9. Identification of Mycobacteria

  • Growth rate
  • Temperature range of growth
  • Colony morphology
  • Colony pigment
  • Biochemical tests
  • Ribosomal RNA probes
  • High Performance Liquid Chromatography (HPLC)

Organism Characteristics

  • Growth in three days on Blood agar at 37°C
  • Color:
    • Tan, buff
    • No change with light exposure
  • Colony morphology: rough
    Initial impression: Rapid grower (Runyun Group IV)

Identification of Organism

  • Sent to West Haven Veteran's Administration Medical Center for HPLC - central laboratory for system
  • HPLC technique based on species-specific characteristics of mycolic acids in the cell wall