Molecular Tests Performed

Analyte

Method

Source/ status

Sample types

Time to result*

HIV-1 RNA

Quantitative Real-time COBAS TaqMan RT-PCR

Roche
IVD (FDA approved)

Plasma

2-3 days

HIV-1 resistance genotyping

TruGene Sequencing

Bayer
IVD (FDA approved)

Plasma

1-2 weeks

HIV-1 DNA

Qualitative DNA PCR, detected by microplate hybridization

Roche RUO

PBMC

3-4 days

HCV RNA

Quantitative Real-time COBAS TaqMan RT-PCR

Roche
IVD (FDA approved)

Serum

4-7 days

HCV genotype

Line Probe Assay

Siemens

Serum

1-7 days

HBV DNA

Quantitative Real-time COBAS TaqMan RT-PCR

Roche ASR

Serum

4-7 days

HSV DNA

Real-time TaqMan PCR; typing by multiplex PCR qualitative and quantitative

Home-brew

CSF, ocular fluid,  plasma

5 hrs-3 days

VZV DNA

Real-time TaqMan PCR

Home-brew

CSF, ocular fluid, leukocytes

5 hrs-3 days

CMV DNA

Real-time TaqMan PCR, qualitative and quantitative

Home-brew

CSF, ocular fluid, plasma, amniotic fluid

5 hrs-3 days

EBV DNA

Real-time TaqMan PCR

Home-brew

CSF, ocular fluid, plasma

5 hrs-3 days

HHV-6 DNA

Real-time TaqMan PCR qualitative and quantitative

Home-brew

CSF, plasma

5 hrs-3 days

Parainfluenza types 1,2,3, RNA
Real-time TaqMan RT-PCR (one step)Home-brew
Respiratory samples5 hrs-3 days

Parvovirus B19
DNA

Real-time TaqMan PCR

Home-brew

Serum, bone marrow, amniotic fluid, CSF

5 hrs-3 days

Human metapneumovirus

Real-time TaqMan RT-PCR (one step)

Home-brew

Respiratory samples

5 hrs-3 days

Enterovirus RNA

Real-time TaqMan RT-PCR (two step)

Home-brew

CSF, stool, throat, NP, serum

5 hrs-3 days

JC virus DNA

Real-time TaqMan PCR

Home-brew

CSF

5 hrs-3 days

BK virus DNA

Real-time TaqMan PCR, qualitative and quantitative

Home-brew

Plasma, urine

5 hrs-3 days

Influenza A and B
RNA

Real-time TaqMan RT-PCR (one step), multiplex

Home-brew

Respiratory samples

5 hrs-3 days

Influenza A Subtype

Real-time TaqMan RT-PCR (one step)H1, H3, Swine H1

Home-brew

Respiratory samples

5-24 hrs

Adenovirus

Real-time TaqMan PCR, qualitative and quantitative

Home-brew

Many

5 hrs-3 days

Norovirus RNA

Real-time TaqMan RT-PCR (two step), multiplex for genogroups I and II

Home-brew

stool

5 hrs-3 days

RSV RNA

Real-time TaqMan RT-PCR (one step)

Home-brew

Respiratory samples

5 hrs-3 days

Rhinovirus RNA

Real-time TaqMan RT-PCR (one step)

Home-brew

Respiratory samples

5 hrs-3 days

  • Most molecular tests are done Monday-Friday, and are not done on weekends or holidays, when staffing is minimal. The exceptions are HSV PCR and EV RT-PCR on CSF, which are done on Saturdays. Other CSF analytes or high priority samples will also be done when possible.
  • IVD = in vitro diagnostic; RUO= research use only; ASR = analyte specific reagent