappello M, Li S, Chen X, Li CB, Harrison L, Narashimhan S, Beard CB, Aksoy S. Tsetse thrombin inhibitor: bloodmeal-induced expression of an anticoagulant in salivary glands and gut tissue of Glossina morsitans morsitans. Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14290-5. PubMed PMID: 9826693; PubMed Central PMCID: PMC24366.

Cappello M, Bergum PW, Vlasuk GP, Furmidge BA, Pritchard DI, Aksoy S. Isolation and characterization of the tsetse thrombin inhibitor: a potent antithrombotic peptide from the saliva of Glossina morsitans morsitans. Am J Trop Med Hyg. 1996 May;54(5):475-80. Erratum in: Am J Trop Med Hyg 1996 Jul;55(1):118. PubMed PMID: 8644901.

Population Genetics and Ecology

Ouma JO, Beadell JS, Hyseni C, Okedi LM, Krafsur ES, Aksoy S, Caccone A. Genetic diversity and population structure of Glossina pallidipes in Uganda and western Kenya. Parasit Vectors. 2011 Jun 28;4:122. PubMed PMID: 21711519; PubMed Central PMCID: PMC3146932.

Bonomi A, Bassetti F, Gabrieli P, Beadell J, Falchetto M, Scolari F, Gomulski LM, Regazzini E, Ouma JO, Caccone A, Okedi LM, Attardo GM, Guglielmino CR, Aksoy S, Malacrida AR. Polyandry is a common event in wild populations of the Tsetse fly Glossina fuscipes fuscipes and may impact population reduction measures. PLoS Negl Trop Dis. 2011 Jun;5(6):e1190. Epub 2011 Jun 7. PubMed PMID: 21666797; PubMed Central PMCID: PMC3110164.

Davis S, Aksoy S, Galvani A. A global sensitivity analysis for African sleeping sickness. Parasitology. 2011 Apr;138(4):516-26. Epub 2010 Nov 16. PubMed PMID: 21078220; PubMed Central PMCID: PMC3282146.

Echodu R, Beadell JS, Okedi LM, Hyseni C, Aksoy S, Caccone A. Temporal stability of Glossina fuscipes fuscipes populations in Uganda. Parasit Vectors. 2011 Feb 14;4:19. PubMed PMID: 21320301; PubMed Central PMCID: PMC3045980.

Beadell JS, Hyseni C, Abila PP, Azabo R, Enyaru JC, Ouma JO, Mohammed YO, Okedi LM, Aksoy S, Caccone A. Phylogeography and population structure of Glossina fuscipes fuscipes in Uganda: implications for control of tsetse. PLoS Negl Trop Dis. 2010 Mar 16;4(3):e636. PubMed PMID: 20300518; PubMed Central PMCID: PMC2838784.

Abila PP, Slotman MA, Parmakelis A, Dion KB, Robinson AS, Muwanika VB, Enyaru JC, Okedi LM, Aksoy S, Caccone A. High levels of genetic differentiation between Ugandan Glossina fuscipes fuscipes populations separated by Lake Kyoga. PLoS Negl Trop Dis. 2008 May 28;2(5):e242. Erratum in: PLoS Negl Trop Dis. 2008;2(6). doi: 10.1371/annotation/bc182916-f740-4366-b50e-80410649f197.. Lokedi, Loyce M [corrected to Okedi, Loyce M A]. PubMed PMID: 18509474; PubMed Central PMCID: PMC2386243.

Luna C, Bonizzoni M, Cheng Q, Robinson AS, Aksoy S, Zheng L. Microsatellite polymorphism in tsetse flies (Diptera: Glossinidae). J Med Entomol. 2001 May;38(3):376-81. PubMed PMID: 11372961.